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Air sparging is widely used for remediating groundwater contamination of solvents or gasoline fuels. Air is injected into the saturated zone in the plume area through sparging well. Injected air contacts the contaminated groundwater and removes volatile organic compounds by volatization.

气提法应用在地下水及土壤污染整治的原理是将空气由注气井打入受污染区域的饱和含水层，注入地下水层的空气与非水相的挥发性有机污染物接触，会将污染地下水层的挥发性有机物挥发出来，并往上流入非饱和层中，再由抽气井抽出非饱和层之气体，将污染的挥发性有机物回收到地表进行处理。

In allusion to the corrosion of different injected water sources to the steel part of line and pump and so on in the processof oilfield waterflood development, the corrosion of dissolved oxygen, pH value, sulfureted hydrogen and salinity in the injected waterto steel was experimented and researched by the test of measuring average corrosion percentage in the laboratory.

针对油田注水开发中不同注水水源对管线、泵等钢材质部位的腐蚀，在实验室通过平均腐蚀率测定试验，对不同水源中的溶解氧浓度、pH值、硫化氢含量和盐的矿化度等因素对钢材的腐蚀进行试验研究。

The liver glycogen of big mice (injected with Astragals membranaceus or Rhodiola and not injected with drug) both non-exposed and exposed to noise were determined.

测定未经过噪声暴露和经过噪声暴露(未注射药物、注射黄芪或注射红景天)的大鼠的肝糖原含量，研究了噪声对大鼠肝糖原含量的影响及其药物防治。

The liver glycogen of big mice (injected with Astragals membranaceus or Rhodiola and not injected with drug) both non-exposed and exposed to noise were determined.

测定未经过噪声暴露和经过噪声露（未注射药物、注射黄芪或注射红景天）的大鼠的肝糖原含量，研究了噪声对大鼠肝糖原含量的影响及其药物防治。

The liver glycogen of big mice (injected with Astragals membranaceus or Rhodiola and not injected with drug) both non-exposed and exposed to noise were determined.

摘 要：测定未经过噪声暴露和经过噪声暴露(未注射药物、注射黄芪或注射红景天)的大鼠的肝糖原含量，研究了噪声对大鼠肝糖原含量的影响及其药物防治。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

The fusion DNA fragments of ag85b-mpb64 and ag85b-mpb64-esat-6 were obtained by PCR andSOE technique. Various DNA vaccines were constructed with the pcDNA3.1: fusion of two genes, and of three genes, bivalent combinations and trivalent combinations(pCA+pCM+pCE6). BALB/c mice were vaccinated with this DNA vaccines.The mice injected withBCG were positive control and the mice injected with pCDNA3.1 and PBS were negative control.The mice were immunized 3 times with 2-wk intervals. The animals in group BCG were only inoculatedsubcutaneously with 1×10~6 CFU BCG at initial vaccination. The serum IgG titers and IgG isotype weredetermined using iELISA coated with M. bovis PPD and rMAE protein expressed and depurated inprokaryotic expression system every week.

同样，利用PCR和SOE技术，获得牛分枝杆菌mpb64-ag85b和mpb64-ag85b-esat-6融合基因，以pCDNA3.1为载体构建了牛分枝杆菌多价组合和多基因融合DNA疫苗：二基因融合(pCDNA3.1-MPB64-Ag85B，简称pCMA)和三基因融合(pCDNA3.1-MPB64-Ag85B-ESAT-6，简称pCMAE)DNA疫苗；二价组合和三价组合(pCA+pCM+pCE6)DNA疫苗，免疫BALB／c小鼠，以牛分枝杆菌BCG免疫组为阳性对照，以pCDNA3.1及PBS免疫组为阴性对照，共免疫3次，每次间隔2周，BCG组仅初免时皮下免疫1次。1免后每周，以原核表达纯化的重组MPB64-Ag85B-ESAT-6蛋白和牛分枝杆菌PPD为包被抗原，以间接ELISA方法检测血清IgG水平及lgG亚类。

3 Mill in fine raw materials should be put in after the first injected fuel injected powder, and should be put at the turn of the harsh fat, cocoa liquor should be pre-cut into small pieces, heating dissolved (temperature not exceeding 50 ℃) may invest .

4.3精磨机在投放原料时应先投放油料后投放粉料，并应分次交替投放苛刻脂，可可液块应预先切成小块，加热溶解（温度不超过50℃）后方可投放。

objective to investigate whether hypoxia preconditioning exist in adipose tissue,and construct the hypoxemic model of adipose tissue.methods after injected different density cocl2 to rat's subcutaneously of groin,use immunohistochemical method detect hif-1α protein,and use rt-pcr detect hif-1α mrna.results after injected different density cocl2,the adipose tissue of rat's groin express different degree of hif-1αprotein and hif-1αmrna.,and reach the peak when the density is 20-40 mg/kg and the time is 3 h.conclusion hypoxia preconditioning exist in adipose tissue,and the effect of preconditioning is most evidence when the density is 20-40 mg/kg and the time is 3 h.

目的 探讨脂肪组织中是否存在缺氧预适应现象，构建cocl2致缺氧的缺氧模型。方法不同浓度的cocl2大鼠腹股沟区皮下层浸润注射，分别用免疫组织化学法和rt-pcr法检测该区脂肪hif-1α蛋白和hif-1α mrna的表达情况。结果用不同浓度的cocl2行化学缺氧后在不同时间点观察有不同程度的hif-1α蛋白和hif-1α mrna表达，其中，在浓度为20～40 mg/kg，时间为3 h时脂肪组织中hif-1α表达达高峰。结论脂肪组织存在缺氧预适应现象；cocl2的浓度选择20～40 mg/kg，时间选择3 h时缺氧预适应效果较佳。

It has to be computed by the host for the actual clock rate.

主控制器可以用来计算实际的时钟速率。

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