查询词典 fusion welding

This is the first study to our knowledge to use standardized outcome measures to assess clinical outcome in patients treated with posterior fusion for pseudarthrosis after anterior cerical discectomy and fusion.

据我们所知，这是首次使用标准结果评估后路融合治疗前路椎体切除和融合术后有症状的假关节患者的临床结果。

［Objective］To compare the clinical results of different bone graft in the posterior route spinal fusion of adolescent idiopathic scoliosis.［Method］A retrospective study was made in 71 case of adolescent idiopathic scoliosis,who were operated on with posterior instrumentation of screw-rod fixation system from 2000 to 2005.All of the patients were divided into three groups,autologous bone graft in 21 cases,allogous bone graft in 23 cases, autologous bone mixed allogous bone graft in 27 cases. The fusion rate, the Cobb's angle loss rate of incidence and pseudoarthrosis were compared among the three groups at 3,9,15,36 months follow-up.

［目的］通过不同植骨在青少年特发性脊柱侧凸后路矫形植骨融合术中的应用比较，探讨其临床应用效果［方法］回顾性分析2000年～2005年行后路钉-棒系统矫形手术的71例青少年特发性脊柱侧凸患者，其中自体骨移植21例，同异体骨移植23例，自体骨混合同种异体骨移植27例，比较随访第3、9、15、36 月的融合率、Cobb's角丢失率及假关节发生率。

BACKGROUND: Metal vertebral fusion cage can induce some disadvantages and pseudoarticulation formation such as stress dodge, fusion cage subsidence, bone resorption, prolapse and bone collapse.

摘要背景：金属椎间融合器存在应力遮挡、融合器下沉、骨吸收、脱出、骨塌陷所带来的并发症和假关节形成等，影响融合效果。

Objective To construct an expression vector for fusion gene of heat-liable enterotoxin B subunit and major outer membrane protein of Chlamydia psittaci and express the fusion gene in prokaryotic cells.

目的构建含大肠杆菌热不稳定肠毒素B亚单位和鹦鹉热衣原体主要外膜蛋白融合基因的表达载体，并在原核细胞中表达融合蛋白。

Otherwise considered the axis and atlas fusion surgery or the pulvinar neck fusion technical according to extent of damage and type formulating surgery plan .Conlusions: X-ray inspection was routine method of the cervical part injure .

骨性结构损伤和韧带断裂或脱位者，考虑行寰枢椎融合手术或者枕颈融合术，具体根据损伤程度和类型制定手术方案。

METHODS:The gene of human ET1 was synthesized according to the preferential codons of E. coli, cloned into the EcoRI and SalI sites of vector pThioHisA. The recombinant plasmid pThioHisA-ET1 was constructed , sequenced and transformed into E.coli TOP10. Induced and expressed fusion protein were identified and analysed by 12% SDS-PAGE and densitometry analyses. After the elution, denature and renature, the fusion protein Thioredoxin-ET1 was obtained by ProBondTM chromatogragraphy. The purity of Thioredoxin-ET1 was detected by HPLC. Inoculate Thioredoxin-ET1 once per mouse every 2 weeks in 25, 50 and 100μg separately on 3 groups for 4 times. 10 days after last inoculation, we obtained venipuncture blood.

根据人ET1的多肽序列合成ET1基因，将其插入到pThioHisA的EcoRI和 SalI位点，重组质粒pThioHisA-ET1进行酶切鉴定及序列测定验证后转化TOP10，IPTG诱导的重组菌经SDS-PAGE检测融合蛋白Thioredoxin-ET1的表达量；表达的融合蛋白用ProBond亲合层析纯化并经HPLC鉴测其纯度；每只小鼠按25、50、100ug/次剂量的Thioredoxin-ET1每两周免疫一次，共4次，最后一次免疫10d后制备抗血清，经Western blot和ELISA检测证明Thioredoxin-ET1融合蛋白具有ET1免疫反应原性。

A complete bovine 18ku-bFGF gene was cloned by nested-PCR and subcloned into expression vector pET-28a.The recombinant plasmid pET-28a-bFGF was transformed into Escherichia coli BL21.At 25℃,recombinant protein was induced by 0.5mmol/L IPTG for 5 hours.Supernatant of cell lysate was purified using Ni-NTA method and the products was submitted to detect the bioactivity by Western-blotting,which indicated that the fusion protein contained recombinant bovine bFGF.Results showed that recombinant bovine bFGF was produced and solubly existed in the supernatant.NIH-3T3 fibroblasts were used to detect the bioactivity of the fusion protein.

采用巢式PCR方法克隆了牛18ku-bFGF基因完整的编码序列，并构建了原核表达载体pET-28a-bFGF，将其转化大肠杆菌BL21，在25℃低温条件下，用0.5mmol/L IPTG诱导表达5h，用Ni-NTA亲和纯化细胞裂解上清液，经Western-blotting检测，结果显示，在特定的诱导条件下，重组牛bFGF基因在大肠杆菌中获得了表达，并且主要以可溶性状态存在于细胞中。

Fusion of like parts, as the fusion of staminal filaments into a tube.

合生的 Connate 相似部分的结合，如雄蕊的花丝合生成筒。

By the affinity chromatography,fusion protein is purificated. The antiserum of chicken and rabbit were prepared with the fusion protein and Ghrelin-KLH.With active immunity and passive immunity,confirmed that Ghrelin of chicken have no obviously impact on ultimum metabolite,plasma Insulin,glucagons, T_3 and T_4.It entrances plasma GH,but inhibit food intake and increase of fat and body weight.These conclusion are obviously different from the mammal.

以纯化的融合蛋白和人工合成的Ghrelin-KLH为抗原，制备了鸡、兔抗血清：通过主动免疫、被动免疫中和实验证实禽类Ghrelin对鸡血浆中代谢产物、胰岛素、胰高血糖素、甲状腺素浓度没有明显的影响，对GH的分泌有促进作用，但与哺乳动物中不同，Ghrelin抑制鸡的脂重、体重的增加，抑制鸡的摄食。

Two arithmetics that are crisp fusion arithmetic and uncrisp fusion arithmetic will be discussed in the paper. The basic principle and application scope will be introduced in the dissertation.

文中介绍了两种情况下进行知识提取的基本原理以及它们在分布式检测系统和证券分析系统中的应用方法。

It was just a normal day of school

那只是上学的普通一天

Which is a very good reference in this issue.

这是一个在这个问题上很好的参考。