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It only grows harder, only grows hotter He blows us all over these hoes is all on him Coast to coast shows, he''''s know as the globetrotter Lonely roads, God only knows He''''s grown farther from home, he''''s no father He goes home and barely knows his own daughter But hold your nose cuz here goes the cold water His bosses don''''t want him no mo, he''''s cold product They moved on to the next schmoe who flows He nose dove and sold nada So the soap opera is told and unfolds I suppose it''''s old potna, but the beat goes on Da da dum da dum da da You better lose yourself in the music, the moment You own it, you better never let it go You only get one shot, do not miss your chance to blow This opportunity comes once in a lifetime yo You better lose yourself in the music, the moment You own it, you better never let it go You only get one shot, do not miss your chance to blow This opportunity comes once in a lifetime yo No more games, I''''ma change what you call rage Tear this motha****in roof off like 2 dogs caged I was playin in the beginnin, the mood all changed I been chewed up and spit out and booed off stage But I kept rhymin and stepwritin the next cypher Best believe somebody''''s payin the pied piper All the pain inside amplified by the fact That I can''''t get by with my 9 to 5 And I can''''t provide the right type of life for my family Cuz man, these goddam food stamps don''''t buy diapers And it''''s no movie, there''''s no Mekhi Phifer, this is my life And these times are so hard and it''''s getting even harder Tryin to feed and water my seed, plus See dishonor caught up bein a father and a prima donna Baby mama drama''''s screamin on and Too much for me to wanna Stay in one spot, another jam or not Has gotten me to the point, I''''m like a snail I''''ve got to formulate a plot fore I end up in jail or shot Success is my only motha****in option, failure''''s not Mom, I love you, but this trail has got to go I cannot grow old in Salem''''s lot So here I go is my shot.

它只变比较难，只变比较热他在这些之上吹我们全部引人注意在他身上是全部沿海岸而行沿海岸而行表演，他''''s 知道如世界观光旅行家孤单的道路，上帝只知道他''''s 长大的更远从家，他''''s 没有父亲他回家而且几乎不能认识他自己的女儿但是支撑这里的你的鼻子 cuz 去冷水他的老板君''''t 想要他没有 mo，他''''s 寒冷产品他们转移至流动的下 schmoe 他嗅到鸽子而且卖 nada 因此连续剧被告诉而且展开我推想它''''s 旧的 potna，但是打继续 Da da dum da dum da da 你最好在音乐方面失去你自己，片刻你拥有它，你更不再让它去你只拿注射，不失踪你的机会吹这一个机会在终生的 yo 中被提出一次你最好在音乐方面失去你自己，片刻你拥有它，你更不再让它去你只拿注射，不失踪你的机会吹这一个机会在终生的 yo 中被提出一次没有较多的游戏，我''''妈变化什么你呼叫发怒流泪这 motha****在屋顶中走开相似的 2只狗关进笼内我在 beginnin 中是 playin，心情全部改变了我被嚼碎和唾液出自和离开阶段发出嘘声但是我保持了 rhymin 和 stepwritin 下一个零最好相信某人''''s payin 斑驳的吹笛者所有的痛苦内部藉着事实扩大了我能''''t 经过由于我的 9 到 5 而且我能''''t 为我的家庭提供正确类型的生活 Cuz 男人，这些 goddam 食物邮票君''''t 买尿布而且它''''s 没有电影，在那里'''' s 没有 Mekhi Phifer，这是我的生活而且这些时代如此难和它''''s 变得平坦比较难 Tryin 喂而且加水给我的种子，加号见到被追赶 bein 一位父亲和主要的 donna 的不名誉纵容妈妈戏剧''''s screamin 在和之上太很多的我而无法想要到留在一个地点，另外的一罐果酱已经扼要得到我，我''''m 喜欢一个蜗牛我''''ve 开始制定一个情节在前的我在监牢或注射中结束成功是我的唯一的 motha****在选项，失败''''s 不妈妈，我爱你，但是这一条踪迹已经开始去我不能够在沙连'''变旧's 运气因此我去在这里了是我的注射。

That I ought to consider I had been fed even by Miracle, even as great as that of feeding Elijah by Ravens; nay, by a long Series of Miracles, and that I could hardly have nam'd a Place in the unhabitable Part of the World where I could have been cast more to my Advantage: A Place, where as I had no Society, which was my Affliction on one Hand, so I found no ravenous Beast, no furious Wolves or Tygers to threaten my Life, no venomous Creatures or poisonous, which I might feed On to my Hurt, no Savages to murther and devour me.

