查询词典 guinea pig

Methods:Single guinea pig ventricular myocytes were obtained by retrogradely perfusing with collagenase through the aorta.

用主动脉逆向灌流法酶解分离得到单个豚鼠心室肌细胞，在倒置显微镜下，选择质膜完整、横纹清晰的心室肌细胞，利用膜片钳技术经过封接、破膜、电容及串连电阻的补偿形成膜片钳全细胞记录状态。

Methods: By using microelectrode technique the action potentials on guinea pig papillary muscle AP(subscript PM and AP on sinuatrial node AP(subscript SN were induced and the effects of Zaoboniug granula on AP and AP were observed.

豚鼠20只分为早搏宁冲剂组(n=10)与盐酸普鲁卡因胺组(n=10)， 2组均采用累积浓度给药法给药，常规微电极技术引导豚鼠心室乳头肌快反应动作电位AP(下标 PM。另取豚鼠16只分为早搏宁冲剂组(n=10)与盐酸普鲁卡因胺组(n＝6)，同法给药、引导窦房结优势起搏细胞动作电位AP

Wong RK,Stewart M. Different firing patterns generated in dendrites and somata of CA1 pyramidal neurones in guinea-pig hippocampus.

结果发现，Mg2+浓度的提高虽可抑制正常的突触传递，但对LFS诱发LTD并无影响，A-PS的降低幅度仍显著大于S-EPSP。

Objective To perform an initial study on possible molecular regulatory mechanisms of TNF-α to the permeability of strial capillary endothelial cells in guinea pig cochlea.

目的 探讨肿瘤坏死因子α对豚鼠耳蜗血管纹微血管内皮细胞通透性的影响及可能的分子调控机理。

Methods Strial capillary endothelial cells in guinea pig cochlea was dissociated and cuhureed to establish a model for its permeability in vitro.,The animals were divided into TNF-α,TNF-α+L-arginine,TNF-α+NG-minomethyl-L-arginine and control groups.

体外分离培养豚鼠耳蜗血管纹微血管内皮细胞并建立内皮细胞通透性体外模型，观察TNF-α、TNF-α连同L-精氨酸(L-arginine，L-Arg)或L-单甲基精氨酸(NG-monomethyl-L-arginine，L-NMMA)对内皮细胞通透性的影响。

In the present study, we used styryl dye FM1-43 combined with stretch stimulation in the guinea pig esophagus to test whether IGLEs acted as the mechano-sensitive receptors of the vagal afferent nerves.

本研究应用活性依赖性荧光染料FM1-43结合牵拉刺激豚鼠食道显示激活的IGLEs结构，以期观察IGLEs是否对机械刺激敏感。

Methods Two-2D finite-element models were built based on the electron microscopic images of the organ of Corti from the guinea pig with two models of coupled and non-coupled between the tectorial membrane and stereocilia.

以豚鼠耳蜗底回Corti器的电镜图像为原型，建立盖膜与内毛细胞最长静纤毛接触和不接触的两种二维计算模型各一个，并作力学分析比较。

Results: The ranges of MIC of DNA-2E on trichophyton rubrum and beard-like trichophyton were 0.0625~0.125mg/mL and 0.03125~0.125mg/mL respectively; of which there was no significant difference between the two fungi. Both 2% and 4% DNA-2E had better effects on ringworm of guinea pig in feet and body infected by beard-like trichophyton, which showed significant difference compared to distilled water and no significant difference compared to 1% Zuguang powder. Both 4% and 6% DNA-2E had better Antipruritic effect, which showed no significant difference compared to 2% Zuguang Powder and significant difference compared to distilled water.

结果：癣清(DNA-2E)对红色毛癣菌、须状毛癣菌的最低抑菌浓度范围分别为0.0625~0.125mg/mL和0.03125~0.125mg/mL，两组比较差异无显著性；2%和4%癣清(DNA-2E)对豚鼠须状毛癣菌感染足癣、体癣有较好疗效，与1%足光粉比较差异无显著性，与蒸镏水组比较差异有高度显著性；4%和6%癣清(DNA-2E)止痒作用与1%足光粉相比差异无显著性，与蒸镏水组比较差异有高度显著性。

Blast underpressure ; auditory organ ; trauma model ; guinea pig

冲击波负压；听觉器官；创伤模型；豚鼠

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

If you are unfortunate enough to the lovelorn, please tell me, I will help you out, really, please contact me!

如果你不幸失恋了，请告诉我，我会帮助你摆脱困境，真的，请联系我啦！

China's plan to cut energy intensity by 20 percent and pollutant discharges by 10 percent between 2006 and 2010 is a case in point.

中国计划在2006年到2010间降低20%的能源强度和减少10%的主要污染物排放，就是一个这样的例子。