查询词典 library binding

Radiolabeled pharmacological binding assay showed: 1 BmK AS bound specifically to a single class of non-interacting binding sites on both rat brain and cockroach nerve cord synaptosomes with high affinity and low low capacity. The association of BmK AS with both synaptosomes was quick to reach equilibrium, but the dissociation seemed to be only partial. The specific binding of BmK AS was independent of membrane potential, and couldn't be modified by veratridine. Moreover, the binding of BmK AS could be inhibited by native BmK AS, BmK AS1, BmK IT2 and BmK IT on both synaptosomes, but not or partially inhibited by BmK Ⅰ and suggesting BmK AS type neurotoxins might bind to a distinct receptor site on mammal and insect sodium channels with a similar manner from β-scorpion toxins.

二。蝎毒素多肽配体与哺乳动物和昆虫靶离子通道受体的药理结合特性及其靶结合位点的研究：经放射性标记结合和竞争抑制实验表明：1）BmK AS在大鼠脑和蟑螂神经索突触体上有单一非协同性高亲和力低结合容量结合位点，能快速结合并达到平衡，但仅能部分解离，其结合不依赖于膜电位，也不受藜芦碱调节，天然的BmK AS，BmK AS-1，BmK IT2和BmK IT可抑制〓AS与两种突触体的特异结合，而BmK Ⅰ无或仅有部分抑制效应。

Of the three binding modes, the intercalative binding is thought to be the strongest binding because it is a type of binding caused by the intercalative molecule plane sandwiched between the aromatic heterocyclic and base group pairs of DNA.

本论文的主要目的是在研究鬼臼金属配合物和铁/锰原子簇化合物与DNA相互作用的基础上，采用紫外光谱、荧光光谱、凝胶电泳和粘度测量等多种研究手段研究了其与DNA的相互作用。

Of the three binding modes, the intercalative binding is thought to be the strongest binding because it is a type of binding caused by the intercalative molecule plane sandwiched between the aromatic heterocyclic and base group pairs of DNA.

本论文的主要目的是在研究鬼臼酰肼金属配合物和铁/锰原子簇化合物与DNA相互作用的基础上，采用紫外光谱、荧光光谱、凝胶电泳和粘度测量等多种研究手段研究了其与DNA的相互作用。

An antibody binding to IGF-IR and inhibiting the binding of IGF-I and IGF-II to IGF-IR which is characterized in that said antibody is a is ofIgG1 isotype, b shows a ratio of IC50 values of inhibition of the binding of IGF-I to IGF-IR to the inhibition of binding of IG-II to IGF-IR of 1:3 to 3:1, c inhibits for at least 80% at a concentration of 5 nM IGF-IR phospohrylation in a cellular phosphorylation assay using 3T3 cells providing 400,000 to 600,000 molecules IGF-IR per cell in a medium containing 0.5% heat inactivated fetal calf serum when compared to such an assay without said antibody, and d shows no IGF-IR stimulating activity measured as IGF-IR phophorylation at a concentration of 10 M in a cellular phosphorylation assay using 3T3 providing 400,000 to 600,000 molecules IGF-IR per cell in a medium containing 0,5% heat inactivated fetal calf serum when compared to such an assay without said antibody has improved properties in antitumor therapy.

一种已经提高了抗肿瘤治疗的特性的抗体，所述抗体结合IGF-IR并且抑制IGF-I和IGF-II与IGF-IR结合，其特征在于所述抗体a是IgG1同种型，b显示其对IGF-I与IGF-IR结合的抑制的IC 50 值与其对IGF-II与IGF-IR结合的抑制的IC 50 值的比率为1∶3-3∶1，c当与没有所述抗体的这种测定比较时，其在5nM的浓度上，在包含0.5％的热灭活胎牛血清的培养基中，使用3T3细胞的细胞磷酸化测定中，抑制至少80％的IGF-IR磷酸化，所述3T3细胞提供400，000-600，000分子IGF-IR/细胞，和d当与没有所述抗体的这种测定相比，在包含0.5％的热灭活胎牛血清的培养基中，使用3T3的细胞磷酸化测定中，其在10μM的浓度上没有显示作为IGF-IR磷酸化所测量的IGF-IR刺激活性，所述3T3提供400，000-600，000分子IGF-IR/细胞。

The experiments confirm that Nuclear fast red bindto DNA to form a 1:1 association complex with the binding constant 7.29×10~5 L/mol,and the major binding mode of Nuclear fast red to DNA is electrostatic binding.However, at high iron strength, the major binding mode changes into intercalativebinding. Part four is about investigation on the interaction of purpurine with DNA.

