查询词典 kit

The rate of c-kit+ cells in the heart increased gradually from E10 with the highest level of 12.6±3.2% at E11, which was nearly similar to the fetal liver, except for E12 when the c-kit+ cells was 3.4±1.2% in the heart compared to the fetal liver with 11.6±4.1% c-kit+ cells. The rate of c-kit cells in the suspension cells from the heart at E11 maintained stable after 24h culture based in the stromal cells of the heart and AGM region with the same conceptus age, but the rate of c-kit cells obviously decreased in the condition of the stromal cells from the fetal liver. The c-kit cells of the three conditions were all at low level at 48h without significant difference.

通过流式检测c-kit+细胞我们发现，胚胎期心脏和胎肝c-kit+细胞比率几乎一致，E11时c-kit+细胞明显增多，分别高达(12.6±3.2)%和(9.6±2.8)%，但E12时心脏中c-kit+细胞明显减少，仅为(3.4±1.2)%，此时胎肝中c-kit+细胞为(11.6±4.1)%。E11的悬浮细胞与心脏内皮细胞和AGM区基质共培养24h时，c-kit+细胞数量保持稳定，但在胎肝基质细胞中c-kit+细胞数量明显减少，在共培养48h，三组中的c-kit+细胞均呈较低水平，组间没有显著性差异。

The calibration curve of the SM2-Kit with standard SM2 inhibitor was typical sigmoid curve fitted to the four parameter logistic equation with the linear detection of 0.625 μg/L to 80.0 μg/L (R2=0.9911), the sensitivity of 0.9 μg/L, the IC50 of 5.82 μg/L and the detection limit of 1 μg/L. The recoveries of SM2 spiked in pig feed were 83.4 %, in pig urine were 89.5 %. The precision and accuracy of the assay as determined by inter-assay and intra-assay coefficient variation was both below 15 %. The SM2-Kit generally had 2.08 % cross-reactivity towards sulfamerazine and little or no cross-reactivity towards other sulfonamides. The dilution solution of SM2 had no effect on results of SM2-Kit. The validity of SM2-Kit in 4 ℃ was above six months.

研究结果表明，SM2-Kit的标准曲线呈典型的S型，相关系数R2=0.9911，符合4参数logit曲线拟合，线性检测范围为0.625 μg/L～80 μg/L，灵敏度为0.9 μg/L，半数抑制浓度(IC50)为5.82 μg/L，检测限为1 μg/L；饲料样、猪尿样的平均添加回收率分别为83.4 %、89.5 %，平均批内和批间变异系数均低于15 %；SM2-Kit与磺胺甲基嘧啶的交叉反应率为2.08 %，与其它磺胺类药几乎没有反应性；基质对SM2-Kit的检测结果影响不大；试剂盒在4 ℃可保存6个月。

Than between the rN-ELISA and IDEXX ELISA kit (90.8%), therefore the rNM-ELISA was selected as a optimal method to prepare kit for determine antibodies to PRRSV. The storage life of the rNM-ELISA kit at 4℃ and -20℃ was 12 months at least by adding protein stabilizer to reagents in the kit, this provided pig industry for our country with a serodiagnostic method which had advantages of high sensibility、strong specificity、long period of validity、safety and no shedding PRRSV.

将建立的rN-ELISA和rNM-ELISA分别与IDEXX ELISA试剂盒进行比较检测327份猪血清，结果rN-ELISA和rNM-ELISA的特异性差异不大，rNM-ELISA与IDEXX ELISA试剂盒的阳性符合率相对较高（92.3％），选择rNM-ELISA作为制备PRRSV抗体检测试剂盒的方法。

Experimental agents: 5% lidocaine hydrochloride;20% ethyl carbamate; aether;pure oxgen and nitrogen for medical purpose; 4% paraform;0.1M PBS; TTC;Bcl-2 andBax immunohistochemistry; SABC kit;~(99m)TcO_4~-, Annenxin V, Sn~(2+)-Tricine Kit andHYNIC-Annexin V Kit.

