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In this article, the method for detecting five kinds of biogenic amines by HPLC was established, and the contents of biogenic amines in Chinese rice wines and soy sauces were detected. In order to reduce the contents of BA, we inoculated Lactobacillus curvatus 6031 and Leuconostoc mesenteroides 51-5 D in sausage and the contents of BA and the microbial counts was studied during the fermention of sausage.

建立了高效液相色谱测定生物胺含量的方法，方法稳定性好、精密度高，回收率高，五种生物胺的回归方程分别为：y 尸胺=48.903x R 2=0.9996；y 组胺=36.105x R 2=0.9994；y 酪胺=29.865x R 2=0.9902；y 亚精胺=46.671x R 2=0.9967；y 精胺=31.239x R 2=0.9926，在2～40μg/ml的范围内R均大于0.99，检测限分别为：尸胺、组胺和亚精胺为0.05μg/ml，酪胺为0.1μg/ml，精胺为0.25μg/ml。

Method\ Yersinia pestis (strain No.47004) isolated from Marmota himalayana were inoculated subcutaneously in the dose of 1×10 1～1×10 9 viable bacill into M.meridianus capt...

用分离自喜马拉雅旱獭体的鼠疫菌 470 0 4号，以 1× 10 1 ～ 1× 10 1 0 个菌皮下接种从塔里木盆地捕获的子午沙鼠。

Using polyacrylamide gel electrophoresis, the peroxidase isoenzyme of three different resistant varieties of apple seedlings inoculated with Marssonina coronaria was studied.

应用聚丙烯酰胺凝胶电泳，测定抗性不同的3个苹果品种过氧化物酶同工酶在接种褐斑病菌后的变化规律。

The cooled wort is inoculated with Saccharomyces yeast and fermentation of the sugar formed from starch during mashing proceeds.

向冷却后的麦芽汁中注入酵母菌，使糖化过程中淀粉降解产生的糖发酵。

Conclusions】 It is suggested intramuscular injection is an effective immune route. Mice inoculated with coding different stage gene recombinants alone or mixedly could all induce increased humoral and cellular immune response, and NK cell activity.

提示肌肉注射为一有效的DNA疫苗免疫途径，采用编码恶性疟原虫有性期阶段或无性红细胞阶段的重组质粒单独或两者混合免疫小鼠，均能诱导明显的体液免疫反应、细胞免疫反应和NK细胞杀伤活性。

When reached logarithm growth phase, it was poured or the medium was removed. 5 mL medium containing 10 mg/L mitocin-C was added , kept at 37 ℃, and then cultured in saturated humidity warm box with the CO2 of 0.05 fraction volume for 2 or 3 hours. It was coated with 0.1% gelatin in 6-hole plate, laid for over 30 minutes, and then threw away or the medium with mitocin-C was removed, washed with phosptat buffer for 5 times so as to remove the mitocin-C. 2 mL 0.05% trypsin digestive cells were added, and it was observed under microscope. When crevice appeared, cells became round (about 2－4 minutes), digestion was stopped by adding medium of the same volume, and blew up with straws repetitively to make it into monoplast suspension. Special-used cover glass was put in the center of cell counting chamber. Cells were sucked in by glass siphon, and cell suspension flew out at bucket of up or down-sides of counting chamber, until the cover glass was filled with fluid. Living cell were inoculated at concentrations of 3×108, 5×108, 1×109 L－1 after their number counted.

选取2～5代的小鼠胚胎成纤维细胞，待其至对数生长期倒掉或吸掉培养基，加入含10 mg/L丝裂霉素C的细胞全培养液5 mL，置37 ℃，体积分数0.05的CO2饱和湿度温箱中培养二三小时，在6孔板中加入0.1%明胶包被，放置30 min以上，倒掉或吸掉含丝裂霉素C的培养基，磷酸盐缓冲液清洗5遍，尽量除去丝裂霉素C，加入2 mL 0.05%的胰蛋白酶消化细胞，镜下观察，当细胞间出现裂隙，细胞变圆时（约2～4 min），立即加入等量的全培养液终止消化，并用吸管反复吹打，使之成为单细胞悬液，在细胞计数板中央放置专用的盖玻片，用玻璃虹吸管吸取细胞，让虹吸管在盖玻片上或下侧的计数板凹槽处流出悬液，至盖玻片下被液体充满为止。

After cooling, each bottle was inoculated with a 7 mm mycelia mat of 5-day-old actively growing culture of P.

在冷却之后，每支瓶子与 7 公厘 mycelia 垫一起接种 5 天的－旧的积极地＆ndash；P 的增加文化。

Edible or medicinal mushroom mycelia are inoculated and cultured in solid media made of grains.

在谷粒制成的固体培养基中接种和培养可食或有医药用途的蘑菇菌丝体。

Methods The Kunming mouses were inoculated with blood from the Mycoplasm suis infected pigs, the rate of infection and histopathological changes were determined in different stages after challenge.

用感染了附红细胞体的猪血液感染昆明小鼠，定期进行感染率和病理组织学检测。

Moreover, monkeys inoculated with the nef free SIV developed marked resistance to the more virulent strain.

而且，猿猴接种过已去除&否因&的SIV后，会发展出明显的反抗力，可以反抗毒性强的SIV品种。

A historical region of southwest France between the Pyrenees and the Garonne River. The duchy of Aquitaine was joined with France after the marriage of Eleanor of Aquitaine to King Louis VII in1137, but its possession was disputed after her subsequent marriage to Henry II of England.

阿基坦法国西南部一个历史地区，位于比利牛斯山和加隆河之间，在1137年阿基坦的埃莉诺与国王路易斯七世结婚之后阿基坦公国加入法国，但当她再嫁英国亨利二世后，其归属权便受到争议

There are moments when I look at the smiling , happy faces of my expectant staff and wonder how they will cope when confronted by an outraged housemaster along with an unrepentant and recalcitrant bleached blond teenager in 18 years ' time .

有时候，我看着那些满怀期待的员工微笑，幸福的脸，想着当他们遇到一位愤怒的宿舍管理员和一位不知悔改，叛逆，而且将头发染成白色的18岁少年时，他们会如何处理。