结果显示，核固红与DNA主要以静电作用模式结合，结合位点为1，结合常数为7.29×10~5L／mol；而在高离子强度下，其主要作用模式改变为嵌插作用；第四部研究了红紫素与DNA的相互作用。

The interaction between MT and AA was expressed by infrared spectra, the functional groups of -SH in MT had lone pair electrons, which could formπ-πwith -C=O in AA. The equilibrium binding experiment and Scatchard analysis showed, at least three kinds of binding sites would exist when MT-imprinted polymer formed, the most adsorption capacities were 3.3896mg/g, 0.9362mg/g, 1.6583mg/g respectively; most terminal thiolate sulfurs in MT were polymerized as binding sites in the process of polymerization, which could observed from TEM, binding capability of other thiolate sulfurs was weak since spatial hindrance existed.

采用红外光谱研究了加入金属硫蛋白后功能单体丙烯酸官能团谱峰的变化，结果表明金属硫蛋白分子中的-SH与丙烯酸中的C=O在氯仿溶液中能形成π-π键，由聚合物的吸附特性和Scatchard分析可以得出，金属硫蛋白印迹聚合物至少存在三种结合位点，其高、中、低的最大吸附量分别为3.3896mg/g、0.9362mg/g、1.6583mg/g；通过透射电镜可以观察到，金属硫蛋白在聚合过程中主要以分子两端的巯基作为结合位点，不同位置的巯基受到的空间位阻不同，导致产生了三种不同的结合位点。

Process and method: 1, chat rooms users to edit the page content Textbox control will change as online open-source online text editor, to increase its diversity. 2, GridView control, the data is dynamic binding using C # code, rather than in the control of the data source through direct binding, the original quot;batch delete quot; button in case of direct binding function can be achieved, but not in the dynamic binding set of data in batch delete, which will quot;bulk delete buttons to modify the code. 3, originally a large number of cascading style sheets .

过程与方法：1、聊天室房间页面中用户编辑聊天内容的Textbox控件将更换成为网上开源的在线文本编辑器，以增加其多样性。2、GridView控件中的数据是利用C#代码动态绑定，而非在控件中通过数据源直接绑定，原&批量删除&按钮在直接绑定的情况下可以实现其功能，但无法在动态绑定中实现数据的批量删除，因而将对&批量删除&按钮代码进行修改。3、原本大量的级联样式表

The objective of the last part is to, primarily, check the binding potentiality of the brain tissues with PrPc and then to determine the brain subfractions which have the most abundant binding proteins for the sake of further isolation of binding proteins, and then to identify and characterize these binding candidates.

新发现、新观点、新创造：首先，目前发现的所有朊蛋白的结合分子由于获取方法所限尚未有证据表明其直接结合，而且对结合功能也多为推测，本研究的立意在于从功能着手，分离和鉴定朊蛋白受体，并进一步鉴定其生理功能。

Teaching materials and teaching and Library Journal-length version of live by binding line production, as printed on the following publications in disc binding package production, on my computer linkage cycle without open beyond off, high-quality goods and excellent products are disk-shaped binding package on this computer.

教材、教辅及图书期刊等长版活均由胶订联动线生产，1万印以下的书刊在圆盘胶订包本机上生产，联动线周期排不开干不完的活、精品和创优品等都在圆盘胶订包本机上生产。

The synthesized cDNA is a continuous band less than 6. 0kb shown on x-film through α〓PdCTP which was inserted in the first strain of cDNA. The titer of the Ha-cDNA library was about 1. 5×10〓cfu/ml which could represent all protein-encoding sequences from Hz-AM1 cells; Namely, the cDNA library has representativity. After the successful construction of the Ha-cDNA library, the open-reading frame of vp39 gene of Heliothis armigera was then fused in frame with the Ga14-DNA-binding domain in the pGBT9 plasmid and the fusion protein Ga14-VP39 was expressed in yeast to be used as bait protein.

在成功构建棉铃虫细胞Hz-AM1cDNA文库的基础上，本文接着将棉铃虫核型多角体病毒衣壳蛋白VP39（HaNPV-VP39）基因克隆到酵母双杂交系统(yeasttwo-hybrid system)中的质粒pGBT9上。pGBT9上含有酵母菌转录激活因子GAL4 DNA结合域的编码区（GAL4-DBD），使克隆到质粒pGBT9上的HaNPV-vp39基因与GAL4-DBD基因融合，在酵母菌中表达DBD-vp39融合蛋白，该融合蛋白即为酵母双杂交系统中的诱饵蛋白。

"Second Life is remarkably easy to work with, and is very popular,"

"第二次生命是显着容易的工作，并且很受欢迎，"

For example, we usually assume that materials are homogeneous and isotropic and free of internal defects or flaws.

为了得到适合有限元分析的模型，我们必须经过如图2所示的简化步骤。