查询词典 labelled graph

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

The 33P, 32P double label tracing technique was carried out to study the effective fertilization of phosphorus for citrus plantation in mountain area of northern Taiwan. 33P labelled orthophosphoric acid was applied in a standard position with respect to the tree and 32P labelled orthophosphoric acid at a location and rate corresponding with the treatments The results showed that the variability o1 the 33P/32P ratios (49%) was much lower than that of single 32P activity counts (72%).

应用磷－33，磷－32重复标识法，研究本省北部山区柑园有效施肥问题；其法将磷－33标识正磷酸，依标准法施用於根羣密叶区域，又以磷－32标识正磷酸以不同施用量，处理於不同深度，结果显示以33P/32P比率所得试全变异(49%)达较单独测算32P放射活性者(72%)为小。

The weakly or strongly stained CaBP-LI/CB-Audouble-labelled cells gradually increased caudalward.In the middle rostrocaudal third of the red nucleus,the double-labelled neurons mainly occupied the ventromedial portions of the red nucleus.

两种神经元在红核具有特殊的分布方式，头侧水平CB-Au标认的红核-脊髓投射神经元均呈CaBP-LI阴性，向尾侧CaBP-LI阳性神经元数目逐渐增多。

The weakly or strongly stained CaBP-LI/CB-Audouble-labelled cells gradually increased caudalward.In the middle rostrocaudal third of the red nucleus,the double-labelled neurons mainly occupied the ventromedial portions of the red nucleus.

两种成年人神经元在红核具有特殊的分布方式，头侧水平CB-Au标认的红核-脊髓投射成年人神经元均呈CaBP-LI阴性，向尾侧CaBP-LI阳性成年人神经元数目逐渐增多。

The results showed that ①after the treatment with 0. 3% Triton X-100 5 min or standard binding buffer 20 min,the proteins on NCM could be detected with first specific antiserum from the titer 1:100 diluted to 1: 1000;②the blocking effect of Tween 20 was more effective than that of other blockings;③both HRP-labelled conjugate and colloid gold-labelled c...

结果表明：①经（TritonX－100）＝0.3％，5min和SBB20min的蛋白质复性处理后，可使第一免疫血清稀释度1：100改为1：1000使用；②应用（Tween20）＝0.05％作为封闭液效果最好；③金标法及酶标法都可用于Western印迹技术，酶标法主带清晰，而金标法灵敏度高。

Of the above-mentioned immune molecules, microglia labelled with OX-42 or ED-1 were increased in their populations and staining intensity in LTA-treated rats than those in saline-treated ones. It is also true for OX-42 or ED-1 labelled macrophages in the meninges and the choroids plexuses.

结果显示，受OX-42和ED-1标志之小神经胶细胞在胼胝体处的细胞数量，与出生后七天对照组比较，其细胞数量有明显的增加，免疫染色深度也较深；脑膜及脉络丛内的巨噬胞也有类似的改变。

To resolve the algorithm of the chromatic number of a graph,a special method is adopt to remember the coloring process of graph,and the method does different jobs for four given cases during the coloring process.The adjacent strong edge chromatic number of join graph of star and path is obtained.

为了解决图的邻强边染色问题中一个图的色数算法问题，通过特别的方法来记图的染色过程，同时分4种情况讨论了星和路联图的邻强边染色问题，指出在染色过程中给定的4种情况的染色方法各不相同，并通过对图的着色得到了星和路联图的邻强边色数。

In fact, researching the symmetry property of the Coset graph is more significant than the Cayleygraph, because every vertex-transitive graph is always some Coset graph of its full automorphism group.

关于陪集图对称性的研究其实较Cayley图更具有普遍的意义，因为任何一个点传递图都是其全自同构群的某个陪集图。

Further, we can also define if a Coset graph is a graph representation, we note it GR for short, that is, the relevant group is exactly equal to the full automorphism group of the Coset graph.

甚至，我们也可以定义一个陪集图是一个图表示，简称GR，即相应的群是否恰等于它的这个陪集图的全自同构群。

In fact, researching the symmetry of Sabidussi coset graphs has more general significance than doing so for the Cayley graph because every vertex-transitive graph is always some coset graph of its full automorphism group.

关于陪集图对称性的研究其实较Cayley图更具有普遍的意义，因为任何一个点传递图都是其全自同构群的某个陪集图。

A historical region of southwest France between the Pyrenees and the Garonne River. The duchy of Aquitaine was joined with France after the marriage of Eleanor of Aquitaine to King Louis VII in1137, but its possession was disputed after her subsequent marriage to Henry II of England.

阿基坦法国西南部一个历史地区，位于比利牛斯山和加隆河之间，在1137年阿基坦的埃莉诺与国王路易斯七世结婚之后阿基坦公国加入法国，但当她再嫁英国亨利二世后，其归属权便受到争议

There are moments when I look at the smiling , happy faces of my expectant staff and wonder how they will cope when confronted by an outraged housemaster along with an unrepentant and recalcitrant bleached blond teenager in 18 years ' time .

有时候，我看着那些满怀期待的员工微笑，幸福的脸，想着当他们遇到一位愤怒的宿舍管理员和一位不知悔改，叛逆，而且将头发染成白色的18岁少年时，他们会如何处理。