查询词典 one-step process

Sensitivity comparison between the novel one-step RT-PCR and DAS-ELISA for PVX revealed that the former method was 20 times more sensitive than the later one. The novel one-step RT-PCR system was also successfully tested with field samples of turnip mosaic virus in 8 different crucifer vegetable crops.

为验证该设想，本研究建立了一步RT-PCR技术扩增体系，并利用该体系扩增和分析了国内几种蔬菜作物部分CMV分离物的2b基因序列同源性，选择特定分离物的全长2b基因，先利用PVX侵染性载体初步分析其正义和反义序列间的互作，再构建植物表达载体转化烟草和番茄，并研究转基因烟草抗CMV的表现，获得了抗CMV的转基因烟草植株。

The third point, most difficult of all to understand, is that students of history seem intentionally to refuse to see that this march cannot be ascribed to any one man, that no one foresaw it at any time, that, like the retreat to Fili, the manuvre was, in reality, never conceived of by any one in its entirety, but arose step by step, incident by incident, moment by moment from a countless multitude of the most diverse circumstances, and is only conceived of in its entirety, when it is an accomplished fact, and has become the past.

第三，令人最难以理解的是，研究历史的人故意不愿看见，这次侧翼进举不能归功于任何一个人，在任何时候都没有任何一个人对它有所预见，从菲利的撤退也和它完全一样，在任何时候都没有任何一个人看清楚它的全貌，它是由无数的各种各样的条件一步一步地、一个事件接着一个事件、随着时间的推移逐渐显露出来的，只有当它已经完成和已经成为过去的时候，它的全貌才呈现出来。

For drawing the parameter curve by point to point it orde to the parameter t's step length of increased make the progress range of the curve can not large than the length of one pels, otherwise the curve may step over one middle pels and skip one point that it should be on the curve.

要逐点地生成参数曲线，就要求参数t每次增加的步长要使曲线前进的幅度不得超过一个象素长度，否则有可能跨过一个中间象素而产生断点。

But as the programs went on ,we learnt to walk the parade step and the route step which you need physical coordination ,or you'll look strange when you ara walking ,not very bad except not in tune with others walking around you And we were going to take part in review before the general ,however only 50%of us could have the chance to attend it as a square matrix ,the others will only be the spectators ,to tell the truth I was almost one of the spectators ,but as a guy who lack of compatibility 'cause my muscles were too nervous which made the drillmasters hesitate ,my drillmaster told the other one that that guy was the most hardworking one .

但是随着训练的进行，我们又学习了走便步和正步，这都需要身体的协调性，否则你会看起来有一点奇怪，不能算错，就是和周围的人不协调，而我们要去参加阅兵式接受首长的检验，然而只有一半的人可以作为一个方阵参加，其他的人就只能作为观众了，说实在的我几乎已经是观众中的一个了，但是作为一个所谓的身体不协调者，主要是因为我的肌肉太过于紧张导致的，我让教官们感觉到很犹豫不决，但是我的教官对另外一个说，这个孩子训练是最刻苦的，对方说，是的，我知道，你说呢？

Custom is a thing that no one can throw it out of the window but lead it to the downstair one step by one step

人的行为准则是，维护自己的尊严，不妨碍他人的自由。

So one man is described as generous, another as a miser; one is called open-handed, another tight-fisted; one man is cruel, another gentle; one untrustworthy, another reliable; one effeminate and cowardly, another bold and violent; one sympathetic, another self-important; one promiscuous, another monogamous; one straightforward, another duplicitous; one tough, another easy-going; one serious, another cheerful; one religious, another atheistical; and so on.

因而，说此人慷慨大方，彼人是吝啬鬼；此人出手大方，彼人一毛不拔；此人冷酷无情，彼人和蔼可亲；此人不可信赖，彼人忠诚可靠；此人柔弱胆怯，彼人勇猛凶悍；此人同情他人，彼人惟我独尊；此人荒淫无度，彼人洁身自爱；此人刚正不阿，彼人心地奸诈；此人固执己见，彼人一团和气；此人不苟言笑，彼人开朗乐观；此人虔信宗教，彼人心无上帝；如此等等。

Connection Panel: One DVI port, one VGA input, one VGA monitor pass-through, one composite video, one s-video, one USB port for mouse control, one RJ-45 wired networking port, one 1/8" audio input, one L/R RCA audio input, one 1/8" audio output, one RS-232 port, Kensington lock point.

