查询词典 kit

A kit for vaginal self-use by a female patient in protection against contracting viral infection and against conceptions as a result of normal heterosexual intercourse, said kit comprising a vaginal applicator comprising a vaginally insertable tubular member having a length of about five inches, and having an aperturable distal end adapted to be positioned adjacent to the patient's cervix when the applicator is fully inserted into the patient6's vagina, and at least one unit dose of the spermicidally and virucidally effective formulation of claim 1, said unit dose being form about 5-10 mls thereof.

一套女性患者阴道自用的工具，以防止感染病毒或因异性间正常性行为而受孕。该套工具包含一个阴道给药器（由一个长约5英寸、末端有孔眼、可插入阴道的管状构件构成，当给药器完全插入患者阴道后，构件的末端适合置于患者的宫颈附近），以及至少1个单位剂量（5-10毫升）的、能有效杀死精子和病菌的权利要求1中的配方。

For the leatherworkers, Aldor will offer a Vindicator's Armor Kit, which adds 8 defense ratings on chest, hands, legs, or feet, while Scryer will offer a Magister's Armor Kit that adds 3 mana every 5 seconds to chest, hands, legs, or feet.

对于皮革工人，阿尔多尔将提供的维护者的装甲组件，它增加了8胸部防御率，手，腿，脚，而占星将提供一个硕士的装甲工具包，增加了3法力每5秒的胸部，手，腿，或脚。

The microspheres were conjugated with FITC antibody, and the reagent kit HCG SEROZYME was used to test the capacity of conjugation. The results indicated that the conjugation capacity of the microspheres was similar with that of the Italy microspheres, which were merchandises for the reagent kit HCG SEROZYME specially.

另外，还将微球与荧光标记的FI-TC蛋白进行偶联，用试剂盒HCG SEROZYME检测了偶联的情况，并将结果和与试剂盒配套的意大利磁珠所得到的结果进行了比较，发现两者偶联效果基本接近。

The Mickeys Toon Town kit is packed into a bright yellow school tote, while the Stitch kit is contained inside an attractively-designed messenger bag featuring the playful Disney character on the front.

Mickeys Toon Town」以精美的黄色小书包作包装设计，而「史迪仔」精品则以附有该卡通人物的单肩背包盛载，对小朋友深具吸引力。

A competitive ELISA kit for detect ENR was developed with ENR mAb and its traits were tested.Two hybridoma lines were filtered and the best one was 4G1-B3,which secreted ENR mAb with indirect ELISA titers of 1∶1.024×10~6 in ascites.Isotype of the two mAb was IgG_1.ENR mAb had a high affinity constant with 9×10~(10)L/mol.The ENR-Kit had the linear detection range of 0.05～101.6μg/L,the sensitivity of 0.05 μg/L and a good sensitivity with an IC_(50) of 1.1μg/L to ENR,cross-reactivity to other compounds less than 0.01%.The average recoveres of ENR spiked in chicken,liver,fish and milk were 81.5%,87.6%,84.3% and 95% respectively.The coefficient variation was below 10%.

结果表明：筛选出的2株杂交瘤细胞，单抗亚类均为IgG1型，其中4G1-B3株的ENR mAb间接ELISA效价为1：1.024×106，亲和常数为9×1010L/mol；竞争ELISA试剂盒的线性检测范围为0.05~101.6μg/L、灵敏度0.05μg/L、半数抑制浓度(IC50)1.1μg/L，与其他化合物的交叉反应率均小于0.01%，鸡肉样、鸡肝样、鱼肉样和牛奶样的平均添加回收率分别为81.5%8、7.6%、84.3%和95%，不同基质添加恩诺沙星标准品做竞争ELISA对ENR-Kit检测结果影响小，试剂盒保存期6个月以上。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Objective To evaluate Borna disease virusFluorescent-Quantitation Real Time PCR kit and study the preliminary application results of the kit.

目的 评价博尔纳病病毒(Borna disease virus， BDV)实时荧光定量PCR试剂盒的各项指标，并了解其实际应用效果。

Important things that should always be a part of your repair kit are spoke tool, spanners, screwdriver, pump, Allen key, puncture repair kit, a bottom bracket tool as well as some lubricant.

重要的事情，应该永远是部分的修理工具的发言工具，扳手，螺丝刀，泵，阿伦关键，穿刺修理包，一中轴工具，以及一些润滑剂。

Must have items for short riding trips include wrenches for all the nuts and bolts on the bike, tire levers, pump, patch kit/tubes, chain tool, crescent wrench, zip ties, duct tape and a small first aid kit.

项目必须有短扳手骑马旅行包括所有的具体细节在自行车，轮胎杠杆，泵，补丁包/管，链条工具，月牙扳手，拉鍊关系，胶带和一个小急救箱。

Kit took advantage of the opportunity to spin her name and used Kit Kat wrappers to make a corset with oversized chocolate-colored lacing in the back.

Kit利用这次的机会纺自己的名子。他用Kit Kat包装纸做一件背后超大巧克力色饰带的马甲。

Fostering the concept of multi-culture firmly and mastering the development of advanced culture correctly are conductive to resisting terrorism, separatism of nationalities and ultraism of religion, which is of great academic theoretical, immediate and profound historical si...

牢固树立多元文化的理念和正确把握先进文化的发展方向，有助于在世界范围内自觉地抵制恐怖主义，民族分裂主义和宗教极端主义。不仅具有重大的学术理论意义，而且也具有重大的现实意义和深远的历史意义。

But if anyone walks in the night, he stumbles, because the light is not in him.

11：10 若在黑夜行走，就必碰跌，因为那光不在他里面。