查询词典 incubated

The researchers incubated the eggs, raised all the young together, then reintroduced the peafowl to the park--a few at a time in different batches. Three years later, 19 peacocks had established display territories in the park.

这些蛋孵化出来后，研究人员把幼孔雀混在一起喂养，然后分期分批放回公园。3年后，19只孔雀在公园里建立起了它们的演出区域。

Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合，而且结合是饱和性的、可被竞争的，符合配体-受体结合的典型特征，同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性，表明水稻细胞质膜上存在nsLTP的受体；利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜，再进行结合实验，结合活性几乎不受影响；而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失，表明其受体为没有经过糖基化修饰的蛋白质；利用交联剂BS~3交联配体一受体后，再进行SDS-PAGE分离和放射自显影，结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合，可能是其受体；细胞外碱化实验表明，nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应，却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应，表明nsLTP和Cryptogein结合细胞膜上相同的位点，保护了植物细胞免受Cryptogein导致的细胞程序性死亡，并诱导系统获得性抗性的产生；体外Pull-down实验表明，nsLTP144和水稻的OsCaM1具有相互作用，暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

METHODS: According to adherent + Thy1.1 antibody and complement-purification method, cranium was opened to expose olfactory bulb. Thereafter, two olfactory bulbs were obtained to remove cerebral pia mater, blood capillary, and peripheral tissues; additionally, olfactory nerve layer and olfactory bulb granular layer were sheared into 1-mm3 pieces for extract single-cell suspension. The cells were adjusted at the density of 1×107 /L and incubated with poly-l-lysine-coated culture bottle or culture plate in 5% CO2 incubator at 37 ℃. On the third day, cells were cultured with serum-free DMEM/F12 culture media.

在差速贴壁+Thy1.1抗体及补体纯化法的基础上，剪开大鼠颅骨，显露位于颅腔前方的嗅球，取出2只嗅球，在显微镜下去除嗅球表面的软脑膜和毛细血管及外周组织，保留富含嗅鞘细胞的嗅神经层和嗅球颗粒层，剪成1 mm3小块分离获取单细胞悬浮液，调整细胞密度至1×107 L-1，接种在用poly-l-lysine包被的培养瓶或培养板中，于37 ℃、体积分数为5%的CO2培养箱中培养，第3天用无血清DMEM/F12培养基换液培养。

Methods Synaptosomes was made from hippocampus and incubated with artificial cerebrospinal fluid. 30μmol/L propofol, 50μmol/L picrotoxin or both was added into aCSF. Intralipid was administered as solvent control and any drugs was applied in control. To observe Ca~(2+)-dependent release of glutamate and GABA, dihydrokainic acid and nipectic acid were added into aCSF.

制备大鼠海马突触体，随机分为5组：PTX组加入印防己毒素使其终浓度为50μmol/L，Pro30+PTX组加入终浓度为30μmol/L的丙泊酚和50μmol/L的PTX，Pro30组加入终浓度为30μmol/L的丙泊酚，溶剂对照组加入脂肪乳，空白对照组不加入上述药物。

Methods SH-SY5Y cells, a human neuroblastoma cell line, were incubated with different concentrations of fluoride for 48 hr and somle of them were treated with vitamin E precedently. The functional situation of cells was measured by MTT method; lipid peroxidation was detected by High-Performance Liquid Chromatography; phospholipid was separated by a Silica SepPak cartridge and neutral lipids by HPLC.

体外培养SH-SY5Y人脑神经母细胞瘤细胞，在培养液中加入不同浓度的氟化物或加入抗氧化剂，培养48h后用测定细胞MTT的方法来了解细胞的损伤程度，用高效液相色谱法分离和测定培养液中脂质过氧化物水平，用过柱和比色法测定细胞生物膜磷脂含量，用高效液相色谱法测定细胞生物膜辅酶Q和胆固醇含量。

In this study, observe the effect of UV protectant in Bacillus subtilis. Incubated in MIY liquid medium shaking at 30?

基於上述的限制，本研究以枯草杆菌作为研究对象，探讨其在不同浓度紫外线保护剂下之抗紫外线活性。

Intestinal microsomes of sea bream fed different lipid sources (fish, soyabean and rapeseed oils) at three different inclusion levels were isolated and incubated with l-[14C]glycerol-3-phosphate and [1-14C]palmitoyl CoA.

海鱼的肠微粒体用不同来源的油脂食物营养、用不同的含量，同时单独而且用碳14标记的3-磷酸甘油和棕榈酰辅酶A。

The aim of the present study was to study the effect of nicotine on ghrelin and the growth hormone secretagogue receptor expression, the effect of ghrelin on vascular cell adhesion molecule-1(VCAM-1) and interleukin-8 (IL-8) expression in nicotine incubated human umbilical vein endothelial cell,and the possible signaling transduction mechanisms associated with PKC, p38 MAPK and NF-κB.

本实验通过观察ghrelin对尼古丁所致HUVEC VCAM-1及IL-8表达变化过程中PKC、p38 MAPK及NF-κ3的活性变化，并应用PKC、p38 MAPK及NF-κB的特异性抑制剂chelerytherine chloride、SB203580、PDTC，探讨ghrelin阻断尼古丁引起内皮细胞功能紊乱致AS作用的信号转导机制。

The lenses of the control group were incubated without sodium selenite for the same length of time.

结果 与对照比较，实验组晶状体出现混浊的时间较早，混浊程度较重，并且呈浓度依赖性。

Methods: The DNA damage was measured after L-02 cell were incubated with 80μg/ml sodium fluoride, 1.73μg/ml selenium, 80μg/ml sodium fluoride and 1.73μg/ml selenium using Single Cell Electrophosis respectively.

采用80μg/ml氟化钠、1.73μg/ml亚硒酸钠以及80μg/ml氟化钠和1.73μg/ml亚硒酸钠对培养的L-02细胞进行染毒，24小时后用单细胞凝胶电泳技术检测各组L-02细胞DNA损伤情况。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。