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Methods: Fresh saphena rings were sudied in organ chamber after incubating with Krebs-Henseleit bicarbonate solutions, FWM, KH with 0.1mmol/L NCR, FWM with 0.1mmol/L NCR at 37℃ for 1 hour. Precontraction induced by prostaglandin F2 (U4661930nmol/L) and endothelium-dependent relaxation induced by calcium ionophore A23187 l0^(-10~l0^(-6) mol/L were measured in the presence of indomethacin (7μmol/L) and LNNA (300μmol/L). Result: No significant changes were found in the 4 groups under the light microscope.

采用器官槽法研究在37℃有氧条件下用重碳酸盐缓冲液、含0.1mmol/L NCR的KH液、FWM液、含0.1mmol/L NCR的FWM液中浸泡血管环1h后，再用7μmol/L吲哚美辛和300 μmol/L N-硝基－L-精氨酸作用后，检测30 nmol/L U46619及不同浓度A23187引发的血管收缩舒张反应。

Collector current is proportional to concentration of dopamine in the range over 5.0×10〓mol/L to 5.0× 10〓mol/L and remains independent of ascorbic acid concentration to approximately 5. 0×10〓mol/L.

由于在收集电极上消除了由电位变化引起的充电电流和阴极-阳极工作时的反馈作用，多巴胺的检测限可达7.5×10〓mol/L，线性范围为5.0×10〓～5.0×10〓mol/L。

The results show that melting point and fusion enthalpy of the fresh nano-aluminium powders are 649.7 ℃ and 3.7 kJ/mol in Ar, respectively, which are 10.3 ℃ and 7.09 kJ/mol lower than those of coarse-grained Al.

研究结果表明：在Ar气流中，新纳米铝粉的熔点为649.7 ℃，熔化焓为3.7 kJ/mol，分别比粗晶铝的熔点(660 ℃)和熔化焓(10.79 kJ/mol)低10.3 ℃和7.09 kJ/mol；在N2气流中，从20 ℃升温到800 ℃时，新纳米铝粉增重约为33%，熔点为648.8 ℃。

A linear relationship between peak current and concentration was obtained in the range from 1×10^(-7)~2×10^(-5) mol/L, and the detection limit (signal to noise=3) is 8×10^(-9)moL/L after 4 min open-circuit accumulation.

在1×10^(-7)~2×10^(-5)mol/L浓度范围内，峰电流与浓度呈良好的线性关系，开路富集4 min后，检出限（3倍信噪比）为8×10^(-9)mol/L.2×10^10(-5)mol/L×2-硝基苯酚平行测定8次的相对标准偏差为3.8%。

Rate constants of carbon dioxide radical anion〓 reacting with 5,5dimethyl-1-pyrroline-N-oxide、1-naphthoquinone and N,N,N,N-tetramethyl 1,4 -phenylene diamine were determined by detecting the changes of radical concentrations with time, and they were 5.5×10〓mol���Ls〓、4.5×10〓mol���Ls〓 and 6.7×10〓nol〓Ls〓 respectively.

测定了在含〓的CdS胶体体系中〓与5，5-二甲基-1-吡咯氮氧化物、1-萘醌和N，N，N，N-四甲基对苯二胺正离子自由基反应的速率常数，它们分别为5.5×10〓mol〓Ls〓、4.5×l0〓mol〓Ls〓和6.7×10〓mol〓Ls〓。

By pretreatment of picrotoxin (30 μmol/L) and strychnine (1.0 μmol/L), inhibitory amino acid receptor antagonists, the potentiation of cVLF-EPSPs by TRH was kept effective in 3 MNs.

使用印防己毒素(30μmol/L)和士的宁(1.0μmol/L)阻断抑制性氨基酸受体后，灌流TRH(0.2μmol/L)15min仍然在3个MN呈现了易化cVLF-EPSP的作用。

From the potential energy profile, it can be predicted that, the dominant channel of this reaction consists of three steps: the two reactants first form a three-membered ring intermediate (INT1) through a barrier-free exothermic reaction of 19.2 kJ/mol; three-membered ring intermediate (INT1) isomerizes to a four-membered ring silylene(P2) via the transition state (TS2) with an energy barrier of 22.8 kJ/mol; four-membered ring silylene(P2) further reacts with ethene(R2) to form a silicic bis-heterocyclic compound (P3), which is also a barrier-free exothermic reaction of 132.2 kJ/mol.

