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Moreover, monkeys inoculated? with the nef free SIV developed marked resistance to the more virulent strain.

而且，猿猴接种过已去除&否因&的SIV后，会发展出明显的抵抗力，可以抵抗毒性强的SIV品种。

To prove the capability of 042BS to nodulate both soybean and alfalfa, constitutively expressed green fluorescence protein gene (gfp carried by pMP2444) was introduced to 042BS, and the recombinant strain 042BSG was obtained. The reisolates from nodules of the soybean and alfalfa inoculated with 042BSG were observed using the confocal laser-scanning microscope, and the expressions of gfp were detected, respectively.

将其接种保定苜蓿和北引1号大豆，并重新分离出根瘤菌，在激光共聚焦荧光显微镜下检测，可以清楚地观察到标记基因的表达，从而确证了042BS能同时在苜蓿和大豆上结瘤。

After the selection from one to three generations, several mutants were obtained. The major mutants included non-nodule mutants (18 lines) which failed to nodulate after inoculated with rhizobia for two weeks, ineffective nodulation mutants (9 lines) which had less uneven and white or transparent nodules, an abnormal leaf and flower mutant which was dwarf without stipules, abnormal flowers and less nodules, and a slender mutant with a thin stem and smaller leaves.

从种植的三代植株中，筛选到多种共生固氮根瘤的突变体，其中不结根瘤突变体18个株系，表现为接种根瘤菌两周后无根瘤形成；结无效根瘤突变体9个株系，表现为根瘤数目少且分布不均匀，根瘤呈白色，有些为半透明；花叶形态异常突变体1个株系，表现为除根瘤数目少外，植株矮小、托叶消失、花形态异常；纤细突变体1个株系，表现为除根瘤数目少外，植株变小、茎细叶小。

Many factors may be involve in the course. To investigate the regulation activity of mesenchymal cells to differentiation of epithelial cells from hair follicle and to study its differentiation property, mesenchymal cells gel was made by nubby dermal papilla cells, free dermal papilla cells, skin fibroblasts. Skin keratinocytes and epithelial cells from hair follicle were inoculated on the gel surface and cultured in air-liquid interface. Three-dimensional model of DPC using to induce epithelial cells differentiation is built in vitro.

为了进一步研究毛囊细胞间的相互作用，探讨毛囊间质细胞对毛囊上皮细胞分化的调节作用，研究毛囊上皮细胞的分化特性，我们利用团块状的毛乳头细胞，游离分散的毛乳头细胞或皮肤成纤维细胞制成间质细胞胶原凝胶，表面接种皮肤角质形成细胞或毛囊上皮细胞，进行气-液界面培养，在体外建立了毛乳头细胞诱导毛囊上皮细胞分化的立体模型。

Induced by 4℃ low temperature, Escherichia coli inoculated in water can enter the viable but nonculturable state; in the room temperature, Escherichia coli may keep culturable state for a long period; eutrophic culture media of TSA can produce more bacteria count compared with oligotrophic culture media of PCA; evaluating the bacteria count Escherichia coli in water by plate count method may underestimate the risk of intestinal pathogen contamination.

在4℃低温诱导下，大肠埃希菌在水中可进人活的非可培养状态；在室温下，大肠埃希菌在水中可保持较长时间的可培养性；富营养的TSA琼脂检测的菌落数多于寡营养的PCA琼脂；用平板涂布法检测水中的大肠埃希菌可能低估了肠道致病菌污染的风险。

Methods Nail samples were collected from patients with suspicious onychopathy, and inoculated into modified DTM, DTM, Sabouraud dextrose agar fortified with cycloheximide and chloromycetin and Sabouraud dextrose agar fortified with chloromycetin respectively. The initial time of fungal growth and of discoloration of medium, as well as the colony diameter at the beginning of discoloration, were documented.

收集临床拟诊甲真菌病的标本，分别接种于改良DTM、DTM、含放线菌酮及氯霉素的沙氏培养基和含氯霉素的沙氏培养基；记录菌株的开始生长时间、培养基开始变色时间及开始变色时菌落的直径。

Through DOT-blot hybridiztion the result indicated SA215 and SA215 were of the same distribution on animal ,simply planting the following trigeminal ganglion .21-day-old SPF pigs inoculated intramuscularly SA215 strain could be protected from being affected at day 28 after oronasal challenge-exposure with 106 PF.U high-dose virulent PRV Fa strain and at day 42 after i.m.

SAZ巧株lml接种21日龄健康仔猪(伪狂犬病和猪瘟检测阴性)，在接种后28天用10毕FU的PRV Fa强毒株进行滴鼻攻毒，42天用猪瘟强毒SM株血毒lml肌肉注射攻击，结果表明接种猪能抵御两次病毒攻击，而同条件下对照结果成立，表明SAZ 15株具有良好免疫原性。

We observed that cyclophosphamide-immunosuppressed adult mice were Susceptible to acute infection leading to death.4-8 week old C57 mice or outbred Kunming strain mice inoculated in-tracerebrally with HFRS virus A-16 strain and immunosuppressed with four 50mg/kg CY doses at days -1,+1,+ 2,+ 4 (day O:virai infection), developed a lethal disease94% m...

在第-l、+1，+2、+4天注射环磷酰胺50mg/kg体重(第0天感染病毒)，可使小鼠由隐性感染变为急性致死性感染，病死率为94％。在病死动物的脑，肺、肝、脾和肾等器官中，检查翻HFRS病毒抗原。这些器官显示微血管系统和实质组织的病变。这种成龄鼠模型可用于本病的研究。

Fourth, the liquid state seed of SM12-87 was inoculated into the solidfermentation substrate of Shanxi overmature vinegar by 3% inoculums size.

由此看来只以一半醋醅进行熏醅、淋醋，另一半直接淋醋，然后混合的传统熏醅工艺具有合理性。

The poplars inoculated by overwinter urediniospores could result in diseases.

杨树锈病在我国广泛分布于东北、华北、西北、西南和华东等地区。

A historical region of southwest France between the Pyrenees and the Garonne River. The duchy of Aquitaine was joined with France after the marriage of Eleanor of Aquitaine to King Louis VII in1137, but its possession was disputed after her subsequent marriage to Henry II of England.

阿基坦法国西南部一个历史地区，位于比利牛斯山和加隆河之间，在1137年阿基坦的埃莉诺与国王路易斯七世结婚之后阿基坦公国加入法国，但当她再嫁英国亨利二世后，其归属权便受到争议

There are moments when I look at the smiling , happy faces of my expectant staff and wonder how they will cope when confronted by an outraged housemaster along with an unrepentant and recalcitrant bleached blond teenager in 18 years ' time .

有时候，我看着那些满怀期待的员工微笑，幸福的脸，想着当他们遇到一位愤怒的宿舍管理员和一位不知悔改，叛逆，而且将头发染成白色的18岁少年时，他们会如何处理。