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RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

The nation has become less paternalistic, less likely to think the sanctity of life trumps all else

国家家长专断式作法减少，不再将生命的圣洁性看的高于一切

Determine the prevalence of protein energy malnutrition in population less than 5 years of age. Ascertain the prior nutritional status. Determine the prevalence of micronutrient deficiencies in the population less than 5 years of age (for example, scurvy, anemia, pellagra).

营养 --确定在5岁以下的儿童中患PEM的普遍程度--确定先前的灾民营养水平--确定在5岁以下儿童中患微量营养元素缺乏症的普遍程度（例如：坏血病，贫血，糙皮症

Therefore, in such cases, they immediately make adjustments: In accordance with the housing area of error of 3 percent or less to allow the provisions in excess of the area less than 2.7 square meters of houses, the same can apply for 10% of the down payment.

所以，在出现这种情况后，他们马上作出调整：按照房屋面积误差允许在 3%以内的规定，超出规定面积2.7平方米以内的房屋，同样可以申请10%的首付。

Of endogenous variables with the Root-Mean-Square Percent Error between simulated values and actual values less than 5%, and 81. 8% of endogenous variables with RMSPE less than 10%, which implies that the overall goodness of the model is satisfactory.

检验结果表明，动态模拟值与实际观测值的均方根相对误差低于5%的内生变量占全部内生变量的59.1%，而均方根相对误差不超过10%的内生变量占81.8%，说明模型对观测值具有良好的总体拟合性能。

A simulation model was set up. Simulation outcome shows that compensating torque can be rapidly regulated according to demands of dynamic loading.Stable-state error is less than one percent,and dynamic error is much more less than that of traditional PID.In the

建立了仿真实验模型，运行结果表明：补偿转矩能够根据动态加载的要求进行快速调整；稳态运行速度误差小于1%，动态误差远远小于传统PID控制。

In the best condition of headspace and chromatogram, the mensuration of aquatic trace volatile can get a satisfaction result such as chloroform , carbon tetrachloride , trichloroethylene and perchlorethylene, all relativity standard deviation RSD is less than 4%,all the correlation coefficient R is larger than 0.99, and the relative error is less than 3%.

在选择最佳的顶空和色谱条件下，测定水中痕量挥发物三氯甲烷，四氯化碳，三氯乙烯和四氯乙烯可获得令人满意的结果，相对标准偏差RSD均小于4%，相关系数R均大于0.99，相对误差小于3%。

Leaves less than 1 cm wide; perianth less than 7 mm.

叶少于1厘米宽；花被不到7毫米 3

As a result, you might think American homes of the 90s are less than spotless. Witness the fact that sales of household cleaning products have declined in recent years. Besides that, Americans seem to be less persnickety about their housecleaning--especially in areas that nobody sees.

因此 你可能会认为九○年代的美国家庭大概不一尘不染由家用清洁产品的销售量在近几年下降便可见一般此外美国人似乎对于家庭的清洁比较不那么吹毛求疵了--特别对那些没有人看到的地方吸沙发下的灰尘？

About a Year and half after I had entertain'd these Notions, and by long musing, had as it were resolved them all into nothing, for want of an Occasion to put them in Execution, I was surpriz'd one Morning early, with seeing no less than five Canoes all on Shore together on my side the Island; and the People who belong'd to them all landed, and out of my sight: The Number of them broke all my Measures, for seeing so many, and knowing that they always came four or six, or sometimes more in a Boat, I could not tell what to think of it, Or how to take my Measures, to attack Twenty or Thirty Men single handed; so I lay still in my Castle, perplex'd and discomforted: However I put my self into all the same Postures for an Attack that I had formerly provided, and was just ready for Action, if any Thing had presented; having waited a good while, listening to hear if they made any Noise; at length being very impatient, I set my Guns at the Foot of my Ladder, and clamber'd up to the Top of the Hill, by my two Stages as usual; standing so however that my Head did not appear above the Hill, so that they could not perceive me by any Means; here I observ'd by the help of my Perspective Glass, that they were no less than Thirty in Number, that they had a Fire kindled, that they had had Meat dress'd.

我自从有了这些想法之后，平时就经常会想到这件事，可是因为没有机会付诸实施，因此一直都毫无结果。这样大约又过了一年半光景。一天清晨，我忽然发现有五只独木舟在岛这头靠了岸，船上的人都已上了岛，但却不知道他们去哪儿了。他们来的人这么多，把我的计划彻底打破了。因为我知道，一只独木舟一般载五、六个人，有时甚至更多。现在一下子来了这么多船，少说他有二三十人，我一个人单枪匹马，如何能对付他们呢！因此，我只好悄悄躲到城堡里去，坐立不安，一筹莫展。可是，我还是根据过去的计划，进行作战准备，以便一有机会，立即行动。我等了好久，留神听他们的动静，最后，实在耐不住了，就把枪放在梯子脚下，像平时那样，分作两步爬上小山顶。我站在那里，尽量不把头露出来，唯恐被他们看见。我拿起望远镜进行观察，发现他们不下三十人，并且已经生起了火，正在煮肉。至于他们怎样煮的，煮的又究竟是什么肉，我就不得而知了。

If you were not , and OS X just booted normally, have no fear.

如果你没有，和OS X刚刚启动通常，没有恐惧。

I only want to preserve our all .

我只想保存好我们的一切