TTC，2.5％戊二醛，1％锇酸，Epon812；兔抗兔Bcl-2和Bax多克隆抗体、SABC试剂盒、TUNEL试剂盒和DAB染色液；~(99m)TcO_4~-，Annenxin V，Sn~(2+)-Tricine Kit和HYNIC-Annexin V Kit。

In order to verify the Fluorescent-Quantitation PCR kit specific for Borna disease virus nucleoprotein, compare molecular beacon with common probe and study its practical application, the sensitivity, distinctness, repeatability and stability were tested on BDV and non-BDV infected cells, as well as non-infected controls. Human and animal samples were also tested by the kit. The lowest quantity of virus RNA detected by the kit was 2.5×101, which corresponded to 1 virus copy.

为评价博尔纳病病毒(Borna disease virus， BDV)核蛋白荧光定量PCR试剂盒的各项指标，比较分子信标探针相对普通探针的优势，并了解其实际检测效果，本课题组使用BDV OL持续感染细胞株、非BDV病毒序列转染的OL细胞、正常的OL细胞，对BDV RT-PCR试剂盒的敏感性、特异性、重复性和稳定性进行评估，同时检测部分临床病人和动物外周血液RNA。

Has: Blow-box tool kit sets; injection tool box sets, bubble shell tool sets, metal box tool kit tool kit.

有：吹塑盒工具套组套；注塑盒工具组套，泡壳工具组套，金属盒工具组套，工具包，。

The Promega commercial DNA extraction kit was used as control. Results The Promega kit resulted in a higher copy number of COWP gene[（6.45-9.86）×106]than that of Generay commercial DNA kit [（2.38-3.69）×106 ], 5% guanidine thiocyanate[（1.27-21.29）×105] or 2% Triton X-100 [（2.06-866.70）×103], respectively.

结果 Promega试剂盒的裂解液裂解隐孢子虫卵囊的效果最好，纯化所得卵囊COWP基因拷贝数可达（6.45～9.86）×106；其他依次为捷瑞试剂盒的裂解液[（2.38～3.69）×106]、5%异硫氰酸胍[（1.27～21.29）×105]和2% Triton X-100[（2.06～866.70）×103]。

We pay great attention to technical development, strengthen project quality control, and have successfully developed a large number of new techniques such as hydraulic plate lifting, big formwork construction, fast slip-form construction, cast on site plate technical kit, high-rise building steel structure construction technical kit, high-rise building structural engineering construction technical kit, and earthquake-resistant strengthening technique.

公司注重发展科技进步，强化工程质量管理，先后研制成功液压板技术、大模板施工技术、快速滑模施工技术、现浇平板成套施工技术、高层钢结构成套施工技术、高层结构工程成套施工技术、抗震加固施工技术等一大批科研成果。

Help prepare the documentation for verification and validation of FABP diagnostic kit and ALB diagnostic kit.

协助准备验证和确认FABP诊断试剂和ALB诊断试剂所需文档。

Parliamentary Opposition Leader Lim Kit Siang has on last Thursday submitted a written motion to the Speaker under Standing Order 27(3) to refer MP for Kuala Kangsar and Minister for International Trade and Industry, YB Rafidah Aziz to the Committee of Privileges to inquire whether there is any breach of privilege in her written answer to YB Parliamentary Opposition Leader and MP for Ipoh Timor Lim Kit Siang on 20th September 2005 when answering the question on "the abuses in the issue of APs to import cars, why the sudden jump in the issue of APs in 2004 and 2005 as compared to previous years, and release the full APs lists since 1987 including individual APs."

国会反对党领袖林吉祥已经在上星期四在议会常规第27（3）条文下提呈一项动议，以便把将江沙区国会议员兼国际贸工部部长拿督斯理拉菲达交由国会特权委员会，要求该委员会调查到底贸工部部长在一份于2005年9月20日的一项回答国会反对党领袖林吉祥的书面回答（即一项有关AP分发、2004年和2005 年的AP数目远远超过过往数年所分发的AP数量以及自1987年以来的个人AP持有者的名单）中存有任何违反国会特权的因素。

Fostering the concept of multi-culture firmly and mastering the development of advanced culture correctly are conductive to resisting terrorism, separatism of nationalities and ultraism of religion, which is of great academic theoretical, immediate and profound historical si...

牢固树立多元文化的理念和正确把握先进文化的发展方向，有助于在世界范围内自觉地抵制恐怖主义，民族分裂主义和宗教极端主义。不仅具有重大的学术理论意义，而且也具有重大的现实意义和深远的历史意义。

But if anyone walks in the night, he stumbles, because the light is not in him.

11：10 若在黑夜行走，就必碰跌，因为那光不在他里面。