我感到，我是被奇迹养活着，这种奇迹是罕见的，就像以利亚被乌鸦养活一样。应该说，正是由于发生了一系列的奇迹，我至今还能活着。在世界上所有荒无人烟的地区，我感到没有一个地方会比我现在流落的荒岛更好了。虽说这儿远离人世，形单影只，使我非常苦恼，但这儿没有吃人的野兽，没有凶猛的虎狼害我性命，没有毒人的动物和植物，吃下去会把我毒死，更没有野人会把我杀了吃掉。

And see no end to the landscape,new objects presenting themselves as we advan ce;so,in the commencement of life,we set no bounds to our inclinations.nor to the unrestricted opportunities of grastifying them.we have as yet found no obs tacle,no disposition to flag;and it seems that we can go on so forever.we look round in a new world,full of life,and motion,and ceaseless progress;and feel in ourselves all the vigour and spirit to keep pace with it,and do not foresee from any present symptoms how we shall be left behind in the natural course o f things,decline into old age,and drop into the grave.it is the simplicity,and as it were abstractedness of our feelings in youth,thatidentifie s us with nature ,and(our experience being slight and our passions strong)delu des us into a belief of being immortal like it.our short-lives connexion with existence we fondly flatter ourselves,is an indissoluble and lasting union-a h oneymoon that knows neither coldness,jar,nor separation.as infants smile and s leep,we are rocked in the cradle of our wayward fancies,and lulled into securi ty by the roar of the universe around us0we quaff the cup of life with eager h aste without draining it,instead of which it only overflows the more-objects p ress around us,filling the mind with their magnitude and with the strong of de sires that wait upon them,so that we have no room for the thoughts of death.

此时，但觉好风光应接不暇，而且，前程更有美不胜收的新鲜景致。在这生活的开端，我们听任自己的志趣驰骋，放手给它们一切满足的机会。到此为止，我们还没有碰上过什么障碍，也没有感觉到什么疲惫，因此觉得还可以一直这样向前走去，直到永远。我们看到四周一派新天地——生机盎然，变动不居，日新月异；我们觉得自己活力充盈，精神饱满，可与宇宙并驾齐驱。而且，眼前也无任何迹象可以证明，在大自然的发展过程中，我们自己也会落伍，衰老，进入坟墓。由于年轻人天真单纯，可以说是茫然无知，因而将自己跟大自然划上等号；并且，由于经验少而感情盛，误以为自己也能和大自然一样永世长存。我们一厢情愿，痴心妄想，竟把自己在世上的暂时栖身，当作千古不变、万事长存的结合，好像没有冷淡、争执、离别的密月。像婴儿带着微笑入睡，我们躺在用自己编织成的摇篮里，让大千世界的万籁之声催哄我们安然入梦；我们急切切，兴冲冲地畅饮生命之杯，怎么也不会饮干，反而好像永远是满满欲溢；森罗万象纷至沓来，各种欲望随之而生，使我们腾不出工夫想死亡。

If what the girl grows is beautiful, you are direct boast she is beautiful, although her front is pie-eyed, you are OK still boast her flank, end face, underside, or a certain angle, it is rear and beautiful even, if 361 degrees pie-eyed, you say him a certain expression, a certain instant is beautiful, in any time like fructification any angle did not have a law to bear that you also do not abandon, more not strong praise she is beautiful, seem to be in mock other people in that way, you can say her figure is good, or at least one part figure is very good, the bosom is good say the bosom is good, the bosom is no good say a small of the back, the waist is no good say buttock, buttock is no good say a leg, the leg is no good say arm, arm is no good say a hand,"Your hand look is very good ah ", leng Buding cast going out also have power one sentence very much so, if these parts accident is differ in what outside going out, enter is honest do not have the mouth below the law, that or can undertake eulogizing with respect to adjunct of a few surface layer, e.g.,"It is really good that your hair pledges, inherent, follow your father?"

假如女孩长的漂亮，你就直接夸她漂亮，即使她正面不漂亮，你还可以夸她侧面，顶面，底面，或者某个角度，甚至是背面漂亮，假如三百六十一度都不漂亮，你就说他某个表情，某个瞬间漂亮，假如实在任何时间任何角度都没法忍了那你也不要放弃，更不要硬夸她漂亮，那样似乎在讽刺人家，你可以说她身材好啊，或者最少一部分身材很好，胸好就说胸好，胸不行说腰，腰不行说臀，臀不行说腿，腿不行说胳膊，胳膊不行说手，&你手形很不错啊&，冷不丁的抛出往这么一句也是很有杀伤力的，假如这些部分都是非不一里出外进的实在没法下嘴，那还是可以就一些表层附属物进行歌颂，比如说，&你发质真好，天生的吧，随你爸爸吧？&

In the future,network virtual community become more and more variously , more and more like reality.even better than reality for its excitiment and amusement.what realty have exist in fictity.what can not realise in realty is easy to do in fictity.you can marrige with your lover,you do business with your cooperation,you can speculation on stocks,you can make friends,you can do every thing you want unless some thing not allowed in law,which make the bound become more and more fuzzy betwen fictity and reality.people will mist easily between them for a long time.it is scareful to face such blurred phenomenon.so for those who have not enough ability to distinguish ,especiely youth and children,it is easy to lost themselves.

