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Be sure to insert the correct name of your friend when you tell them "snaps."

当你叫你的朋友弹指的时候，确保你投入的是你朋友的名字没错。

The high price of wild C. sinensis has led unscrupulous harvesters to insert twigs or even lead wires into the stromata, thereby increasing the weight and price paid. Cultivated C.

野生虫草的高价导致不道德的采集者在基质中灌入细枝或者甚至是铅线，因此增加了重量和价格支付。

Results: This study established a web-based automatic syndromic surveillance system which could utilize the Internet to upload the data files, implement statistical analysis regularly, insert new data into database automatically , offer various types of statistical graphs and GIS (geographical information system) maps, and present the latest epidemic situation materials on the webpage.

研究结果：本研究建置之症候群监测系统，利用网际网路上传资料档案，定时排程执行统计分析，即时更新资料库内容，透过网页呈现最新的疫情资料统计图表，透过多重的查询介面，防疫工作人员能藉此对异常警示作更进一步的分析与确认，降低疫情误判的发生。

Removing the PGE2 vaginal insert will usually help reverse the effects of the hyperstimulation and tachysystole.

PGE2阴道制剂的取出通常有助于逆转子宫过度刺激和收缩过速。

Another pair of primers including the start codern and the terminer coden were designed to amplify the PAL gene. Two restriction enzyme XbaI and XmaI were added to the two primers respectively, which enable to insert this fragment to the prokaryotic expression vetor pCAMBIA2301-WCS.

在PAL 基因的编码框以启始密码子ATG 和终止密码子TAG 为两端设计一对特异引物，分别在引物5' 端引人XbaI 和XmaI 切位点，用来扩增PAL 基因并与pCAMBIA2301-WCS 构建表达载体pCAMBIA2301-PAL。

Another pair of primers including the start codern and the terminer coden were designed to amplify the PAL gene. Two restriction enzyme XbaI and XmaI were added to the two primers respectively, which enable to insert this fragment to the prokaryotic expression vetor pCAMBIA2301-WCS.

在PAL 基因的编码框以启始密码子ATG 和终止密码子TAG 为两端设计一对特异引物，分别在引物5' 端引人XbaI 和XmaI 酶切位点，用来扩增PAL 基因并与pCAMBIA2301-WCS 构建表达载体pCAMBIA2301-PAL。

The efficiency of selection was monitored by comparing the number of phage recovered from the acid elution and cell lysate in each round,and by testing EGFR binding specificity of polyclonal phagescFv on CHOEGFRGFP1 and CHOK1 cell with cell ELISA. Bacterial PCR was used to select clones containing a 1 kb insert. Cell ELISA was used to determine EGFR binding specificity of monoclonal pscFv on EGFR positive and negative cell. The number of individual EGFRbinding clones was determined with nucleotide sequencing. Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection.

以稳定转染的CHOEGFRGFP1细胞和未转染的CHOK1细胞分别作为EGFR阳性和阴性细胞，采用负筛选的方法进行筛选；通过比较每轮投入及洗脱出噬菌体的效价比以及细胞ELISA检测多克隆pscFv与阳性、阴性细胞结合情况对筛选过程进行监测；采用菌落PCR挑选含有全长scFv片断的菌落，进一步用细胞ELISA检测单克隆pscFv与EGFR阳性及阴性细胞结合特异性；挑选EGFR特异性单克隆pscFv采用DNA测序法确定克隆多样性。

Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection. A signal significantly greater than background binding was observed from the third to the fifth round of selection on CHOEGFRGFP1 and CHOK1 cell, but a part of polyclonal scFv bound EGFR specially. About 45% of the selected clones contained a fullsized insert of 1 kb. One unique human antiEGFR scFv (F4scFv) was isolated by analyzing with cell ELISA and DNA sequencing.

结果 经过5轮筛选，细胞裂解液中洗脱出噬菌体效价有500倍以上增长；细胞ELISA结果显示多克隆pscFv与CHOEGFRGFP1细胞和CHOK1细胞均有明显结合，但有部分特异性结合EGFR；菌落PCR显示约45％克隆中含有完整的1kb scFv片断；经细胞ELISA、 DNA测序检测共获得1株EGFR特异性单链抗体，命名为F4scFv。

This allows the crab to insert a large toothlike appendage on its right-hand claw into the opening, thus cracking the snail's shell.

这样就可以把它右爪那边象牙齿样的东西刺进蜗壳口从而弄碎蜗壳。

Methods In 19 patients with nonmalignant tracheoesophageal fistulas in intensive care unit, 9 patients accepted PEG tubes inserted by push technique. The remaining 10 patients accepted the insert of self-expandable metallic stents and the stents were extracted 2 months later with the complete mucosal healing confirmed by endoscopy and typical swallow-cough sign.

19例良性气管食管瘘患者，9例行PEG留置造瘘管、10例行内镜下食管覆膜自膨式金属支架植入术，2个月后经内镜将金属支架取出，以吞咽—咳嗽征检测气管食管瘘是否完全愈合。

My father is over 60 years old, and he lifts weights every day.

我爸爸六十多岁了，他每天都练习举重。

Then in a serious tone she declared:"It doesn't pay, dear boy, to look like a ninny with one's wife the first night."

两眼湿润了，她把身子蜷得很小，这样似乎可以更好地闻闻自己。