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We used Aeromonas hydrophila which we separated from the diseased Paralichthys olivaceus to made a inactivated whole cell bacterin .We injected this vaccine in the mice and the Paralichthys olivaceus .Both the mice and the Paralichthys olivaceus gained a good protection of 80% and 70%.The fish with dipping immersion gained a good protection of 85%,and the incidence of disease was 6%.We detected the antibody level in the antiserum by agglutination reaction and indirect ELISA which seemed to be immunogenic crucially.We anatomized the mice then we found that all the purtenance of the immunzied mice are healty.All of this proved that the vaccine is effective.

本实验将所分离到的嗜水气单胞菌用终浓度为0.2%~0.5%的甲醛对菌体进行灭活，制备了灭活疫苗，并优化灭活疫苗的灭活条件；将制备的灭活疫苗分别注射小鼠和牙鲆，均取得了较为理想的效果，其中小鼠的免疫保护率达到80%，牙鲆的免疫保护率达到70%；采用渗透压差法大规模浸泡免疫牙鲆也取得了很好的效果，浸泡免疫过的牙鲆发病率仅为6%，攻毒后免疫保护率为85%；通过应用间接ELISA方法检测受免动物的抗血清，并将牙鲆的抗血清注射新西兰白兔，从而获得兔抗鱼血清，更便于检测，结果都表明动物体内产生了较高的抗体水平；剖检攻毒后的动物，发现免疫过的动物内脏健康无异样，表明该疫苗安全有效，从而为防治牙鲆腹水症开辟了一条新途径，也为进一步制备其基因工程疫苗奠定了基础。

Russia took control of trade in the north, France gained exclusive trading rights in the South, and Germany gained concessions in Shantung.

俄罗斯得到了北方的租借权，法国得到了南方的租借权，德国租借了山东。

Aimed at the problem of choosing the initial value when the Newton method is used to compute the controlling unstable equilibrium point, a practical and rigorous solving scheme was presented: by identifying the controlling load bus of the given fault, and using the Thevenin equivalent circuit to represent the rest of the system at the state of the post disturbance stable equilibrium point, using the steady equivalent circuit to represent the induction motor in composite load, and then using the torque characteristics of induction motor, a point near the CUEP is gained to be the initial value. The second order normal forms was used to approximate the stable manifold of CUEP, and the local approximating boundary of the region of attraction of the post disturbance stable equilibrium point was gained. Then just by simulating the state of the system at the fault clearing time, the transient voltage stability of the system could be determined.

针对采用牛顿法求取故障后系统主导不稳定平衡点(controlling unstable equilibrium point，CUEP)存在的初值选取难题，提出一种实用但不失严谨的解决方案：通过识别给定故障的主导负荷母线，对主导负荷母线以外系统由故障后稳定平衡点处的状态进行戴维南等值，对负荷中感应电动机部分采用其稳态等值电路，再由感应电动机的转矩特性求得CUEP附近的一个点作为近似的CUEP，以此为迭代初值可靠求得CUEP；采用二阶正规型来近似CUEP的稳定流形的方法求得近似的局部吸引域边界；由仿真得到故障清除时刻系统的状态并根据该状态是否位于吸引域内判断系统的暂态电压稳定性。

Based on the example of turbine reverse engineering , the general procedure that can be used to translate the physical object into CAD datum by Surfacer software is determined in this paper1 By going deep into the vector of point-cloud , it's parametrization can be gained by adopting the precept of accumulating chordal parametrization1 According to the principle of const ructing curve using the least square method , the high quality-curve can be gained by modifying curve's parameter1 This paper also empha2sizes on some methods about processing point-cloud , const ructing curve and const ructing surface1 And it can provide several methods to const ruct high quality2CAD model for mould machining1

此栏目为免费论文，可自行复制获取，如需要其他完整论文，可点击论文范文查找摘要本文就涡轮反求造型的实例，采用Surfacer 软件规划出由实物转化为工程CAD 资料的一般流程。在深入分析点云数据的矢量方向基础上，采用积累弦长参数化方案实现点云的参数化；根据最小二乘法曲线拟合原理，修改曲线参数获得高品质曲线；并重点论述了点云处理、曲线生成、建构曲面的方法，为模具加工提供了建构高品质CAD 模型的有效途径。

I was very happy when I gained honor myself, but I was more joyful and athrill when my class gained one.