不久的将来网络虚拟社区会越来越全面。跟现实几乎一模一样，甚至比现实的东西还要全面刺激。现实中有的虚拟空间里都有，现实中无法实现的可以在社区里得到满足和实现。可以在虚拟社区里结婚生子，做生意，炒股票，交朋友等等。这样虚拟和现实的界限会越来越模糊，时间久了人们就会在两者之间迷离了，形成虚拟和现实的交错现象。有时候想想也挺可怕的。所以对于没有做够判断和分辨能力的人来说，特别是青少年和儿童，真的容易迷失自己。

Obviously, the optimization management in middle-sized or small cities of China is very necessary. The basic characteristics of municipal solid waste generated in middle-sized or small cities of China are put forward in the paper. The total amount of municipal solid waste generated in middle-sized or small cities is significantly less than that in large cities. The amount of garbage is about 1 kg per capita and year. In the garbage, inorganic material is more than organic material, the combustible less than incombustible and the amount of uncompostable garbage more than the amount of garbage that can be disposed for composting. The value of heat of garbage is about 800 kal/kg.

通过总结，我国中小城市生活垃圾的基本特点为：各城市的生活垃圾产生量相对较小，人均生活垃圾产生量大约在1kg/d左右；垃圾组分中以煤渣、灰土为主的无机成分含量过高，可回收物质成分含量很低，不可燃成分多于可燃成分，不可堆腐成分多于可堆腐成分，垃圾热值大约在800kal/kg左右；并且在我国，南方城市与北方城市，经济发达城市与欠发达城市之间的生活垃圾产生量与组成也存在着明显的地区差异。

These results show that:(1) the results retrieved from the Abel integral and absolute TEC inversion are consistent on the whole at higher orbits altitude (~800 km), and in good agreement with those measured by ionosondes; the results retrieved from absolute TEC inversion are in better agreement with those obtained from ionosondes than that by the Abel integral inversion at lower orbits attitude (~500 km);(2) the maximal electron density N m from absolute TEC inversion is closer to N mF2 from ionosondes than that from Abel integral inversion, and the method of the former is more rigorous and effective than that of the latter;(3) absolute TEC is more sensitive to cycle slip than Abel integral, but for the two ways significant loss of inversion precision due to cycle slip always exists.

结果表明：（1）在较高轨道高度（约800 km），Abel积分与绝对TEC方法的反演结果基本一致，都与电离层测高仪反演结果符合良好；在较低轨道高度（约500 km），绝对TEC反演精度优于Abel积分反演精度；（2）绝对TEC反演的最大电子密度 N m较Abel积分法获得的结果更接近于电离层测高获得的峰值电子密度 N mF2，绝对TEC反演法更加严密和有效；（3）周跳对绝对TEC反演结果的影响较Abel积分反演结果的影响更为敏感，但无论哪种方法，周跳对反演精度都造成严重损失。

The results showed that the American shad fingerlings stopped opercular movement less than half an hour after anesthetia when the fish were exposed to concentration of MS-222 at 75mg/L, clove oil at 20mg/L and bezocaine at 40mg/L. The fish recovered to normal behaviors again after exposed in air for less than 10min when transferred into fresh water. The shad fingerlings had good anaesthesia for more than 12h at 20-30mg MS-222/L, 8-10mg/L clove oil and 20-30mg/L benzocaine. The anesthetic effects were enhanced at high water temperature and changed with fish sizes. Small fish were more easily in good anaesthesia than the bigger one in MS-222 while the opposite case was found in clove oil and in benzocaine.

麻醉试验结果表明：在较高麻醉浓度（MS-222为75mg/L以上，丁香油为20mg/L以上，苯唑卡因为40mg/L以上）下，鱼很快（30min内）停止鳃盖张合运动，且停止鳃盖运动的鱼在空气中暴露一定时间（10min内）后也能够复苏；在适宜的麻醉浓度（Ms-222为20~30mg/L，丁香油为8~10mg/L；苯唑卡因为20~30mg/L）下，鱼能够进入麻醉状态，且能保持很长时间(12h)；麻醉效果随着水温的升高而增强；在20mg/L MS-222麻醉剂下，小规格鱼较大规格鱼更容易进入麻醉状态，而在10mg/L丁香油和20mg/L苯唑卡因麻醉剂下，小规格鱼却难进入麻醉状态。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

It was verified that the particle size of more than 90% of the acidic phosphorite powder was less than 4.227 μm, and more than 90% of activated phosphorite powder was less than 220 nm.

采用扫描电镜观察、X－射线衍射分析、激光粒度测试，证明了90%的酸化磷矿粉粒径为4.227 μm，90%的活化磷矿粉粒径达到220 nm。

It has to be computed by the host for the actual clock rate.

主控制器可以用来计算实际的时钟速率。

Let's see what you got.

让我看看你的东西