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Methods Hepatoma carcinoma cells and pulmonary carcinoma cells were cultured in vitro, Mononuclear cells in healthy person's peripheral blood were collected. PBMCs were mixed with liver cancer and lung cancer cells by proportion, and enriched tumor cells by superparamagnetic nanoparticle antibody complex, lung cancer specificness was identified by fluorescence nanocrystals bioprobe. We smeared tumor cells on glass slide and identified pulmonary carcinoma cells specfficity by fluorescence nanocrystals bioprobe.

体外培养人肝癌细胞和肺癌细胞，采集健康人外周血单个核细胞，将PBMC与肝癌细胞和肺癌细胞按比例混合，通过磁粒抗体复合物富集癌细胞，用荧光纳米晶探针进行肺癌特异性鉴定。

At postnatal 1st week immunopositive reaction of NGF was detected mainly in sustentacular cells and the spermatogonia also showed positive staining. NGF positive staining in the testes was observed in interstitial cells, spermatogenetic cells, sustentacular cells and Leydig cells at 3rd week. After the postnatal 5th week, NGF-positive immunostaining was also detected in intersitial cells and spermatogenetic cells, but the intensity of reaction was weaker than that at 1st and 3rd weeks.

免疫组化定位分析显示：睾丸组织的神经生长因子蛋白表达于小鼠出生后的各个时期内，1周龄睾丸组织免疫阳性反应主要位于支持细胞，精原细胞也有着色；3周龄睾丸组织的间质细胞、各级生精细胞、支持细胞、管周肌样细胞表达均呈现阳性；5周后的睾丸组织内神经生长因子呈低水平表达，主要表达于间质细胞和生精细胞内。

RESULTS: APC promoter 1A was methylated in NCI-H460 cells, and unmethylated in NCI-H446 and SPC-A1 cells. Hypermethylation was detected in all 5 CpG islands (687, 707, 714, 719, 726) of APC promoter 1A in NCI-H460 cells. The expression of APC in NCI-H460 cells was decreased by 26.04% of that in NCI-H446 cells and by 32.36% of that in SPCA1 cells. After treatment of 1, 5, 10, 15μmol/L 5-aza-dC, the expression of APC promoter 1A in NCI-H460 cells was enhanced by 4.59, 5.78, 9.58, 5.98 folds, respectively.

结果：SPC-A1和NCI-H446细胞APC甲基化阴性，NCI-H460细胞APC甲基化阳性；甲基化芯片检测Ncl-H460细胞在APC启动子1A 5个CpG位点均存在甲基化(687、707、714、719、726)，SPC-A1和NCI-H446甲基化阴性，荧光定量结果NCI-H460的APC转录较SPC-A1和NCI-H446有明显的下降，仅为二者平均的30.04%；经5-aza-dC脱甲基化作用后，NCI-H460细胞的APC表达增加了约5~10倍，其中10μmol/L浓度作用下，APC表达增加最多。

Type II cells were mainly distributed in layer V of cortex. It appeared that these cells receive the CRF immunostaining fibers terminals afferent from other cells rather than synthesize CRF by themselves. It is probable that type II cells receive afferent fibers from both extracortex and type I cells within layers II -III cortex. Considered that Type I cells morphologically are inhibitory interneuron, we presumed that type I in layer II -III could inhibit activity of type II cells in layer V.

CRF阳性Ⅱ型细胞主要分布在皮层第Ⅴ层，形态学特征显示这类细胞更像是接收来自其它神经元的CRF纤维投射而非自身分泌CRF，证据显示Ⅱ-Ⅲ层CRF神经元纤维可进入第Ⅴ层，考虑到皮层的CRF神经元形态上为抑制性中间神经元，这些结果提示Ⅱ-Ⅲ层CRF神经元能够抑制第Ⅴ层神经元的活动。

Results There was a similar distributive pattern of Neul, PPCA and β-gal in the inner ear. Neul intense staining was observed in the cochlear spiral ganglion cells, spiral limbus, spiral ligament, vestibular ganglion cells, cristae, maculae hair cells, and weak staining in inner hair cells, outer hair cells, supplying cells of the organ of Corti and stria vascularis. The intense staining of PPCA and β-gal were observed in the spiral ganglion and vestibular ganglion cells, and weak staining in the spiral limbus, spiral ligament, stria vascularis and organ of Corti. The inner ear exhibited no staining when Neul, PPCA and β-gal were deficient, respectively.