但是我深知没有成功的个人，只有成功的集体。个人的成绩让我高兴，但集体荣誉更让我愉悦，激动！

Isolations of the s.aureus insoluble peptidoglycan:(1) the s.aureus insoluble peptidoglycan was gained by cold, hot and hypersound affection repetat of s.aureus(ATCC25923);(2) the s.aureus insoluble peptidoglycan was gained by 10% trichloracetic acid, 5% trichloracetic acid,NH4HCO3 buffer with trypsin digestion of s.aureus(ATCC25923) in order. 2. Isolations of the s.aureus soluble peptidoglycan and quality control:(1) the s.aureus soluble peptidoglycan was released by short dose benzylpenicillin sodium and purified by sephadex G-100.(2) The quality control in the process of isolation: the soluble peptidoglycan was detected by SLP reagent kit; the LPS was detected by kinetic turbidimetric limulus test;and the protein was detected by improved LOWRY reagent kit.

金黄色葡萄球菌可溶性肽聚糖的分离提取及其质量控制(1)采用青霉素钠诱导法和葡聚糖G-100(Sephadex G-100)凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖；(2)金黄色葡萄球菌可溶性肽聚糖提取过程中的质量控制：采用SLP试剂盒对提取物定性检测，应用动态浊度法鲎试验测定提取物中内毒素含量，采用改良LOWRY试剂检测提取物中蛋白的含量；(3)采用青霉素诱导和Sephadex G-100凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖的条件：青霉素使用量、加入青霉素后孵育时间和菌液浓度等优化选择。

To overpass improvement of experimentative method, experimentative integrality gained advance. The exercitation of integrativn ability of students gained aggrandizement.

经过此番实验方法改良，使实验的整体性得到提高，对学生的综合能力训练得到强化。

For obtaining the numeral solutions of round elastic plates in axisymmetrical deformation with varying-thickness in nonlinear deformation in the unsteady electromagnetic field and mechanical field,by establishing the equations of motion,the equations of distortion,the equations of Lorentz force,the equations of electronic dynamic mechenical,and adopting iterative method,we gained the nonlinear magneto-elastic calculation method and outcome of current-carrying shell,and gained the influencing degree toward mechanical parameter of varying thickness round ferreous plates in the electromagnetic field and mechanical field.

为了获得内边界固定的环形导电薄板在均布力与横向磁场作用下的变形问题的数值解，通过建立运动方程、变形方程、电动力学方程和洛仑兹力方程，运用迭代法得出了载流板壳的非线性磁弹性的计算方法和结果，以及机械场、电磁场对铁质变厚度圆板各力学参量的影响程度。

The basic principle is that basic geometry shape of openings in the photo is revised; the image can't be distortion by use of projection reconstruction; the corrected image is exerted by open operation and close operation of gray-image; the shape of openings is gained lastly. The perimeter borderline of openings shape is extracted. The quantity of overbreak as well as underbreak is gained by comparing to extracted perimeter and designed perimeter. The same method is applied to other opening sections. Evaluation of overbreak as well as underbreak in the openings can be easily solved in the way of quantitative.

其基本原理是：对野外摄取的洞室的基本剖面形状进行几何校正，然后应用图像学中的投影重建方法使图像不失真，对修正后的图像实行灰度图像的开操作和闭操作运算，得到洞室的基本形状，然后用图像学中的边界提取技术提取洞室的周边界，与洞室的设计形状对比分析，得到洞室在该断面上的超欠挖方量，然后对其它断面采用同样的技术就可评价某一段或整个洞室的超欠挖数量，从定量的角度解决了工程中的超欠挖评价问题。

In the time of the Buddha, many who gained perfection through insight meditation also gained supernormal powers.

但取得这些能力并非谛观的目标。

Who? I never heard of him, Paul said, before asking teammate James Posey if he had heard of him.

赛后，科比说，他一直都是一名非常出色的射手，今天他打得很棒。

When I joined the company, I rotated around the different sections.

我加入这个公司时，轮换过几个不同的部门。