查询词典 not

To prepare recombinant fox growth hormone, we amplified its cDNA from silver fox pituitary tissue by RT-PCR and cloned into yeast shuttle vector pPIC9K down stream of α-factor signal peptide sequence by SnaB I and Not I restriction sites. The recombinant secretion vector pPIC9K/fGH, linearized by Sal I, was transformed into histidine-deficient Pichia pastoris strain GS115 by electroporation.

为制备重组狐狸生长激素，采用RT-PCR方法，从银狐垂体中扩增fGH cDNA基因，利用SnaB I和Not I位点将fGH基因插入到酵母分泌型表达载体pPIC9K中α-因子信号肽的下游，构建成fGH基因的酵母分泌型表达载体pPIC9K/fGH，载体经Sal I酶切线性化后，通过电转移将线性化的pPIC9K/fGH转化到组氨酸缺陷型酵母宿主菌GS115中。

Methods Amplify IL-6 (23)-PE40 gene from the previously constructed recombinant plasmid pKK-IL-6(23)-PE40 by PCR, and insert to the SnaB Ⅰand NotⅠ sites of vector pPIC9. Transform the constructed recombinant plasmid pPIC0-SN to P. pastoris GS115 by electroporation, screen Mut-recombinants for expression under induction of methanol.

采用PCR技术，将目的基因IL-6(23)-PE40插入到pPIC9载体中SnaBⅠ与NotⅠ位点，电转化至巴斯德毕赤酵母GS115中，筛选Mut-型重组酵母；甲醇诱导表达，体外细胞试验检测其细胞毒性。

And when an Englishman says that something is not cricket , the allusion is also to a game that is by no means universal in the English-speaking countries.

当英国人说& something is not cricket &时，这个引喻是指英国特有而在其他英语国家里不一定流行的板球赛。

He will stick at nothing ,not even breaking the law, to get his own way.

问题补充：甚至是破坏法律，为什么还要用not呢？

The beast has gone away to the landfill now, but you can still hear the clacking and clattering of its rickety skeleton in songs like "Not Your Lover" and "Echo".

那个破钢琴现在可能已经成垃圾了，但你却可以在《Not Your Lover》和《Echo》等几首歌里，感受到它叮叮咚咚的声响和摇摇晃晃的身躯。

The fact that most of these young people have go to Europeanized or Americanized societies makes it unlikely that they will hold as tightly to the value of duty as they should have if they had not left their countries

在做高考阅读理解题时，要注意选项中only，not…at all，absolutely等表示绝对意义的词的使用，含有这类词的选项往往因其意义过于绝对化而与原文中所表达的相关内容不一致。

……The fact that most of these young people have go to Europeanized or Americanized societies makes it unlikely that they will hold as tightly to the of duty as they should have if they had not left their countries

在做高考阅读理解题时，要注意选项中 only ， not…at all ， absolutely 等表示绝对意义的词的使用，含有这类词的选项往往因其意义过于绝对化而与原文中所表达的相关内容不一致。

Traditional GO/NOT GO thread gaging techniques are time consuming, produce excessive gage wear, and require constant maintenance and calibration.

传统的 GO/NOT GO 螺纹规技术需要花费大量的时间，产生过度的量规磨损并且要求连续不断的维护与校准。

If you liked this story and would like to get your hands on a horseshoeing method that has a 16 year 100% track record Not One Single Lame Horse rush and click the website link down below in the Author Bio do it before you and your horse loose out.

如果您喜欢这个故事并希望得到您的双手就一horseshoeing的方法，有一个16年的100 ％往绩 not单一跛脚horse 繁忙，并点击网站链接下来，在下面的作者生物这样做之前，您和您的马松散。

Breakfast is not included in the price and costs 31,00 per person.Room service incurs an extra charge.

如果您选择并预订的房间已标明是&预缴/不予发还（PREPAID/NOT REFUNDABLE）&的房型。

If you are unfortunate enough to the lovelorn, please tell me, I will help you out, really, please contact me!

如果你不幸失恋了，请告诉我，我会帮助你摆脱困境，真的，请联系我啦！

China's plan to cut energy intensity by 20 percent and pollutant discharges by 10 percent between 2006 and 2010 is a case in point.

中国计划在2006年到2010间降低20%的能源强度和减少10%的主要污染物排放，就是一个这样的例子。