查询词典 mediated

The intralellular labeling methods developed for single cell analysis are summarized in this review, including cell itself acting as a microreaction chamber for derivatization, dericatization mediated by liposome or polyethylene glycol to increase permeabilization of cell membrane, on- and post-column derivatization during single cell analysis with capollary electrophoresis or microchip electrophoresis and labelling of cells with quantum dots.

本文综述了在单细胞分析中常用的荧光标记方法，包括细胞作为微反应器的衍生法，借助于脂质体与聚乙二醇等增加细胞膜通透性的衍生方法和在毛细管/芯片毛细管电泳分析单细胞时柱上衍生和柱后衍生法以及量子点的标记法等。

Higher efficiency of transgene on penggan and longan resulted significantly from carborundum wounding followed by coinfection with Agrobacterium-mediated transformation.

通过金刚沙创伤处理比未创伤处理的均获得了较多的Kan〓胚状体。从结果分析可见，金刚砂创伤对椪柑和龙眼转化效果的影响，均达到显著水平。

In the MVC design pattern,application flow is mediated by a centric Controller.

在MVC的设计模式下，应用的流向由中央控制器负责。

The advantage of supercritical carbon dioxide used as an environmental medium in polymerization and reactions in supercritical carbon dioxide are described. It includes homogeneous free radical polymerizations, precipitation free radical polymerizations, dispersion free radical polymerizations, emulsion and inverse emulsion free radical polymerizations, cationic polymerizations, ring-opening polymerization, melt-phase condensation polymerizations, sol-gel polymerizations, polymer blend synthesis, catalytic chain transfer polymerizations, oxidative coupling polymerization, nitroxide-mediated radical dispersion, atom-transfer radical polymerization, reversible addition-fragmentation chain transfer polymerization, electrochemical polymerization, simultaneous one-pot combination of enzymatic and chemical polymerization, copolymerization of carbon dioxide used as monomer.

评述了ScCO2作为聚合反应介质的优点，以及在ScCO2中可进行的聚合反应类型，包括均相聚合、沉淀聚合、分散聚合、乳液及反相乳液聚合、阳离子聚合、开环聚合、熔融态缩聚、溶液-凝胶聚合、聚合物混合合成、链催化转移聚合、氧化-偶合反应聚合、氮氧自由基可控活性聚合、原子转移自由基聚合、可逆加成-断裂链转移、电化学聚合、原子转移聚合与酶催化开环聚合两种活性聚合在ScCO2中同时聚合、二氧化碳作为原料共聚合等。

Tumefaciens mediated transformation using cotyledonary node as explants was optimized; 3 methods including cutting chapiter, ovary injection and chapiter spreading for pollen tube pathway was compared. Cutting chapiter and ovary injection can both achieve insect resistant gene tranformants.

优化了以子叶节为外植体的农杆菌介导转化技术；比较了切割柱头、子房注射和子房涂抹3种花粉管通道法处理授粉后黄瓜子房的转化效果，切割柱头和子房注射均可成功获得黄瓜抗虫转基因植株。

OBJECTIVE: To choice the optimal concentration of liposomes-mediated chemosynthesis siRNA transfection, and to discover a simple efficient RNA interference application.

目的：筛选脂质体介导化学合成siRNA转染原代心肌细胞的最佳浓度，以及简单高效的RNA干扰操作方法。

Our work will be useful for better understanding of the miRNA-mediated control of the chemotaxis and thermotaxis in C.

本研究将有助于我们更好的了解线虫中可能由miRNA调控的化学趋向性和温度趋向性行为。

Our work will be useful for better understanding of the miRNA-mediated control of the chemotaxis and thermotaxis in C. elegans .

本研究将有助于我们更好的了解线虫中可能由miRNA调控的化学趋向性和温度趋向性行为。

The following methods were used in the study, such as viral inoculation of animal, pathological method, flow cytometry, RT-PCR, in situ hybridization, immunohistochemistry and terminal deoxy nucleotidyl transferase mediated dUTP nick end labling . Morphological changes of the infected chickling, dynamic changes of T, B cells and T subsets, changes of adhesion molecules on the lymphocyte surface, necrosis and apoptosis of lymphocyte and neurons, changes of MIP-1β and IL-8 producing cells in the brain, cell types of perivascular tuffing in cerebral tissue were systematically studied.

在研究过程中，采用了病毒接种技术、普通病理学研究方法、流式细胞仪技术、RT-PCR技术、原位杂交技术、免疫组化技术、凋亡细胞末端标记技术，系统研究了不同日龄的SPF雏鸡人工感染AEV-NH937株后的病理变化，T和B淋巴细胞、T淋巴细胞亚群的动态变化规律，淋巴细胞表面某些粘附分子的变化，淋巴细胞和神经元的坏死与凋亡，雏鸡脑组织中产生趋化因子MIP-1β和IL-8细胞的变化规律，脑组织中围官性细胞浸润的细胞类型。

The following methods were used in the study, such as viral inoculation of animal, pathological method, flow cytometry, RT-PCR, in situ hybridization, immunohistochemistry and terminal deoxy nucleotidyl transferase mediated dUTP nick end labling. Morphological changes of the infected chickling, dynamic changes of T, B cells and T subsets, changes of adhesion molecules on the lymphocyte surface, necrosis and apoptosis of lymphocyte and neurons, changes of MIP-1β and IL-8 producing cells in the brain, cell types of perivascular tuffing in cerebral tissue were systematically studied. The key research results were.(1)The average percentage of CD19+cell in blood, bursa and spleen, after 3, 5 days of inoculation, was significantly Phybridization revealed that the number of IL-8 and MIP-1βproducing cells was increased in the infected brain.

在研究过程中，采用了病毒接种技术、普通病理学研究方法、流式细胞仪技术、RT-PCR技术、原位杂交技术、免疫组化技术、凋亡细胞末端标记技术，系统研究了不同日龄的SPF雏鸡人工感染AEV-NH937株后的病理变化，T和B淋巴细胞、T淋巴细胞亚群的动态变化规律，淋巴细胞表面某些粘附分子的变化，淋巴细胞和神经元的坏死与凋亡，雏鸡脑组织中产生趋化因子MIP-1β和IL-8细胞的变化规律，脑组织中围官性细胞浸润的细胞类型。

Sodium citrate; calcium carbonate; calcium hydroxide; potassium bicorbonate; magnesium carbonate; ferrous sulphate; Zinc sulphate; copper sulphate; manganese sulphate; potassium iodide; sodium selenite.

柠檬酸钠；碳酸钙；氢氧化钙；碳酸氢钾；碳酸镁；硫酸亚铁；硫酸锌；硫酸铜；硫酸锰；碘化钾；亚硒酸钠。

All this to say that I'm glad filmmaking hasn't become completely ironic. I can enjoy an ironic film as much as the next person, but they usually involve a message of some sort. One that's delivered in a smirkingly clever way that requires little in the way of talent.

所有这些都是说，我很高兴电影制作没有变成完全讽刺艺术，我可以和下一个人一样享受讽刺电影的乐趣，虽然他们经常会被划归到一定级别，技术方面不需要很高的才能。