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The technology of separation and purification of the interesting protein was studied, centrifuge the sample and get the clear supernatant, remove protein by 50％ ethanol, concentrate by drying with freezing way, remove salt and pigment by dialysing, separation with G-50 and CM-cellulose52 column, concentrate by PEG6000, The purified sample was a single band after separation by SDS-PAGE.

研究了目的阿片肽的分离纯化工艺。发酵混合物经过离心取上清，乙醇沉淀去蛋白，冷冻干燥浓缩，透析除盐脱色，聚乙二醇6000反渗透浓缩，G-50柱分离，CM-cellulose52阳离子交换层析，聚乙二醇6000浓缩等步骤后得到了较纯的目的产物，目的产物在SDS-PAGE上呈现单一条带。

The analytical parameters including pH value, sample volume, flow rate of sample solution and elution conditions (concentration, volume and flow rate of eluant) were investigated. The effect of foreign ions and the adsorption capacity of the modified peanut shell were examined.

考察了样品pH值、进样体积、流速、洗脱剂浓度、体积、流速等参数的影响，同时研究了共存离子的影响，评价了萃取材料的吸附容量及再生性能。

After 10h ball milling, the alloy of carbonyl iron and carbonyl nickel began to form, and the sample kept its original bcc and fcc structure; after 100h ball milling, the sample became a solid solution with bcc structure; after 200h ball milling, the diffraction peak of Ni almost disappeared and the isotropic grains with D achieved, which was mainly attributed to the dislocations and disclinations of the nanocrystalline bcc FeNi suffering from severe plastic deformation by ball milling.

球磨10h时，羰基铁和羰基镍开始形成合金，但大部分Fe、Ni原子仍分别以bcc结构和fcc结构存在。球磨100h时，样品以bcc结构的FeNi合金为主，球磨200h后，样品中fcc结构Ni特征峰几乎消失，FeNi纳米晶达到最小粒径的平衡值，~8nm。高能球磨过程中，晶界区域的层错缺陷是合金化和纳米晶形成的重要因素。

We find on enquiry to the foreign trade company the air freight and broker charge of the sample machine will be USD330. The sample machine will be provided by us free of charge. If you trust us, you can remit us the payment in any way. We will dispatch it upon receipt of the remittance.

经咨询外贸公司，空运样机需要运费及报关费用330USD，样机由我厂免费提供，如果您信任我厂，可以通过任何形式汇款至我厂后，马上发货。

The sample was diluted and passed through the DIONEX On Guard Ⅱ RP sample pretreatment cartridge at a flow rate of 4mL/min to wipe off fat and other hydrophobic compound, then was filtered by 0.2μm filters and was determined directly.

将样品稀释后以4mL/min的流速通过DI- ONEX OnGuardⅡRP固相萃取柱去除脂肪等疏水性化合物后，经0.2μm滤膜过滤，直接进样测定。

The conclusion is drawn from the simulations that the secondary electrons will go out from the sample surface more easily with the decrease of the half cone angle of tip and the increase of secondary electron energy, tip-sample separation and the distance of the emission site away from the point directly under the tip.

模拟的结果发现针尖半锥角越小、次级电子能量越高、针尖样品间距越大、次级电子出射位置离针尖正下方越远则越有利于次级电子的逸出，此外通过栅网与一个平行于样品表面的地电极的共同作用，会对次级电子产生聚焦作用。

Factors influencing the tensile strength of polymerizer head sample were introduced,which included the sampling position,sampling direction,sampling quantity and the cross section shape of sample.

介绍了影响聚合釜封头试样拉伸性能的因素：取样部位、取样方向和取样数量及试样的横截面形状。

The result also showed that the heat-treatment temperature and time had great influence on the electrochemical property of the sample, the performance of the sample was better when it was heat-treated for 20 hours at 120℃.

另外，研究结果表明，样品的热处理温度与时间对样品的电化学性能有较大的影响，在120℃热处理20h的样品性能稳定。

2The top sample of acetaldehyde column is 98% acetaldehyde production. Thebottom sample is the mixture of ketone and alcohol.

乙醛塔：塔顶得到纯度为 98％的乙醛产品，塔釜为醇﹑酮混合物。

Chemical and microbiological compositions of 12 samples of traditionally home-made koumiss in Xingjiang Uighur Autonomous Region were determined. The average contents of fat, protein, lactose, ash, calcium and phosphorus were 2.07g/100mL, 2.22g/100mL, 3.03g/100mL, 0.28g/100mL, 82.27mg/100mL and 58.20mg/100mL respectively. On average, pH was 3.90, titratableacidity was 134.25°T, lactic acid content was 10.99g/L, acetic acid content was 1.50g/L, citric acid content was 0.09g/L, ethanol content was 14.94g/L. The counts of lactic bacteria in 10 samples were 1.07～9.70×107cfu/mL, 1 sample was 1.38×108cfu/mL, another sample was 2.06×106cfu/mL; the counts of yeast in 1 samples was 3.20×104cfu/mL, 5 samples were 1.80～6.60×105cfu/mL, and 6 samples were 1.06～2.72×106cfu/mL. 33 strains isolated from 12 samples of traditionally home-made koumiss collected from Xingjiang Uighur Autonomous region belonged to Lactobacillus.

本项研究，对采集于新疆伊犁哈萨克族自治州、博尔塔拉蒙古族自治州和巴音郭愣蒙古族自治州 3 个地区的 12 份酸马奶样品，进行了化学和微生物组成分析，并对从中分离到的 33 株杆菌分离株，进行了属种鉴定，同时还对耐酸性菌株进行了初步筛选，结果表明： 12 份酸马奶样品的脂肪平均含量为 2.07g/100mL ，蛋白质平均含量为2.22g/100mL，乳糖平均含量为 3.03g/100mL，灰分平均含量为 0.28g/100mL，钙平均含量为 82.27mg/100mL，磷平均含量为 58.20mg/100mL；pH 值平均为 3.90，酸度平均为 134.25oT，乳酸平均含量为 10.99g/L，乙酸平均含量为 1.50g/L，4 份未检出，8 份柠檬酸平均含量为 0.09g/L，乙醇平均含量为 14.94g/l；1 份乳酸菌数为1.38×108cfu/mL，1 份为 2.06×106cfu/mL，其余 8 份在 1.07～9.70×107cfu/mL 间；1份酵母菌数为 3.20×104cfu/mL，5 份在 1.80～6.60×105cfu/mL 间，6 份在 1.06～2.72×106 cfu/mL 间。

Pachomius manual labour was organized as an essential part of the monastic life; and since it is a principle of the monks as distinguished from the mendicants, that the body shall be self-supporting, external work of one sort or another has been an inevitable part of the life ever since.

根据圣pachomius体力劳动组织作为一个重要组成部分寺院生活；以来，这是一个原则的和尚，作为有别于乞丐，该机构应自支撑，对外工作的这种或那种已一个必然的一部分，生活至今。

In order to study the effects of parametrical rolling on the ship movement and tilts, in allusion to ship's navigating in regular following waves, the equation of ship rolling motion is transformed into normal Mathieu equation by proper variable replacement when strong parametrical excitation in restoring moment varied according to sine or cosine wave.

为研究参数横摇对船舶运动和倾覆的影响，通过对船在规则波上随浪航行时，恢复力矩中出现的1个以正余弦规律变化的强参数激励项进行适当的变量置换，将船舶的横摇运动方程转换成标准形式的Mathieu方程。