查询词典 messenger RNA

Methods: One-step trizol had used to get the total RNA of the four animal brain, then PEGS000 to purify the small RNA from the total RNA, after the ligation ofpoly on the 3\' and the adaptor on the 5\', the oligo primer will be used to reverse translation the small RNA, and then used the specific primers to amplification the small RNA sequences. After the gel purition recovery, the small RNA will be ligated to the T-vector, and be transformed to the TOP 10 E. coli, at last, we picked up the masc. clones for sequencing.

通过Trizol一步法提取四种动物脑中的总RNA，接着用PEG纯化其中的小分子RNA，再经过poly连尾反应在小RNA的3'端连接上一段poly，然后在5'端连上adaptor，用Oligoprimer将连接好的小RNA进行反转录，用根据adaptor和Oligoprimer设计的引物来扩增小RNA，扩增后的小RNA进行胶回收后连接到T载体上，转化TOP10大肠杆菌后挑取阳性克隆进行测序，得到的序列再经过生物信息学的分析后确定是否为miRNA。

RNA interference represents a common biological phenomenon of double-stranded RNA mediated post-transcriptional gene silencing, which lies in altitude plants and animals. The mechanism of this process is described as that: the dsRNA in cells are cut into double-stranded small interfering RNA. By joining with an effector complex termed RISC (RNA-induced silencing complex), siRNA binds to the cellular RNA with homologous sequences, and contributes to the degradation of the corresponding mRNA, the inhibition of specific gene expression.

RNAi技术是双链RNA(double strand RNA，dsRNA)介导的序列特异性的转录后基因沉默，广泛存在于高等植物和动物中，其机制是细胞内的dsRNA被Dicer剪切成双链的小分子干涉RNA (small interfering RNA，siRNA)与RNA诱导的基因沉默复合物结合，降解与之互补的靶mRNA，特异性抑制靶基因表达。

At present it is very difficult to predict all the RNA secondary structures. This paper describes RNA secondary structure prediction methods from 1970\' to this day in a systematic way some. Tracing back and following the tracks of prediction methods the author made a summary of RNA research progress and situation while a enumeration problem of RNA secondary structure is studied with the method of combinatorial enumeration.

目前所有有关RNA二级结构的预测算法要预测出所有序列的二级结构仍然很困难，本文系统介绍了从上世纪70年代以来发展至今的各类RNA二级结构预测算法，从对RNA结构预测方法的追溯和跟踪，对当今RNA研究的进展和形势做了一个总结，并且应用组合计数的技巧解决了一类RNA二级结构的计数问题。

The total RNA from brain tissue of substantia nigra of PD rats is extracted at the 1st, 2nd, 4th and 8th week and comparison is made between it and that from normal rats. The total RNA, after RNA formaldehyde degenerated sepharose electrophoresis and separation and Northern transfer, is adsorbed unto colloxylin membrane. Then ECL labeled Selenoprotein P and an unknown gene fragment are used as probes to hybridize with the RNA on the colloxylin membrane. And the hybridized result is obtained with enzyme-linked immunosorbent assay. Finally, analysis of OD is made using ONE-Dscan software.

分别提取1周、2周、1月、2月的帕金森大鼠（各8只）黑质区脑组织总RNA，并取相对应时间的正常大鼠的脑组织总RNA作为对照，经RNA甲醛变性琼脂糖凝胶电泳分离后，通过Northern转移，将其吸附在硝酸纤维素膜上，然后利用ECL标记的Selenoprotein P和一段未知基因为探针，与硝酸纤维素膜上的RNA分子进行杂交，再通过显影取得杂交的结果，应用ONE-Dscan软件进行光密度值分析。

The final product of transcription is then spliced and modified into one of three RNA molecules: messenger RNA, transfer, or ribosomal.

最后产品的转录然后剪接和修改分为三种 RNA 分子：信使 RNA

A sequence of three adjacent nucleotides in transfer RNA designating a specific amino acid that binds to a corresponding codon in messenger RNA during protein synthesis.

反密码子在蛋白质合成过程中，传送者RNA中的相关密码子与某种氨基酸结合，在传送RNA过程中出现的三个相邻的核□酸序列

In addition, the key molecules in protein synthesis process are amino acids, transfer RNA, messenger RNA, and ribosome.

从蛋白质资库中筛选出18个打结蛋白质，并针对这18个蛋白质打结的构形去推测其mRNA 模版构形。

Messenger RNA -- an RNA molecule that serves as a template for protein synthesis.

信使核糖核酸--核糖核酸的一种，当蛋白质合成中当作模板指定讯息的转译。

This type of RNA is called messenger RNA. The job of mRNA is to carry the message from the DNA to a ribosome, for translation of the gene into protein.

这种RNA被称为信使RNA。mRNA的工作是将DNA上的信息传递给核糖体，将基因上的遗传信息翻译为蛋白质。

Methods A nested RT-PCR was used for detecting one fragment of SARS-CoV RNA in oropharyngeal swabs from 3 SARS probable patients ,4 SARS suspect patients and other 27 patients with fever in Hangzhou, and the nested RT-PCR product from one SARS probable patient was sequenced.Meanwhile in these 3 SARS probable patients, other three RT-PCR methods, including a hemi -nested RT-PCR targeted for another fragment of SARS-CoV RNA, a real-time RT -PCR and a modified nested real-time RT-PCR, were employed to detect SARS-Co V RNA.Results Two positives were found in the 3 SARS pro bable patients, and none positive in 4 SARS suspect patients and other 27 patien ts with fever, using the nested RT-PCR.

方法对2003年杭州市3例严重急性呼吸综合征临床确诊患者、4例疑似和27名医学观察者的咽拭子标本用巢式实时RT-PCR检测SARS-CoV核酸，对阳性扩增产物进行核酸序列测定，同时对3例患者的标本应用半巢式RT-PCR检测另一位点SARS-CoV核酸片段，并应用常规实时RT-PCR技术及改良实时RT-PCR技术进行检测。

Warden: That's a beautiful dog, but don't bring her again.

那是条漂亮的狗，但是不要再把她带来了。

You get up in the morning – no hangover!

你第二天早上起来的时候—不会宿醉！