Neul最强的染色主要在螺旋神经节细胞、螺旋韧带、螺旋缘、前庭神经节细胞及壶腹嵴、球囊和椭园囊感觉毛细胞，较弱的染色分布于血管纹和Corti器内、外毛细胞及支持细胞；PPCA和β-gal在螺旋神经节和前庭神经节细胞有较强的染色，血管纹、螺旋韧带、螺旋缘和Corti器内、外毛细胞及支持细胞呈较弱的染色反应；各自酶缺乏时内耳免疫染色消失。

Results (1) Cells in frontal recess: The existence of terminal cells was 45.5% in frontal sinusitis group and 23.7% in, control; anterior ethmoid cell 31.8% and 15.3%; agger nasi cells 28.8% and 13.5%.(2) Cells in frontal sinus: for perifrontal cells there were 42.4% and 22%, respectively; superaorbital cells 33.3% and 25.4%; intersinus septal cells 27.3% and 20%.

①额隐窝内出现的相关气房（无额窦炎组出现率／额窦炎组出现率）：终末气房(23.7%/45.5%)，前筛气房(15.3%/31.8%)，鼻丘气房(13.5%/28.8%)；②额窦内出现的相关气房：额气房(22%/42.4%)，眶上气房(25.4%/33.3%)，额窦中隔气房(20%/27.3%)。

Results were shown as followings:(1) Sodium selenite at 0～2.5 μmol/L significantly increased the antioxidative capacity of L-02 cells without having remarkable impact on SMMC-7721 cells;(2) Sodium selenite at concentrations above significantly increased telomerase activity, hTERT gene expression and telomere length of L-02 cells without significant impact on SMMC-7721 cells;(3) Sodium selenite at higher concentrations (larger than 5 μmol/L) resulted in peroxidation of L-02 cells, while scutellarin significantly counteracted its effect;(4) Selenium-rich amino acids from silkworm pupas in the range of 0.5～2.5 μmol/L Se significantly inhibited SMMC-7721 cell growth, induced apoptosis and cell cycle change, and the generation of reactive oxygen species. In contrast, sodium selenite and selenomethionine only had weak impact on them at the same concentrations;(5) A new selenium-containing protein was found from selenium-rich silkworm pupas, which is worthy to be study further;(6) An expression vector containing ansense RNA of hTERT gene were constructed and used to transfect SMMC-7721 cells. They were observed to inhibit hepatoma cells.

结果如下：（1）0～2.5μmol/L亚硒酸钠显著性增强L-02细胞的抗氧化能力；而对SMMC-7721细胞的作用不显著；（2）该浓度硒显著性提高L-02细胞端粒酶活性、增强hTERT基因表达和延长细胞端粒长度；而对SMMC-7721细胞的作用均不显著；（3）高浓度硒（5μmol/L以上）显著性抑制L-02细胞生长、致细胞过氧化，灯盏花素能拮抗硒所致过氧化、降低硒毒性；（4）0.5～2.5μmol/L富硒蚕蛹氨基酸显著性抑制肝癌细胞SMMC-7721生长、导致细胞凋亡和周期改变、诱导细胞产生活性氧，同浓度亚硒酸钠和硒代蛋氨酸对其抑制不显著；（5）富硒蚕蛹蛋白经分离纯化和鉴定后发现存在一新含硒蛋白，其结构和功能有待研究；（6）通过已有的含hTERT基因质粒，成功构建hTERT反义RNA表达质粒，转染SMMC-7721细胞后对其生长具有抑制作用。

The morphology and structure of reconstructed tissue was detected by microscope and scanning electron microscope.Results:(1) Compared with the control group, the cellular proportion of laminin group increased in 62 ~M phase, and decreased in Go~Gi phase significantly. As shown by the microscope, the cells of control group were in low density. The cells in mass connected tightly. The microfilament appeared reticular formation. The nucleus were the same in size. The cells of laminin group were in high density and put out so many lamellipodia, filopodia, which connected with the surrounding cells. The microfilament increased, elongated, and changed from reticulodromous to sarciniform, which reached to the pseudopods. The nucleus were different in size .(2) As shown by the inverted microscope and the cell growth curve, comparing with the controlgroup, cells of each test group increased evidently. The cellular proportion of each test group increased in S phase and G2 ~M phase, and decreased in Go~Gi phase significantly, but there was no considerable interations between LN and EGF;(3) As shown by the morphological observations, the cultured cat corneal endothelial cells formed an integrated membrane, and attached to the Descemets membrane closely, which was similar to the natural tissue. The cells connected tightly to each other, and some of them arranged in hexagon approximately.

