查询词典 one-step process

Design another set of specific primers, of which upstream and downstream primers were located in the two sides of the cleavage site respectively, to develop the one-step RT-PCR method for detecting H5 and H9 subtypes of AIV. Identify the sensitivity of the developed one-step RT-PCR method by detecting 2 AIV strains of H5 subtype and 3 AIV strains of H9 subtype, and the specificity by detecting the RNAs of NDV, IBV and IBDV as well as analogue detection using computer software.

根据H5、H9亚型HA基因序列，分别设计一套特异性引物，其上、下游引物分别位於裂解位点两侧，应用RT-PCR一步法检测AIV H5和H9亚型，并验证所建立方法的敏感性；通过检测NDV、IBV和IBDV等RNA病毒及电脑软件模拟检测，验证其特异性。

Third chapter illustrate the design scheme for justification development system, amalgamation steer the argumentum;And code design in the detailed design sum the database design of whole system;Recommended the detailed design information of sum of the shuttering cake structure of the aetioporphyrin system;Analyzed the library to manage the system's appliance demand, according to the database design theory proptosis of one-step and one-step earth joint system demand manual, local ER general view, overall situation ER general view, system relation modal, leaven the modal;Arrangement in parallel proptosis the valve key source of the said system code in the procedure the amalgamation commit the interpretation, justification.

第三章说明开发系统的设计方案，并进行论证；以及详细设计中的代码设计和整个系统的数据库设计；介绍了本系统的模块结构和详细的设计信息；分析了图书馆管理系统的应用需求，按照数据库设计理论一步一步地给出了系统需求说明书、局部ER图、全局ER图、系统关系模式，子模式；并列出了该系统的关键源程序中的代码并做解释、说明。

In the second chapter, elementary theories and ideas of one step forming inverse approach were described and warped modification method, implemented by Cook, was introduced into one step forming simulation and based on idea of surface spreading; new quadrilateral element modification arithmetic was put forward. By spreading warped quadrilateral onto plane in local coordinate system, a plane quadrilateral element whose shape and area were equal to original warped quadrilateral element was gotten and internal force vectors of four nodes and stiffness matrix of elements were improved to increase precision of simulations on this plane element.

第二章从一步成形逆有限元法的基本理论和思想出发，将Cook基于弹性理论的四边形单元的翘曲修正算法引入基于塑性的一步成形，并且基于曲面展开的思想，本文提出一种新的四节点单元翘曲修正方案，通过把翘曲的单元展开的方法在局部参考系中得到一个与实际翘曲单元的形状与面积非常接近的平面单元，在该平面单元中进行单元刚度及内力向量的计算，进一步改善模拟结果的精度。

"One step at a time," Arum said,"one step at a time."

"一步一个脚印，"阿鲁姆说。

The results showed that there was no significant difference in three structure approach of liquid layers among which B was relatively higher;and it was considered that one-step dilution was an effective preservation method in that survival capacity with frozen and melted embryos freshly split,and survival capacity of frozen and melted IVF embryos split by one-step dilution by application minimum capacity refrigerant method with specific canaliculus were obviously higher than B.

结果，3种不同液体层构成间胚胎的存活能力无显著差异，B法较高；应用特定细管最小容量冷却法在冷冻溶解的新鲜分割胚、体外受精分割胚及冷冻溶解的体外受精分割胚一步法稀释后的存活能力均显著高于B法。结果表明，特定细管最小容量冷却法是更有效的保存方法。

In addition,the authors propose that for a multi peaked one dimensional search problem its optimum solution can be obtained by ordinary optimization methods only when the convexity or concavity of the objective function matches the step length determining the one dimensional search region and the method of finding the optimum step length factor.

另外 ，还提出了对于多峰一维搜索问题，只有当所求函数的凹凸性与确定一维搜索区问题的步长以及求解最优步长因子的方法匹配时，才能用一般方法得到搜索区间内的最优解

Declaimer in to conceal illiterate fact one step by step the Han Na of be reduced to poverty, the extreme that is this one concept only appears.

朗读者》中为掩盖不识字真相而一步步沦落的汉娜，只是这一概念的极端呈现。

This is we should let global information can be visited and use the one half step that strides, but also be important one step.

这是我们要让全球的信息都能够访问和利用迈出的一小步，但是也是重要的一步。

After the fixed position that has become oneself, release handpick content, be the most important one step next, the one step that the development of all websites does not bypass---Promotion, also be among them a the most difficult link.

在做好自己的定位后，发布精选的内容，然后就是最重要的一步了，所有网站的发展都绕不开的一步---推广，也是其中最难的一个环节。

Soybean ; Bean pod mottle virus ; one step real-time fluorescent RT-PCR ; one step RT-PCR ; detection

大豆；菜豆荚斑驳病毒；一步法实时荧光RT-PCR ；一步法RT-PCR ；检测

I hope that this success is just the first in a long series.

我希望这只是成功的开始。

Three dimensional stresses on the interface of tire and soil, tire deflection and soil sinkage are measured.

测试轮胎——沙土界面上的三向应力、轮胎变形和沙土沉陷；建立考虑剪切应力对沙土承载特性产生影响的车轮牵引性能预测模型；分析车轮作用下土壤的纵向稳定性。