查询词典 fusion welding

Specifically, itcontains 8 chapters.In chapter 1, the formation, structures, properties and the futureprospect of liposome were thoroughly reviewed;In chapter 2, the stibility and permeability of phopholipid -eleostericacid liposome were studied together with the effect of polymerizationof eleostearic acid. This membrane system was very sensitive to 〓,the effect of 〓 was clarified to increase the aggregation/fusion ofliposomes and made the permeability of mixed liposomes much higher;In chapter 3, two polymerizable conjugated diyne bolaamphiphiles were synthesized. They could form very stable mixed liposome, andthe diyne could be polymerized by UV light in bilayer liposomes, as aresult, the stability of mixed liposome against solvent or surfactantafter polymerization were enhanced. In chapter 4, two kinds of amphiphilic amino acids were synthesized andstable liposomes were formed therefrom After the condensationpolymerization of amino acid in bilayer liposomes, stable polypeptide liposomes were obtained, which had lower phase transition temperatureand higher permeability.In chapter 5, four kinds of glycolipids were synthesized and theiraggregation behavior in water was comparied. When incorporated intophospholipid bilayer membranes, they could increase the phase transitiontemperatures and inhibit the aggregation and fusion of mixedliposomesat lower temperature.In chapter 6 and 7, three kinds of steroidal bolaamphiphiles withdifferent chain lengths were synthesized. Incorporation of steroidalmoiety to the center of lipid bilayer membrane obviously increased themobility of lipid membrane and shifted Tc to lower temperature side incomparasion with cholesterol. The bolaamphiphile which was shorter thanthe hosted lipid bilayer membrane thickness influenced the lipid packingmore obviously.

全文共分8章：第一章对脂质体的形成、结构、性质及展望进行了较为详细的文献综述；第二章研究了磷脂-桐酸脂质体的稳定性，通透能力及桐酸的聚合对这些性质的影响；磷脂-桐酸混合脂质体为一类对〓灵敏的脂质体，〓的作用首先是使脂质体集聚然后使脂质体融合，并加速内包荧光物的释放；第三章通过合成两种可聚合共轭双炔双极性双亲分子DDCA，DDOL，研究了共双炔分子在双分子层脂质体膜上的聚合及对脂质体性质的影响，聚合可以提高脂质体相对于溶剂及表面活性剂的稳定性；第四章合成了两类氨基酸为极性基团的双亲分子，它们均可以在超声下形成稳定的脂质体结构；氨基酸基团可以在脂质体上进行缩聚反应，若聚合后脂质体表面仍有足够的亲水能力，则可得到稳定的多肽型脂质体；聚合后脂质体的相变温度降低，通透能力增加；第五章合成了四种亲水基团为单糖基的双亲分子GL-l，GL-2，GL-3， GL-4，研究了它们在水中的分散情况、集合体形态与分子结构的关系；在DMPC双分子层膜中加入糖脂分子可以使脂质体的相变温度提高，阻止脂质体在低温放置时的集聚与融合；第六章-第七章合成了三种不同碳链长度的双极性含胆甾环双亲分子 CL-1，CL-2，CL-3；它们可以象胆固醇一样与磷脂混合形成稳定脂质体，胆甾环基团位于脂质体双分子层膜的中间；与胆固醇的作用相反，它们可以增加磷脂双分子层膜的流动性，降低混合脂质体的相变温度；三种分子的作用与其碳链长度和磷脂双分子层膜的厚度有关，比膜厚度短的分子影响最大。

Application of multi-modality medical image fusion technology in the fusion of MR and PET images can improve the accuracy of the localization of epileptogenic foci and provide more accurate diagnostic information for the surgical therapies and three-dimensional orientation radioative treatments.

利用多模医学图像融合技术将MR与PET的影像融合能明显地提高癫痫病灶定位诊断的准确率，为癫痫外科手术治疗和立体定向放射外科治疗提供更准确的信息。

Application of multi-modality medical image fusion technology in the fusion of MR and PET images can improve the accuracy of the localization of epileptogenic foci and provide more accurate diagnostic information for the surgical therapies and three-dimensional orientation radioative treatments.