用CCSD//MP2/6-31G*方法研究了单重态二甲基亚烷基硅烯与乙烯生成硅杂双环化合物环加成反应的机理，根据该反应的势能面可以预言，该反应的主反应通道有三步组成：第一步是两反应物（R1， R2）首先生成了一三元环中间体（INT1），它是一无势垒的放热反应，放出的能量为19.2 kJ/mol；第二步是三元环中间体(INT1)经过渡态TS2异构化为了四元环硅烯（P2），其势垒为22.8 kJ/mol；第三步是四元环硅烯（P2）进一步与乙烯(R2)反应生成了硅杂双环化合物（P3），该反应也是一无势垒的放热反应，放出的能量为132.2 kJ/mol。

This equation was tested by the renaturation processes of denatured egg white lysozyme in guanidine hydrochloride and urea solutions, with the results to show that when guanidine hydrochloride and urea concentrations were separately higher than 1.25 and 3.00 mol/L or separately lower than 1.00 and 3.00 mol/L, the refolding intermediates of egg white lysozymes were more easily aggregated to aggregate state or more easily renatured to native state, respectively.

以蛋白溶菌酶在盐酸胍和脲溶液中的复性过程对此关系进行了验证，结果表明，当溶液中盐酸胍和脲浓度分别大于1.25 mol/l和3.0 mol/l或分别小于1.0 mol/l和3.0 mol/l时，它们的折叠中间体分子分别更倾向于集聚成集聚体或更倾向于复性成原始态分子。

Results were shown as followings:(1) Sodium selenite at 0～2.5 μmol/L significantly increased the antioxidative capacity of L-02 cells without having remarkable impact on SMMC-7721 cells;(2) Sodium selenite at concentrations above significantly increased telomerase activity, hTERT gene expression and telomere length of L-02 cells without significant impact on SMMC-7721 cells;(3) Sodium selenite at higher concentrations (larger than 5 μmol/L) resulted in peroxidation of L-02 cells, while scutellarin significantly counteracted its effect;(4) Selenium-rich amino acids from silkworm pupas in the range of 0.5～2.5 μmol/L Se significantly inhibited SMMC-7721 cell growth, induced apoptosis and cell cycle change, and the generation of reactive oxygen species. In contrast, sodium selenite and selenomethionine only had weak impact on them at the same concentrations;(5) A new selenium-containing protein was found from selenium-rich silkworm pupas, which is worthy to be study further;(6) An expression vector containing ansense RNA of hTERT gene were constructed and used to transfect SMMC-7721 cells. They were observed to inhibit hepatoma cells.

结果如下：（1）0～2.5μmol/L亚硒酸钠显著性增强L-02细胞的抗氧化能力；而对SMMC-7721细胞的作用不显著；（2）该浓度硒显著性提高L-02细胞端粒酶活性、增强hTERT基因表达和延长细胞端粒长度；而对SMMC-7721细胞的作用均不显著；（3）高浓度硒（5μmol/L以上）显著性抑制L-02细胞生长、致细胞过氧化，灯盏花素能拮抗硒所致过氧化、降低硒毒性；（4）0.5～2.5μmol/L富硒蚕蛹氨基酸显著性抑制肝癌细胞SMMC-7721生长、导致细胞凋亡和周期改变、诱导细胞产生活性氧，同浓度亚硒酸钠和硒代蛋氨酸对其抑制不显著；（5）富硒蚕蛹蛋白经分离纯化和鉴定后发现存在一新含硒蛋白，其结构和功能有待研究；（6）通过已有的含hTERT基因质粒，成功构建hTERT反义RNA表达质粒，转染SMMC-7721细胞后对其生长具有抑制作用。

Japonicus.Nerve extraction substance of sea star and dithiothreitol has been reported to be effectively to induce oocyte maturation in sea cucumbers. In this study we tested their effects on oocyte maturation induction in sea cucumber A. japonicus. The results are as follows, oocyte of A. japonicus does not get mature spontaneously. Neither NES alone nor NES with follicle suspension can induce oocyte maturation. While DTT can prominently increase the percentages of germinal vesicle break down. When the concentration of DTT is between10-1 mol/L and 10-3 mol/L, the effect of maturation induction is more sufficient. When the concentration is about 10-2 mol/L, the percentage of GVBD can reach up to more than 90%.

分别利用在海参中有诱导作用的海星神经提取液和二硫苏糖醇对从成熟卵巢解剖出的刺参卵母细胞进行诱导成熟试验，结果显示，刺参的卵母细胞在海水中不会发生自发成熟，海星神经提取液以及神经提取液加滤泡悬浮液对卵母细胞没有诱导成熟的作用，而二硫苏糖醇处理可以诱导卵母细胞生发泡破裂，当DTT溶液的浓度处于10-1 mol/L到10-3 mol/L之间时，对刺参卵母细胞的促熟作用较为明显，在10-2 mol/L左右时，卵母细胞的诱导成熟率可以达到90%以上。

If you are unfortunate enough to the lovelorn, please tell me, I will help you out, really, please contact me!

如果你不幸失恋了，请告诉我，我会帮助你摆脱困境，真的，请联系我啦！

China's plan to cut energy intensity by 20 percent and pollutant discharges by 10 percent between 2006 and 2010 is a case in point.

中国计划在2006年到2010间降低20%的能源强度和减少10%的主要污染物排放，就是一个这样的例子。