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The rate of c-kit+ cells in the heart increased gradually from E10 with the highest level of 12.6±3.2% at E11, which was nearly similar to the fetal liver, except for E12 when the c-kit+ cells was 3.4±1.2% in the heart compared to the fetal liver with 11.6±4.1% c-kit+ cells. The rate of c-kit cells in the suspension cells from the heart at E11 maintained stable after 24h culture based in the stromal cells of the heart and AGM region with the same conceptus age, but the rate of c-kit cells obviously decreased in the condition of the stromal cells from the fetal liver. The c-kit cells of the three conditions were all at low level at 48h without significant difference.

通过流式检测c-kit+细胞我们发现，胚胎期心脏和胎肝c-kit+细胞比率几乎一致，E11时c-kit+细胞明显增多，分别高达(12.6±3.2)%和(9.6±2.8)%，但E12时心脏中c-kit+细胞明显减少，仅为(3.4±1.2)%，此时胎肝中c-kit+细胞为(11.6±4.1)%。E11的悬浮细胞与心脏内皮细胞和AGM区基质共培养24h时，c-kit+细胞数量保持稳定，但在胎肝基质细胞中c-kit+细胞数量明显减少，在共培养48h，三组中的c-kit+细胞均呈较低水平，组间没有显著性差异。

The spermatogonium puirity (including the PGP9.5 cells and the c-kit cells) to achieved above 82% by percoll density of 27-35% and panning method and PGP9.5 cells were (63.6±3.2)% and (71.9±4.1)% respectively.

通过收集27~35%Percoll梯度间的细胞或盘化法纯化后，回收精原细胞纯度(包括PGP9.5 和c-kit 细胞)均达到82%以上，其中PGP9.5 细胞分别占(63.6±3.2)%和(71.9±4.1)%，而c-kit 分别占(21.2±1.8)%和(11.5±0.8)%。

The calibration curve of the SM2-Kit with standard SM2 inhibitor was typical sigmoid curve fitted to the four parameter logistic equation with the linear detection of 0.625 μg/L to 80.0 μg/L (R2=0.9911), the sensitivity of 0.9 μg/L, the IC50 of 5.82 μg/L and the detection limit of 1 μg/L. The recoveries of SM2 spiked in pig feed were 83.4 %, in pig urine were 89.5 %. The precision and accuracy of the assay as determined by inter-assay and intra-assay coefficient variation was both below 15 %. The SM2-Kit generally had 2.08 % cross-reactivity towards sulfamerazine and little or no cross-reactivity towards other sulfonamides. The dilution solution of SM2 had no effect on results of SM2-Kit. The validity of SM2-Kit in 4 ℃ was above six months.

研究结果表明，SM2-Kit的标准曲线呈典型的S型，相关系数R2=0.9911，符合4参数logit曲线拟合，线性检测范围为0.625 μg/L～80 μg/L，灵敏度为0.9 μg/L，半数抑制浓度(IC50)为5.82 μg/L，检测限为1 μg/L；饲料样、猪尿样的平均添加回收率分别为83.4 %、89.5 %，平均批内和批间变异系数均低于15 %；SM2-Kit与磺胺甲基嘧啶的交叉反应率为2.08 %，与其它磺胺类药几乎没有反应性；基质对SM2-Kit的检测结果影响不大；试剂盒在4 ℃可保存6个月。

Dimeric structure and several signaling pathways have thus far been proved to be closely correlated with the activation of c-Kit.

迄今为止，二聚化结构及磷酯酰肌醇3′-激酶（PI3K）、JAK-STAT等十几条激酶介导的信号通路被陆续证实与c-Kit的活化密切相关，而针对抑制c-Kit活化设计的靶向药物也陆续投入应用，与特异性的单克隆抗体一起在临床相关肿瘤的诊断及治疗等方面起到了关键性的作用。

Objective To investigate the expression of c-kit in human intermediate-spl it-thickness skin grafts and normal skin, and to recognize the role of c-kit in hyperpigmented process of the skin grafts.

