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Rats in the modeling exercise group were fed with ethanol and pinguid food by intragastric gavage, undergoing treadmill exercise at a speed of 25 m/min for 4 weeks, with the slope of 15% and the frequency of 30 min/d.

造模运动组：给予乙醇＋高脂饮食灌胃，进行跑台运动训练，跑速25 m/min，15%坡度，30 min/d。运动持续4周。

And the amplification procedure was as follows: preparatorily denaturing for 3 min at 94℃, denaturing for 30 sec at 94℃, annealing for 30 sec at 37℃, elongating for 90 sec at 72℃, 41 cycles, and elongating for 7 min at 72℃.

扩增程序为：94℃预变性3 min，然后于94℃变性30 s，37℃复性30 s，72℃延伸90 s，循环41次，最后于72℃延伸7 min。

The reseach on activity changes of SOD, POD and CAT during the somatic embryogenesis of Y35 showed:(1) The activty of SOD was from 52.98 to 133.20 U·g-1·h-1, and remained a rising trend after early single embryo forming, this revealed that SOD might be positively correlated to the differentiation of embryogenic cell and the development of somatic embryo.(2) The activty of POD was from 0.05 to 0.50 U·mg-1·min-1, ascended firstly and desceded later, and was highest in embryogenic callus and lowest in late single embryo , this revealed that POD might be positively correlated to the division and differentiation of proembryo mass, while negatively correlated to the development of PEMⅢto late single embryo.(3) The activty of CAT was from 0.86 to 2.81 U·mg-1·min-1, showed an up-down-up trend, reaching to the highest peak at the time of early embryo formating and decreasing to the lowest at the time of early cotyledonary embryo formating, this revealed that CAT might be positively correlated to the development of early single embryo, while negatively correlated to the formation of middle single embryo and early cotyledonary embryo.The changes in activty of SOD, POD and CAT indicated these three antioxidant enzymes coregulated the differentiation and development of embryogenic cells during Larix somatic embryogenesis.4. Differentially expressed cDNA libraries of the stages of proembryo mass and somatic embryo maturation were successfully constructed by suppression subtractive hybridization.

对Y35体细胞胚胎发生过程中抗氧化酶活性变化的研究显示：(1)SOD活性在52.98~133.20 U·g-1·h-1之间，并在早期单胚形成后一直保持上升的趋势，表明其与胚性细胞的分化及体细胞胚的发育均具呈正相关；(2)POD活性在0.05~0.50 U·mg-1·min-1之间，呈现出先下降后升高的趋势，在胚性愈伤组织中最高，而在后期单胚形成时降至最低，表明其与原胚团的分裂和分化呈正相关，但与PEMⅢ向后期单胚的发育呈负相关；(3)CAT活性在0.86~2.81 U·mg-1·min-1之间，表现出升-降-升的变化趋势，在早期单胚形成时升至最高，在早期子叶胚形成时降至最低，表明其与早期单胚的发育呈正相关，而与中期单胚和早期子叶胚的发育呈负相关。

Results By using two-phase solvent systems composed of petroleum ether-ethylacetate-methanol-water (22:25:23:17) at the flow rate of 2.0 mL/min, with rotating speed of 800 r/min, and at temperature of 25℃, the yield of d1-tetrahydropalmatine and of protopine purified from the total alkaloids being 16.9% and 14.7%, with purity of 99.3% and 98.8% respectively can be reached.

结果 石油醚－乙酸乙酯－甲醇－水(22：25：23：17)为溶剂体系，流速为2.0 mL/min，仪器转速800 r/min，温度25℃，从延胡索总碱中可一步纯化得到原阿片碱和延胡索乙素，得率分别为16.9%和14.7%，纯度分别为99.3%和98.8%。

The maximal reaction rate of pyrotechnical reagent with potassium nitrate added with CuO by mixing method is 7.38 min-1, which is 1.30 times of that without catalyst. The time to maximal rate is 53.55 min, which is lower than that without catalyst by 66.3%.

氧化铜对含硝酸钾的烟火药剂具有催化作用，混合法添加的催化效果最佳；此药剂最大反应速率为7.38 min-1，是不含催化剂药剂的1.30倍；到达最大反应速率时间为53.55 min，比不含催化剂的药剂降低了66.3%。

The maximal reaction rate of pyrotechnical reagent with potassium perchlorate added with Fe2O3 by crystallization method is 8.10 min-1, which is 4.09 times of that without catalyst. The time to maximal rate is 8.52 min, which is lower than that without catalyst by 92.8%. It was also found that CuO can catalyze pyrotechnical reagent with potassium nitrate, and the addition is better with mixing method.

结果表明，氧化铁对含高氯酸钾的烟火药剂具有催化作用，以结晶添加法催化效果最佳；此药剂的最大反应速率为8.10 min-1，是不含催化剂药剂的4.09倍；到达最大反应速率时间为8.52 min，比不含催化剂的药剂降低了92.8%。

METHODS Rats were pretreated with rutaecarpine 10 min before the experiment, and then the left main coronary artery of rat hearts was subjected to 60-min occlusion followed by 3h reperfusion.

大鼠在实验前10 min静脉注射吴茱萸次碱，然后结扎冠状动脉左前降支60 min，再灌注3 h。

Normal heart rate determined by the rhythm of the sinoatrial node, resting adult is about 60 ~ 100 times/min, an average of about 75 times/min.

正常心率决定于窦房结的节律性，成人静息时约60～100 次/ min ，平均约75次/ min。

Urine samples were first precipitated with trichloroacetic acid, then centrifuged at 12000 r/min for 15 min at 4 ℃, and finally added with NaOH to neutralize the supernatant.

尿标本经三氯乙酸处理，在4 ℃下，以12000 r/min的速率离心15 min，上清液用碱中和后，取30 μL直接进样。

A form ula was employed to calculate the heating time of die,it was 26 min which was close to the experiment heating time of 27 min.

计算出模具的加热时间为26 min，与实际加热时间27 min相近。

Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

万寿菊对土壤要求不严，以肥沃。排水良好的砂质壤土为好。

He unstepped the mast and furled the sail and tied it.

他拔下桅杆，把帆卷起，系住。