查询词典 lamin

"Identification and characterization of autoantibodies against the nuclear envelope lamin B receptor from patients with primary biliary cirrhosis".

&识别和鉴定抗体对核被膜核纤层蛋白B受体例原发性胆汁性肝硬化&。

We report the efficient silencing of both cellular lamin AC and HCV RNAs in Huh-7 hepatoma cell lines supporting HCV replication.

我们报道了在支持HCV复制的Huh-7肝细胞瘤细胞层的细胞层粘连蛋白AC和HCV RNAs的有效的沉默。

Over expressive mutant lamin A is toxic to the development of zygotes, Only zygotes with no target gene integration could survive until to be born.

过量表达的突变核纤层蛋白A对小鼠受精卵有致死性，只有无目的基因整合的受精卵才能存活至出生。

The result showed that lamin B recruited to the chromosome gradually after 6-DMAP was added into the culture solution.

此外，我们采用免疫荧光染色观察PM胚胎核膜重现过程中核纤层蛋白B的动力学变化，结果显示：在加入6-DMAP后核纤层蛋白B在染色体周围逐渐聚集，约3h后核膜完全形成，此时核纤层蛋白B在染色体周围的聚集达到最高峰。

Cells were transfected with lamin A/C siRNA using the transfection reagents indicated, according to the manufacturer's protocol.

RNAiFect 是一种基于脂质的转染试剂，专门用于 siRNA 转染，确保没有 RNAse 活性。

Through PCR and sequencing, a fragement sequence with lamin gene feature was obtained. It was of of transcriptional activity. In addition, we also cloned and identified giardial DNA topoisomerase gene.Our phylogenetic analysis showed that although Giardia diverged early but not first, thus, it is not as primitive as thought before.

然后进行了核纤层蛋白基因的扩增和鉴定，获得了一个具有明显核纤层蛋白基因特征的基因序列片段，并证明它是表达的且不具内含子；此外还克隆鉴定了作为核骨架重要成分的DNA拓扑异构酶II的基因；在此基础上结合已报导的不同进化地位生物上的数据进行了分子系统学分析，结果显示贾第虫虽然分支较早，但不象过去所认为的那么原始。

In the other hard, it is reported that caspase - 3 has important effect in cell apotosis by destroyiny the cell anti - apoptosis factors ( eg: bcl - 2 ), cell framework (eg: frame protein actin, fodrin, lamin).

此外文献报道，CasPase一3还通过破坏细胞抗凋亡因素(如bel一2)，破坏细胞的结构(如结构蛋白aetin、fodrin、larnin)在细胞凋亡中发挥作用。

Results: Twenty one differential protein spots were screened and 5 differential proteins were identified. They included Ezrin protein, transcription elongation factor A, and lamin A/C which were up-regulated in LoVo cells and non-metastatic protein expressed in isoform a (NM23 A) and heat shock protein 8 (HSPA8) which were up-regulated in SW480 cells.

结果：筛选出21个差异蛋白质点，成功鉴定出5种差异蛋白质，其中在LoVo细胞株中表达上调的蛋白有埃兹蛋白、转录延长因子A (transcription elongation factor A)和甘氨酸A/C，在SW480细胞株中表达上调的蛋白有NM23蛋白[non-metastatic cell 1 protein (NM23A) expressed in isoform a]和（heat shockprotein 8，热休克蛋白8）。

Nineteen spots were changed significantly after FA treatment.Thirteen proteins were identified by peptide mass fingerprinting or peptide sequence analysis,including putative nucleoside diphosphate kinase,elongation factor 1-gamma,triosephosphate isomerase,60S acidic ribosomal protein P0,heat shock protein 75 kDa,similar to heat shock 70kD protein binding protein,annexin I,hypothetical protein FLJ34423,microtubule-actin crosslinking factor 1,lamin B2,ATP synthase alpha chain,mitochondrial precursor,proteasome subunit alpha type 6.These identified proteins involved in energy metabolism,translation and RNA processing,protein folding,redox regulation,cell structure and cell signaling.

双向凝胶电泳结果显示，甲醛刺激后19个蛋白斑点发生变化，肽指纹图谱及肽序列标签鉴定了其中13个蛋白斑点，已鉴定的蛋白包括二磷酸核苷酸激酶、延长因子1-γ，磷酸丙糖异构酶、60S酸性核糖体蛋白P0、75kDa热休克蛋白、70kD热休克蛋白样结合蛋白、钙依靠磷脂结合蛋白I、假想蛋白FLJ34423、微管-肌动蛋白交叉连接因子1、核纤层蛋白B2、ATP合成酶α链、蛋白酶体α亚基6，这些蛋白功能涉及转录调节、蛋白折叠、信号传导、能量代谢、细胞骨架等各个方面。

Thirteen proteins were identified by peptide mass fingerprinting or peptide sequence analysis,including putative nucleoside diphosphate kinase,elongation factor 1-gamma,triosephosphate isomerase,60S acidic ribosomal protein P0,heat shock protein 75 kDa,similar to heat shock 70kD protein binding protein,annexin I,hypothetical protein FLJ34423,microtubule-actin crosslinking factor 1,lamin B2,ATP synthase alpha chain,mitochondrial precursor,proteasome subunit alpha type 6.These identified proteins involved in energy metabolism,translation and RNA processing,protein folding,redox regulation,cell structure and cell signaling.

双向凝胶电泳结果显示，甲醛刺激后19个蛋白斑点发生变化，肽指纹图谱及肽序列标签鉴定了其中13个蛋白斑点，已鉴定的蛋白包括二磷酸核苷酸激酶、延长因子1-γ，磷酸丙糖异构酶、60S酸性核糖体蛋白P0、75kDa热休克蛋白、70kD热休克蛋白样结合蛋白、钙依赖磷脂结合蛋白I、假想蛋白FLJ34423、微管-肌动蛋白交叉连接因子1、核纤层蛋白B2、ATP合成酶α链、蛋白酶体α亚基6，这些蛋白功能涉及转录调节、蛋白折叠、信号传导、能量代谢、细胞骨架等各个方面。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。