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A genechip analysis was performed using RNAs derived from embryos injected with squint mRNA, MZoep mutant embryos that are deficient in Nodal signaling, and wildtype embryos at the 30% epiboly stage Transcriptswith at least two-fold changes in expression level between wildtype and the other samples were identifyied In squint mRNA-injected embryos, 265 transcripts show anincreased expression level and 111 have a decreased expression level; in MZoep embryos, the expression of 1 495 transcripts increases while 550 transcripts express at a decreased level.

为鉴定受Nodal信号调控的基因，特别是那些转录因子基因，通过将来自squint过量表达、缺失Nodal信号的MZoep突变体或野生型30%外包期胚胎的RNA与Affymetrix斑马鱼寡核苷酸芯片杂交。

Part three: An experiment study on the repair of peripheral nerve injury by NT-3 gene modified neural stem cells objective To investigate the effective treatment of peripheral nerve injury with NT-3 gene modified neural stem cells, to explore the feasibility of the treatment by means of gene transfection Methods 54 SD rats were divided evenly into 3 groups. The sciatic nerve transected model were made and sutured end to end with epineurium. The neural stem cell without NT-3 gene or with NT-3 gene modified were injected into triceps muscle one time a week, saline injected as control group. The nerve trunks of stomas and triceps muscle were acted as specimen three weeks、six weeks and nine weeks after operation.

最后，为了探讨神经营养素-3基因修饰的神经干细胞对周围神经再生修复的影响，从转基因角度探讨治疗周围神经损伤的有效方法，我们选用54只SD大鼠随机分为3组，造成坐骨神经切断损伤模型，神经外膜端端对位缝合后，于小腿三头肌每周分别注射生理盐水、未被神经营养素-3基因修饰的神经干细胞、及神经营养素-3基因修饰的神经干细胞。

METHODS: Sixty Sprague-Dawley male rats were randomly divided into four groups(n = 15), four methods were designed on rats models of sciatic nerve compression. There were simple decompression as group A, internal neurolysis after decompression as group B, lemithason(0.5 mg/kg) injected in the epineurium after decompression as group C, and lemithason(0.5 mg/kg) injected around the epineurium after decompression and internal neurolysis as group D.

在大鼠坐骨神经卡压模型基础上，将60只SD成年雄性大鼠随机等分为四组。A组：仅去除卡压；B组：去除卡压后切开神经外膜；C组：去除卡压后神经外膜下周围注射利美达松(0.5mg/kg)；D组：去除卡压后切开神经外膜，然后在神经周围注射利美达松(0.5mg/kg)。

The main work can be summed up as follows: Firstly, we studied the thermal-field properties of VCSELs, and analyzed the influences of current spreading, material parameters and operating conditions on the temperature distributions. Secondly, we began with the electrode voltage and calculated the equipotential s distributions, compared the distributions of voltages and current densities in different depths of VCSELs, and then studied the influences of the oxide-confining region with different position or thickness, and the different sizes of the gain-guided aperture and emitting window on the distributions of the injected current density, carrier concentration and temperature in the active region. Thirdly, we realized the coupling of electricity, optical and thermal-fields, worked out the threshold voltage, calculated the distributions of the injected current density, carrier concentration and temperature under different offset voltages, and analyzed the impacts of temperature profile and carrier density on the refractive index, Fermi levels and optical-field. Finally, we gave the equipotential line distributions with considering N-DBR and double oxidized-confining regions, and analyzed theinfluences of N-DBR and double oxide-confining regions on the distributions of the current density, carrier concentration, temperature and optical-field.

具体工作可以概括如下：首先，研究了VCSEL的热场特性，分析了电流扩展，材料参数和工作条件对于温度分布的影响；其次，从电极电压入手，计算出激光器中的等势线分布，并对不同深度处的电压和电流分布进行比较，研究了高阻区的不同位置和不同厚度、限制层和出射窗口半径的大小对电流密度、载流子浓度和温度分布的影响；再次，实现了电、光、热耦合，求出了阈值电压，计算了不同偏置电压下的电流密度分布、载流子浓度分布和热场分布，分析了温度和载流子浓度变化对折射率、费米能级和光场的影响；最后，给出了考虑N-DBR和双氧化限制层时激光器中的等势线分布，分析了N-DBR和双氧化限制层对VCSEL电流密度、载流子浓度、温度和光场分布的影响。

Judkins catheter were placed in 5 dogs' left coronary arteries and home-made needle-catheter were injected in myocardium of another 5 dogs' left ventricular free walls.0.3 ml recombinant adenovirus vector containing 1×109 pfu was injected into either left coronary artery or myocardium or left ventricular free wall.

健康成年杂种狗10只，经股动脉插入冠造导管至左冠状动脉口(5只)和特制针型导管至左室心肌(5只)，注入0.3ml约含1×109 pfu腺病毒载体入心脏。

Then folic acid targeted lipisome mediated MYCN siRNA(3.5mg/kg/day) was injected daily into tumor mass,continuously for 3 days, while same volume of normal saline, with the same protocol was injected in mice with control. Three hours after last day injection, all the mice were sacrificed, tumor mass was collected for MYCN and Glyceraldehyde phosphate dehydrogenase mRNA expression detection by real time RT-PCR.

