查询词典 isolate

The protocol for the detections of all the AIV isolates, the H5-subtype and the optimization of reactions were studied with a H5-subtype reference isolate BSG1. A 229bp-band of AIV specific and a 380bp-band of H5 subtype specific were amplified individually with the designed primers of AIV specific and H5-subtype specific respectively by the developed RT-PCR technique from isolate BSG1. A double-PCR was also developed which can detect all the AIVs and identify the H5-subtype AIVs. The results of specificity experiment showed that there was no specific band could be amplified with the samples of Newcastle disease virus, infectious bronchitis virus, infectious laryngotracheitis virus, egg drop syndrome virus and infectious bursal disease virus.

结果应用通用引物可从BSG1毒株扩增到与预计大小相符的229bp AIV特异性片段，应用H5亚型鉴定引物则可从BSG1毒株扩增到与预计大小相符的380bp H5亚型特异性片段；在此基础上建立了可检测所有AIV毒株以及可同时进行H5亚型毒株鉴定的二重RT-PCR方法；检测方法的特异性试验结果表明，该方法对新城疫病毒、传染性支气管炎病毒、传染性喉气管炎病毒、产蛋下降综合症、传染性法氏囊病毒无交叉反应；检测方法的敏感性试验结果表明，通用引物扩增、H5亚型特异性引物扩增以及二重PCR扩增反应的组织样品总RNA的最低检出量分别为0.0217μg、0.0217μg和2.17μg；对20份临床样品检测中，有10份为AIV阳性，其中有5份为H5亚型病毒阳性。

And PG activities of pathogenic isolate was obviously higher than that of nonpathogenic isolate, It is same that PG activities of all isolates were the highest in the seventh day after cultivating.

综上所述，PG活性的高低与大豆疫霉根腐病菌的致病力强弱有一定的关系，是该病菌的一种起致病作用的细胞壁降解酶。

To demonstrate experimentally the generation of velogenic NDV from a nonpathogenic waterfowl isolate, we passaged an avirulent goose isolate in chickens.

为了通过实验证明从水禽中分离的非致病型NDV可以转变为速发型病毒，我们通过在鸡体内传播鹅源性无毒力株，经气囊接种连续传代 9次，随后再在鸡脑内传代 5次。

Grisea have been obtained in recent years as well.In our previous work, an RIL population consisting of 304 lines was constructed from the indica cross Zhong 156/Gumei 2. Two genes conferring complete resistance to blast isolate 92-183 (ZC15) were primarily mapped by using 148 lines of the population. In the present study, genotype of the 304 RILs were analyzed and QTL mapping for partial resistance to 2 isolates of leaf blast and 1 isolate of neck blast were conducted.

本实验室曾经应用中156／谷梅2号构建了由304个重组自交系(recombinant inbred lines，RIL)组成的重组自交系群体，应用其中的148个株系对抗稻瘟病基因进行了初步的定位，本研究则进一步对整个群体的304个RILs的基因型进行了分析，对控制叶瘟和穗瘟部分抗性的QTL进行了定位。

While nine Korean isolates were similar to the Japanese isolate red sea bream iridovirus, one isolate was distinct from other iridovirus isolates.

而韩国真鲷虹彩病毒的10个株系和日本株系比较相似，其中一个病毒株系和其他株系明显有别。

Results: The homololgy of nuclcotide sequence between Wuhan isolates and Bejing isolate were semblable, had some difference between Japan isolate and much different with TT virus.

结果：武汉SEN病毒株与北京株同源性较高，与日本株有一定的差异，和TT病毒有较大差异。

To isolate He always feels isolate being a new settler of this country which is completly strange to him.

2做母亲的有时候不能察觉他们所深爱的孩子的过错，这样做的结果使孩子们再次犯错。

The two kinds of clefts are: unoperated isolate cleft palate and unilateral cleft lip and palate with lip only operated. Considering the effect of age on the results, The samples were divided into two age groups: mixed dentition stage and adult permanent dentition stage. The cases of these two groups of unoperated isolate cleft palate patients are 16 and 25 respectively, and those of unilateral cleft lip and palate with lip only operated are 15 all.

考虑到青春期生长发育对研究结果的影响，又分别将这两种类型的患者按年龄分成混合牙列期和成人恒牙列期两个年龄段，与相应年龄段的单纯腭裂术后患者、单侧完全性唇腭裂术后患者及非唇腭裂正常者进行比较，单纯腭裂未手术的患者混合牙列期和成人恒牙列期例数分别为16例和25例，单侧完全性唇腭裂仅修复唇裂患者混合牙列期和成人恒牙列期例数均为15例。

Result The zoospore progenies of isolate HN8-Mt and isolate HN3-Mt had the similar characteristics such as growth rate, colony morphology and the resistance to metalaxyl with that of their parents.

结果］辣椒疫霉Mt突变株的生长速率、菌落形态以及对甲霜灵的抗性等性状在其游动孢子后代中未发生变异。

Firstly, the SSH was used to isolate tissues or organs specific expression genes in different development stages of plant. These genes provide evidence for explaining the molecular mechanism of plant development. Then SSH was widely used to isolate genes related to resistance under biological or abiological stress in different plants and the molecular mechanism of the resistance was primarily elucidated.

首先， 官中的组织特异性表达的基因，为揭示植物生长发育过程的分子机理提供了有效手段；另外，在不同植物中，该技术被大量应用于分离各种生物及非生物胁迫条件下诱导表达的抗性相关基因，可以揭示植物抗逆的分子 SSH 技术已开始应用于分离与次生代谢产物合成相关的基因。

Chimborazo and Cotopaxi, took me by the hand.

越过琴博腊索山和科托帕克西山。

This car is in a good condition.

这辆车的状况很好。