结果：(1)层粘连蛋白组处于G_2～M期细胞比例较对照组显著提高，Go～G_1期细胞比例显著下降，提示层粘连蛋白促进内皮细胞DNA合成，及细胞分裂增殖；光镜下，对照组细胞分布成团状，细胞密度较低，细胞间连接紧密，细胞内微丝结成网状，细胞核大小一致；与对照组相比，层粘连蛋白组细胞生长旺盛，细胞密度高，向周边伸出大量板状及丝状伪足，细胞内微丝增多、拉长、集结成束，伸入伪足中，细胞核形状大小不一致；(2)倒置显微镜观察及细胞生长曲线显示，各组细胞数目随时间增加而明显增多，各实验组较对照组增生显著，EGF和LN联合应用组各时间点细胞数目最高；实验组处于S期和G_2～M期细胞数目增加，Go～G_1期细胞数目减少；提示EGF、LN单独及联合应用均可促进细胞增殖，但尚不能认为二者有交互作用；(3)倒置显微镜下，组织培养的猫角膜内皮细胞排列成密集的单层，细胞间连接紧密；组织学观察发现，培养的猫角膜内皮细胞形成完整的内皮层，贴附于脱水基质的后弹力膜上，与正常的角膜内皮组织结构相似；扫描电镜下，组织培养的猫角膜内皮细胞间紧密镶嵌排列，可见某些细胞呈近似六边形排列，细胞大小不甚一致，胞核清晰。

You will come in May between June, between choice section shorter give birth to half lignification branch in those days, from base the ministry cuts dirty to insert spic, topmost reservation comes 3 pieces 5 flocculus, if branch is too long, yi Ke bite off carries a tip on the head, cuttage medium can use humus soil of orchid mud, high mountain, vermiculite, pearlite or Huang Xin earth to wait, thick about 15 centimeters reach 20 centimeters, the catchment layer that lower berth reachs 8 centimeters 7 centimeters; medium after in season is wet, in will inserting spic to insert matrix, deepness of the be buried that insert spic makes an appointment with the 1/2 that grows for spic, a few cuttage also can choose the family full-dress medium generation makes big flowerpot cuttage seedbed.

你可于5月至6月间，选择节间较短的当年生半木质化枝条，自基部切下作插穗，顶端保留3片至5片小叶，若枝条过长，亦可截去顶梢，扦插介质可用兰花泥。高山腐殖土。蛭石。珍珠岩或黄心土等，厚约15厘米至20厘米，下铺7厘米至8厘米的排水层；把介质喷湿后，将插穗插入基质中，插穗入土深度约为穗长的1/2，家庭少量扦插也可选用大花盆盛装介质代作扦插苗床。插后加盖塑料薄膜保湿，温度控制在25℃至30℃之间，加设荫棚遮挡阳光，或挪放于树荫下，始终保持扦插基质湿润，东鹃约过1个月即可生根，而西鹃则要60天至70天才能生根。9月后可减少遮荫时间，并追施稀薄尿素液一次，浓度约为0.2%，10月下旬即可上盆。

The method of producing the pulley assembly may include modifying as by knurl (22 the outer flat circumferential surface of the metal insert prior to molding the plastic annular body about the metal insert, for clamping, structurally bonding, and resisting relative rotation between the plastic body and the metal insert.

生产滑轮组件的方法可以包括：在金属插入件周围模制塑料环形体之前，改变金属插入件的外圆周表面（例如通过压花（22）），以夹紧、在结构上连接及防止塑料体和金属插入件之间的相对转动。

"Second Life is remarkably easy to work with, and is very popular,"

"第二次生命是显着容易的工作，并且很受欢迎，"

For example, we usually assume that materials are homogeneous and isotropic and free of internal defects or flaws.

为了得到适合有限元分析的模型，我们必须经过如图2所示的简化步骤。