剩余SCID鼠，实验组局部瘤组织中注射叶酸包裹脂质体MYCN siRNA，剂量为3.5mg／kg，每天一次，连续三天，空白对照组相同部位、相同时间注射等量生理盐水，末次注射3小时后脱臼法处死动物，取肿瘤组织，提取总RNA。

Methods Twenty–four New Zealand white rabbits were divided into model group and control group randomly, of which the two groups which were made through the homemade impactive equipment by impacting the right acetabular cartilage of rabbit at 45cm with 0.400kg weight vertically and simulating the mechanism of impactive injury of human acetabular cartilage. Each group was 12.The model group was injected SH.The control group was injected NaCl solution. The observation of macrography, histology (stained by HE, safarnin O and toluidine blue), transmission electron microscope, scanning electron microscope and the method of TUNEL were made for the three groups that were killed postinjuriously at 4 day and 4 week.

方法新西兰大白兔24只，平均年龄6个月，体重2.5±0.5kg，模拟人髋臼软骨冲击伤机制，采用自制冲击装置，用质量为0.400kg的重锤，沿导向杆从高45cm处下滑垂直冲击兔右侧髋臼软骨，建立兔髋臼软骨冲击伤模型，随机分为模型组和对照组，每组12只。

In every group, rabbits were subdivided into experimental and control subgroups. 2 Rabbits were bullets injected followed by continuous injected with 13C labeled leucine, glucose, and lactic acid; 3 Blood were drewed before and 150, 160, 170, and 180 min after the initiation of isotopes injection for material analysis; 4 Exhale gas were collected every 5 min in the first 30 min followed by every 30 min there after for material analysis; 5 After centrifuge in low temp the supernatant of the blood samples were collected and went through axon and anon exchange column treatment; 6 Treated blood sample was used for 13C labeled leucine, glucose lactic acid examination through mass spectrograph; 7 The exhale gas was collected for 13CO2 exam through gas-phase mass spectrograph; 8 CO2 total production rate (V13CO2), body various substances production, oxidation speed, and substances metabolic percentage all can be calculated through equations provided by references below; 9 Data was processed through student t test.

分亮氨酸、葡萄糖和乳酸三组各取健康新西兰兔16只，每组再分为高代谢脓毒症组和对照组两部分，建模方法同第一部分；2）三组分别先静脉弹丸式推注再持续灌注13C标记的亮氨酸、葡萄糖和乳酸；3）灌注前抽取动脉血作为背景值，灌注达150，160，170和180min分别抽取动脉血2ml用于质谱分析；4）实验开始后前30min中每5min收集呼出气一次，随后每30min收集呼出气一次，用于气相质谱分析；5）血样经过低温离心取上清液，过阳离子及阴离子交换树脂，再经衍生化处理；6）处理过的血样进入气相色谱－质谱仪，测量其13C标记的亮氨酸、葡萄糖和乳酸丰度；7）实验兔的呼出气通过13CO2气相质谱分析仪测定其中的13CO2丰度（E13CO2）；8）利用文献提供的公式算出CO2总生成率（V13CO2）以及机体各物质的通量、氧化速率以及物质代谢百分比等；9）数据分析处理采用t检验。

Prussian blue staining revealed more than 98% of BM-MNCs were labeled by Feridex and BM-MNCs appeared to be unaffected by magnetically labeling. Three dogs injected Feridex- BM-MNCs were visualized at one week by MRI, but none of unlabeled BM-MNCs were seen. All lesions that were detected within one week could not be visualized over four week. The dot injected Feridex-cells which fixated by 4 % methanal also was seen at one week by MRI, but the signal disappeared at two week.

普鲁士蓝染色显示体外Feridex标记BM-MNCs成功率达98%以上，而且细胞活性不受影响；其中3只实验犬标记细胞移植区1周内可用1.5T磁共振仪3DSSFP序列在体扫描到T2低信号影像，4周时该低信号消失；同样条件下经甲醛固定的标记细胞在1周时也可检测到T2低信号，但2周时该低信号消失；未标记细胞移植区没有检测到低信号。

The authors research the algorithm for power system static voltage stability. Taking the modulus minimum eigenvalue of power flow Jacobian matrix as the criteria to weigh the weakness of power system, the active power injected into nodes equivalent is substituted by equivalent conductance to ground as well as the reactive power injected into nodes is substituted by equivalent susceptance to ground.

研究了电力系统静态电压稳定性算法，以潮流雅可比矩阵最小模特征值的大小作为衡量电网薄弱程度的标准，以等效对地电导代替节点注入有功功率，以等效对地电纳来代替节点注入无功功率。

In her eyes, because that is the doorway toher heart * the place where love resides.

女人的漂亮必須從她的眼睛中去看，因為那是她心靈的窗戶和愛居住的地方

I will send some postcards to you.

我会向你发送一些明信片。