连接面板：一个DVI端口，一个VGA输入，一个VGA监视器传递，一个复合视频，一个S端子，一个USB端口的鼠标控制，一个使用RJ - 45有线网络端口，一个1 / 8 "音频输入，一个左/右的RCA音频输入，一个1 / 8 "的音频输出，一个RS - 232端口，肯辛顿锁点。

Faith Evans My heart belongs to you So what could I do To make you feel I'm down with You see me hangin around But you don't know how you make me feel for you, and Each and every day, I try to make some sense of this What you mean to me, I know it could be serious Each and every nite, I dream about just holding you Loving you like this, what is a girl supposed to do I love you I want you You're the one that I live for And I can't take it any more I love you I need you What can I do to make you see You're the only one for me First time I saw your face My heart just erased All the guys I knew before You walked into my life I was the type to never want for nothing Each and every day, I try to make some sense of this What you mean to me, I know it could be serious Each and every nite, I dream about just holding you Loving you like this, what is a girl supposed to do I love you I want you You're the one that I live for And I can't take it any more I love you I need you What can I do to make you see You're the only one for me I love you, I want you, I need you in my life Can't you see what you mean to me Baby come hold me tight I miss you, wanna kiss you Everytime I see your face Baby I'll be waiting for you Each and everyday I love you I want you You're the one that I live for And I can't take it any more I love you I need you What can I do to make you see You're the only one for me You're the only one for me And I know it's meant to be What can I do to make you see My love will carry on So listen to my heart and know You'll find out where my love will go The future lies between us boy Oh babe I love you so I love you I want you You're the one that I live for And I can't take it any more I love you I need you What can I do to make you see You're the only one for me I love you I want you You're the one that I live for And I can't take it any more I love you I need you What can I do to make you see You're the only one for me

我的心属于你如此我可以做什么使你感觉我是下来由于你在附近见到我 hangin 但是你不知道你如何使我同情你，和每个和每个日子，我试着制造一些感觉这你对我意谓的，我知道它可能是严重的每个和每 nite，我梦到仅仅捉住你亲爱的你喜欢这，女孩应该做什么我爱你我想要你你是那一个我活的为而且我再不能轮流它我爱你我需要你我能做使你见到什么你是我的唯一一个第一时间我见到你的脸我的心刚刚抹掉所有的家伙我以前知道你走进入我的生活我是类型从不免费想要每个和每个日子，我试着制造一些感觉这你对我意谓的，我知道它可能是严重的每个和每 nite，我梦到仅仅捉住你亲爱的你喜欢这，女孩应该做什么我爱你我想要你你是那一个我活的为而且我再不能轮流它我爱你我需要你我能做使你见到什么你是我的唯一一个我爱你，我想要你，我在我的生活方面需要你你不能理解你对我意谓的吗被受到的影响宝贝紧紧地捉住我我想念你，想要吻你 Everytime 我见到你的脸我将等候你的宝贝每个和每天的我爱你我想要你你是那一个我活的为而且我再不能轮流它我爱你我需要你我能做使你见到什么你是我的唯一一个你是我的唯一一个而且我知道资讯科技必须当我能做使你见到什么我的爱将会继续如此对我的心听而且知道你将会发现我的爱将会哪里去在我们之间的将来谎言男孩哦小孩我爱你如此我爱你我想要你你是那一个我活的为而且我再不能轮流它我爱你我需要你我能做使你见到什么你是我的唯一一个我爱你我想要你你是那一个我活的为而且我再不能轮流它我爱你我需要你我能做使你见到什么你是我的唯一一个

In violent emotions, one does not read, one flings to the earth, so to speak, the paper which one holds, one clutches it like a victim, one crushes it, one digs into it the nails of one's wrath, or of one's joy; one hastens to the end, one leaps to the beginning; attention is at fever heat; it takes up in the gross, as it were, the essential points; it seizes on one point, and the rest disappears.

人在感情强烈冲动时，是不能好好看下去的。他一把抓住手里的纸，可以说，当成俘虏似的全力揪住，捏作一团，把愤怒或狂喜的指甲掐了进去，一眼便跑到了末尾，又跳回到开头，他的注意力也在发高烧，他只能看懂一个大概，大致的情况，一些主要的东西，他抓住一点，其余部分全不见了。

This route overcomes the shortcoming on environmental pollution and poison in the cyanide process, which is widely applied in our country. This route isnt known in our country. It is a new environmental pollution process, and is in according with the progress of industrial policy. In the second step, the methyl isobutyl ketone which introduced in the patent is replaced by the material A, and the marketable foreground of the byproduct is considered. It is an innovated process, which can supply a gap in our county.

文中探讨的工艺路线克服了国内所广泛采用的氰化法路线所带来的环境污染和人体危害问题，目前国内尚未有报道，是一条新型的环保路线，符合国家产业政策的发展方向，而且在第二步3-氨基丙醇的合成中，用原料A取代专利中介绍的甲基异了酮，在获得产物的同时又兼顾了副产物的市场潜力，工艺上具有创新性，可填补国内该工艺路线的空白。

I hope that this success is just the first in a long series.

我希望这只是成功的开始。

Three dimensional stresses on the interface of tire and soil, tire deflection and soil sinkage are measured.

测试轮胎——沙土界面上的三向应力、轮胎变形和沙土沉陷；建立考虑剪切应力对沙土承载特性产生影响的车轮牵引性能预测模型；分析车轮作用下土壤的纵向稳定性。