目的 检测c-kit 在人自体移植中厚皮片中与正常皮肤中的表达差异，初步探讨c-kit 在自体移植皮片色素沉着发生机制中的作用。

Experimental agents: 5% lidocaine hydrochloride;20% ethyl carbamate; aether;pure oxgen and nitrogen for medical purpose; 4% paraform;0.1M PBS; TTC;Bcl-2 andBax immunohistochemistry; SABC kit;~(99m)TcO_4~-, Annenxin V, Sn~(2+)-Tricine Kit andHYNIC-Annexin V Kit.

TTC，2.5％戊二醛，1％锇酸，Epon812；兔抗兔Bcl-2和Bax多克隆抗体、SABC试剂盒、TUNEL试剂盒和DAB染色液；~(99m)TcO_4~-，Annenxin V，Sn~(2+)-Tricine Kit和HYNIC-Annexin V Kit。

A competitive ELISA kit for detect ENR was developed with ENR mAb and its traits were tested.Two hybridoma lines were filtered and the best one was 4G1-B3,which secreted ENR mAb with indirect ELISA titers of 1∶1.024×10~6 in ascites.Isotype of the two mAb was IgG_1.ENR mAb had a high affinity constant with 9×10~(10)L/mol.The ENR-Kit had the linear detection range of 0.05～101.6μg/L,the sensitivity of 0.05 μg/L and a good sensitivity with an IC_(50) of 1.1μg/L to ENR,cross-reactivity to other compounds less than 0.01%.The average recoveres of ENR spiked in chicken,liver,fish and milk were 81.5%,87.6%,84.3% and 95% respectively.The coefficient variation was below 10%.

结果表明：筛选出的2株杂交瘤细胞，单抗亚类均为IgG1型，其中4G1-B3株的ENR mAb间接ELISA效价为1：1.024×106，亲和常数为9×1010L/mol；竞争ELISA试剂盒的线性检测范围为0.05~101.6μg/L、灵敏度0.05μg/L、半数抑制浓度(IC50)1.1μg/L，与其他化合物的交叉反应率均小于0.01%，鸡肉样、鸡肝样、鱼肉样和牛奶样的平均添加回收率分别为81.5%8、7.6%、84.3%和95%，不同基质添加恩诺沙星标准品做竞争ELISA对ENR-Kit检测结果影响小，试剂盒保存期6个月以上。

Kit took advantage of the opportunity to spin her name and used Kit Kat wrappers to make a corset with oversized chocolate-colored lacing in the back.

Kit利用这次的机会纺自己的名子。他用Kit Kat包装纸做一件背后超大巧克力色饰带的马甲。

Produced by Miten and Kit Walker (Walela, Kitaro, Pagan Love Orchestra), Embrace features a rich, elegantly developed musical dynamic, due in large part to virtuoso performances by such top flight musicians as Jai Uttal, Kit Walker (keyboards, grand piano, Hammond B3), Nepali native Manose, Miten, Ty Burhoe (a star protégé of Zakir Hussain) and Marquinho Brasil (assorted exotic hand percussion).

由Miten、Kit Walker等人共同制作出来的，Embrace这一张专辑的特色，就是整张音乐的扩张性很大，让人感觉到源源不绝的活力，专辑中有很大的部分是由顶尖的音乐演奏家演奏的，像是Jai Uttal、Kit Walker（键盘手，平台式钢琴，汉孟牌电子琴）、在尼泊尔土生土长的Manose、Miten、Marquinho Brasil（各式各样、含各国情调的打击乐器）。

The density of c-kit+ICCs in submucosa, circular layers and around the myenteric plexuses in HD distal colonic wall was reduced compared to that of HAD and more reduced compared to that of normal control.

实验结果：我们观察到，正常对照组近端和远端结肠壁粘膜下层、肠肌层内弥散分布着中等数量的c-kit+ICCs，肌间神经丛周围分布有大量的c-kit+ICCs，并相互连接成网络状结构。

But in the course of internationalization, they meet with misunderstanding and puzzlement.

许多企业已经意识到了这一点，但在国际化的进程中，仍存在一些误区与困惑。

Inorder toaccomplish this goal as quickly as possible, we'll beteamingup with anexperienced group of modelers, skinners, and animatorswhosenames willbe announced in the coming weeks.

为了尽快实现这个目标，我们在未来数周内将公布与一些有经验的模型、皮肤、动画制作小组合作。