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This result reveal that the cell is pancreatic stem cells and pancreatic stem cells do exist in pancreas,It maintains the steady of pancreas by neogenesis and differation. At present,our laboratory can isolate and expand this cell steadily.This study can provide reference for isolation and expansion of pancreatic stem cells.
说明胰腺内存在着多潜能干细胞,本方法分离到胎猪胰腺干细胞,经诱导分化可形成有功能的胰岛样细胞团并释放胰岛素,有望为进一步研究异种胰腺干细胞移植治疗糖尿病提供材料。
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To study its location in other nephron segments and its role in kidney function, we performed immunohistochemical analysis and found that SUCNR1 is located in the luminal membrane of macula densa cells of the juxtaglomerular apparatus in close proximity to renin-producing granular cells, the cortical thick ascending limb, and cortical and inner medullary collecting duct cells.
为了研究其在肾单位的分布及其对肾功能的影响,我们采用了免疫组化的方法,发现 SUCNR1位于与产生肾素的颗粒细胞相邻球旁器的致密斑细胞腔内膜上、皮质升支粗段、皮质及髓内集合管细胞上。
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PC12 cells were exposed to RA with different concentrations for 72 h and the lengh of neurite and max diameter of PC12 cells were observed at 24,48,and 72 h respectively. The expression of MAP2 was detected by immunocytochemistry after PC12 cells were exposed to RA for 72 h.
不同浓度的RA组均诱导PC12细胞72 h,在24 、48 和72 h分别观察细胞的突起长度和细胞最大直径的变化情况;在诱导72 h后利用免疫细胞化学技术,观察不同剂量RA作用后PC12细胞向MAP2阳性细胞分化的情况。
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PC12 cells were exposed to NGF with different concentrations for 72 h and the lengh of neurite and max diameter of PC12 cells were observed at 24,48,and 72 h, respectively. The expression of MAP2 was detected by immunocytochemistry after PC12 cells were exposed to NGF for 72 h .
不同浓度的NGF组均诱导PC12细胞72 h,在24、48和72 h分别观察细胞的突起长度和细胞最大直径的变化情况;在诱导72 h后利用免疫细胞化学技术,观察不同剂量NGF作用后PC12细胞向MAP2阳性细胞分化的情况。
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The expression of CD40, the infiltration of inflammatory cells and the distribution of Langerhans' cells in the skin of neurodermatitis patients was investigated by analyzing the distribution of Langerhans' cells and CD40 expression in injured skins of different degrees of inflammatory, infiltration at different stages of the disease by immunohistochemical mathod.
通过分析神经性皮炎组织病理变化、炎性细胞浸润、朗格汉斯细胞的分布以及CD40分子的表达,探讨这些变化与临床分型及病程的相关性。应用常规病理切片分析以及免疫组化的方法,观察LC和CD40分子在不同病期和炎症浸润程度皮损中的分布和表达情况。
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Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.
结果 (1)ATP对U937细胞的增殖有按摩明显的阻抑作用,加药48h后增殖的抑制率可达43%以上;(2)ATP处理的U937细胞周期发生改变,G1期细胞数按摩明显增多,ATP作用24h时G1期前出现亚二倍体峰——凋亡峰,凋亡细胞数为3.4%,作用48h时凋亡细胞数增多为22.7%;(3)ATP处理的U937细胞首先在核内染色质浓缩成半月形贴近核膜,逐渐向核膜外移动,进入胞浆内再移向质膜内外面,紧贴质膜外面再逐步脱离细胞体,进入细胞基质中,成为游离的凋亡小体。
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Methods Cell culture,flow cytometry,HE staining,Nigrosine staining and electron microscopy were used. Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.
结果 (1)ATP对U937细胞的增殖有明显的阻抑作用,加药48h后增殖的抑制率可达43%以上;(2)ATP处理的U937细胞周期发生改变,G1期细胞数明显增多,ATP作用24h时G1期前出现亚二倍体峰——凋亡峰,凋亡细胞数为3.4%,作用48h时凋亡细胞数增多为22.7%;(3)ATP处理的U937细胞首先在核内染色质浓缩成半月形贴近核膜,逐渐向核膜外移动,进入胞浆内再移向质膜内外面,紧贴质膜外面再逐步脱离细胞体,进入细胞基质中,成为游离的凋亡小体。
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Ore-binding factor alpha 1 plays an essential role in bone formation and osteoblast differentiation and serves as a master gene regulating osteoblast-specific gene expression. The gene is expressed in cells of the osteoblast lineage only, and when expressed in non-skeletal cells, the cells assume many of the characteristics of an osteoblast.
bfal在骨形成和成骨细胞分化过程中起着重要的作用,是调控成骨细胞特异性基因表达的主控基因,该基因只在骨形成相关细胞中表达,如果使其在非成骨细胞中表达,该细胞将出现成骨细胞的特征。
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An ommatidium composed of cornea, two corneagenous cells, four cone cells and eleven retinular cells.
复眼除背面有一拇指状的区域无小眼外,其余部分都由小眼组成。
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5Um〓. The surface area of the compound eye is about 21. 044mm〓. An ommatidium composed of cornea, four corneagenous cells, four cone cells and retinular cells.
光镜观察显示红螯螯虾复眼的每个小眼从远端到近端分别由角膜、4个角膜生成细胞、4个晶锥细胞组成的晶锥及小网膜细胞构成。
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- 推荐网络例句
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Objective:To compare the response control and attention of Schizophrenic patients with that of the healthy controls by Integrated Visual and Auditorycontinuous performance test. To quantitate the impairment of cognitive function in patients, and to explore the relationship between cognitive function and the severity of the disorder.
目的:探讨精神分裂症病人在IVA持续操作测试中的反应控制能力、注意力等,并与健康人进行比较,从而量化精神分裂症病人的认知功能损害,并进一步探讨其认知功能与疾病严重程度的关系。
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Main effective factors including subcooling degree, mechanical vibration, gas hydrate reformation, environment temperature, noncondensing gas and surfactant are analyzed.
指出过冷度、机械振动、重复生成水合物、环境温度、不凝性气体、添加剂是影响气体水合物生成的主要因素,还对R152a水合物的放冷进行了实验研究。
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Water is the construction and development of socio-economic basis,and strategic resources.
水是社会经济建设与发展的的基础性,战略性资源。