详细摘要癫痫灶的准确定位是决定癫痫外科疗效的关键步骤。利用多模医学图像融合技术将MR与PET的影像融合能明显地提高癫痫病灶定位诊断的准确率，为癫痫外科手术治疗和立体定向放射外科治疗提供更准确的信息。

The fusion expression vector and the non-fusion expression vector were constructed by the open reading frame of annfaf expressed specifically in strawberry Fragaria ananassa Duch.

将草莓？穴Fragaria ananassa Duch。？雪果实中特异表达的膜联蛋白基因annfaf的cDNA连接重组到pET-30a质粒中，构建了融合和非融合蛋白表达载体。annfaf 基因转化大肠杆菌E。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

Then the fusion protein was primary purified by affinity chromatography. The GST tail of the fusion protein was cleaved by Thrombin. Nucleotide sequence analysis showed that the gdh gene of S.suis possesses the highly conserved motifs typical of family I hexameric GDHs.

猪链球菌2型谷氨酸脱氢酶编码基因克隆、序列分析及原核表达根据已发表的猪链球菌2型的gdh基因设计引物，扩增海安病人分离株Habb中的目的基因，并进行序列测定和分析；构建重组表达载体pGEX4T-2-gdh，在大肠杆菌中表达，并纯化重组蛋白。

At last, ILIF in segment L3-4 and L4-5 is feasible. 2. There are enough operation space for ILIF in L3-4 and L4-5 with the root being retracted gently. And it is feasible to insert a couple transpedicle screws homolaterally. The intertransverse area was too narrow to perform ILIF on L5-S1.3. ILIF provide greater biomechanical stability than TLIF. while there was no significantly different stability between ILIF with homolateral or bilateral pedicle screw fixation. Adequate postoperative stability can be achieved by intertransverse lumbar interbody fusion with homolateral pedicle screw fixation.4. ILIF with transpedicle screws fixation homolaterally was a safe, convenient and effective operation, and less intraspinal complications were occurred of ILIF than TLIF.5. The fusion condition was satisfaction in ILIF group, which was better than TLIF group.

横突间入路腰椎椎体间融合术在L3-4、L4-5节段具有可行性。2、在L3-4、L4-5节段可提供足够的空间行ILIF手术，并可通过同一切口附加椎弓根钉固定，操作安全、简便。L5-S1节段横突间区域较小，操作困难，不适合行ILIF手术。3、ILIF手术术后生物力学稳定性有所下降但优于TLIF手术；ILIF附加同侧椎弓根钉固定与附加双侧椎弓根钉固定生物力学稳定性相当，使用ILIF术式附加侧同椎弓根螺钉固定，可提供较好的即刻稳定性。4、ILIF手术及附加同侧椎弓根钉固定是一种安全、简便、有效的手术方式，在减少椎管内并发症方面具有优势。5、ILIF手术可获得较好的融合率，其融合情况从组织学观察稍好于TLIF手术。

The protoplast fusion between hyperparasitic Streptomyces strain F46 and Actinomyces strain SC11 was studied in order to improve the effect of wild actinomyces strains to control plant diseases, solve the problems in application of the biocontrol strains, explore the method of protoplast fusion and get the recombinants with some excellent characteristics of both parents.

为了提高野生生防放线菌株的防病效果，克服以往生防菌应用方面存在的诸多弊端，探索简单易行的人工改良生防放线菌的途径，我们选用重寄生链霉菌F46和放线菌SC11作为亲本，进行了双亲原生质体融合研究，以期得到整合双亲多种优良性状的工程菌株。

Inertially confined fusion, especially the research on ultra short intense laser pulses and materials, used in a new technology of laser fusion called "fast ignitor", gain a growing attention.

惯性约束核聚变，特别是新型&快点火&激光聚变所涉及的超短超强激光脉冲与物质相互作用的研究，越来越受到人们的关注。

Pallidum-antibody with indirected-ELISA based on the fusion protein. Zealand rabbits were immunized with the fusion protein, antibodies to anti-Gpd in sera were detected with indirected ELISA.

同时用纯化的Gpd重组蛋白免疫新西兰兔，间接ELISA方法检测免疫兔血清中Gpd多克隆抗体的效价，对Gpd重组蛋白的免疫原性进行分析。

It was just a normal day of school

那只是上学的普通一天

Which is a very good reference in this issue.

这是一个在这个问题上很好的参考。