查询词典 insert cells

The hFCECs were cultured by sticking tissues piece method that the cornea were divided into the limbus and central tissue piece and digestive method,respectively.For digestive method,the digestive juice of 5mg/ml DispaseⅡ+0.25%trypsin was chosed.D/F12 with 10%fetal bovine serum and 100 U/ml penicillin and streptomycin were used in this study,the culture condition was 37℃and 5%CO2,and culture medium changed every three days2、Passage of hFCECsAfter more than 80%confluenced,cells from corneal limbus hFCECs were digested for 5-15min with different concentrations of trypsin/EDTA at 37℃,and then passaged at a ratio of 1:2.The cells were subcultured on empty plates,mouse 3T3 fibroblast feeder layer,fetal corneal stromal cell feeder l ayer or HTK feeder layer respectively.And D/F12, mouse 3T3 fibroblast conditioned medium,fetal corneal stromal cells conditioned medium or HTK conditioned medium with 10%FBS and 100 U/ml penicillin and streptomycin were used respectively as the culture medium.And the culture medium were changed every three days as well.

方法一、人胎儿角膜上皮原代和传代培养1、原代培养严格无菌操作获取人胎儿角膜片，采用组织块贴壁法、酶消化法原代培养人胎儿角膜上皮细胞。2、传代培养角膜缘部的细胞生长融合达80%以上，不同稀释浓度的胰酶/EDTA消化液消化传代分别接种于空板、小鼠3T3成纤维细胞饲养层、胎儿角膜基质细胞饲养层及HTK饲养层，培养液分别采用D/F12、小鼠3T3成纤维细胞条件培养液、胎儿角膜基质细胞条件培养液、HTK条件培养液。

So it is generally assumed that tumor cells are derived from normal stem cells, more work is needed to identify and characterize epithelial tumor stem cells.

然而，目前研究中关于上皮肿瘤干细胞及其与正常上皮干细胞的关系了解甚少。

Adult stem cells can break away restricted lineage and be trans-differentiated into other mature cells, this phenomenon is referred to as plasticity which is the base of clinical application of adult stem cells and has a promising future.

这些干细胞可以打破胚层界限，横向分化为其他组织的成熟细胞，这种性质称为干细胞的可塑性，是成体干细胞应用于临床的理论基础，具有广阔的临床应用前景。

Pue group is more formation of cartilage cells,cartilage cells,necrotic degeneration of the surface than the model group and the Sodium Chloride group with light rare exudation of inflammatory cells by microscope,we could see new blood vessels and fibroblasts.

光镜观察见葛根素组软骨细胞较平整，表层软骨细胞坏死变性较模型组和生理盐水组轻，炎性细胞渗出物少见，可见新生血管和成纤维细胞增生。

The formed elements consisting of red blood cells, white blood cells, squamous and non-squamous epithelial cells, sperms, yeasts, hyalin and non-hyalin casts, crystals (calcium oxalate, uric acid and triphosphate) were detected with UriSed automatic urine sediment analyzer.

应用朗迈UriSed型全自动尿沉渣分析仪对尿液中的红细胞、白细胞、上皮细胞、精子细胞、酵母菌、管型、结晶（草酸钙、尿酸、三磷酸盐）进行检测。

Studies on mouse nuclear transfer with adult male mouse ear fibroblast cells as donors In this study, C57BL/6 adult male mouse ear fibroblast cells and Kunming mouse MⅡoocytes were used as donors and recipients, respectively, to investigate the effects of different passages of donor cells and electrofusion times on the in vitro development of NT reconstructed embryos, when the oocytes were treated with 3% sucrose.

以成年雄鼠耳成纤维细胞为供体克隆小鼠的研究该研究以C57BL/6 成年雄鼠耳成纤维细胞为供体，昆明白小鼠MⅡ期卵母细胞为受体，研究了3%蔗糖处理卵母细胞去核后，供体细胞不同传代次数和电融合次数对核移植重构胚体外发育的影响。

Result: With a high cancer-inhibiting rate on the animal model having been transplanted the cancer, this medicine can remarkably restrain the growth of cancer cells cultivated in vitro and improve the life quality of mouse model, and also shows obvious promotion and enhancement function on the DTH ear-swelling of mouse with cancer, the proliferation of mouse spleen lymphocyte induced by ConA and the cytokine (TNF-α、IFN-γ) of mouse with cancer respectively. It can obviously decrease the adverse effects like weight reduction, damage of viscera and degradation of life quality caused by the chemotherapeutic medicines, and has a remarkable stimulation on the lymphocyte proliferation induced by ConA. It is also able to improve the ratio of SMMC-7721 cells in G0-G1 period and reduce the cell amount in G2/M period to cut down the possibility of stepping into the division period; additionally, it can obviously inhibit some cancer genes of the cancer cells and meanwhile up regulate the cancer-inhibiting gene.

结果：本品能明显抑制体外培养肿瘤细胞的生长，对在体肿瘤移植模型动物具有较高的抑瘤率，能明显提高模型小鼠的生存质量；对荷瘤小鼠DTH耳肿胀、ConA诱导的小鼠脾淋巴细胞增殖、荷瘤小鼠细胞因子（TNF-α、IFN-γ）呈现明显的促进或提高作用；能显著减少化疗药所引起的小鼠体重降低、脏器损害以及生存质量下降等不良作用；本品含药血清对ConA诱导的淋巴细胞增殖具有明显的促进作用；能提高SMMC-7721细胞G0-G1期比例，降低G2/M期细胞数目，而减少其进入分裂期；对肿瘤细胞的多个癌基因具有明显的抑制作用，同时上调抑癌基因等。

Vascular endothelial cells and smooth muscle cells provide the seed cells for vascular tissue engineering.

所培养的血管内皮细胞和平滑肌细胞为血管组织工程提供了细胞来源。

The book focuses on alkaline fuel cells, proton exchange membrane fuel cells and solid oxide fuel cells.

全书重点在碱性燃料电池、质子交换膜燃料电池和固体氧化物燃料电池。

The quantity of CD3+ cells in the EdNSC, AdNSC of cloning goattransplantation group and control group had no statistics difference(P＞0.05) at acute and chronic period.Overall, in our study, We got the conclusions as follow: 1,The EdNSC, AdNSCof cloning goat and the AdNSC of the non-cloning goat were successful obtained;EdNSC growed faster than AdNSC in the same cell culture method. 2,The braincontusion and laceration model of cloning goats were successful established by freefalling of 50 gram of steel ball accelerate strike on the goat brain from 100cm height.3, EdNSC and AdNSC transplantation both had obvious therapeutic effect on braincontusion and laceration of cloning goats; The therapeutic effect of EdNSCtransplantation was better. 4, Allograft of stem cells has acute and chronicimmunological rejection and autografting of stem cells has no acute and chronicimmunological rejection.

在相同的条件下，EdNSC体外培养速度比成AdNSC快。2对羊脑上薛氏沟周围运动皮层区使用50g×100 cm力度作自由落体加速撞击，可产生观察指标明确且存活率高的羊脑挫裂伤模型，有望广泛用于神经干细胞移植及药物等治疗的实验研究。3胚胎神经干细胞及成体神经干细胞移植治疗脑挫裂伤后均有较明显的治疗效果；二者相比较而言胚胎神经干细胞稍好于成体神经干细胞。4中枢神经系统异体神经干细胞移植存在着明显的免疫排斥反应，而自体神经干细胞移植基本无免疫排斥反应

A strange and mysterious deep sea creature.

一种奇异、神秘的深海生物。

It's a yellow rectangle .

这是黄色的长方形。