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The study on the phylogenetic relations among these species is very useful for the study on genome and marker-assisted breeding in Brassica species. The karyotypic analysis is one of the most important methods in analysing the phylogenetic relation of species with closer relations. The protocol for inducing high frequency metaphase was established by pretreatment with low temperature and 8hydroxyquinoline, and then the preparation of chromosome and karyotypes analysis of six economical important Brassica species were carried out. According to the karyotypic analysis, it was shown that in the genomes of amphidiploid species, there are genomical components from didiploid species. Our result showed that B. campetris, B. juncea and B. napus belonged to class 2B, while B.

以二倍体B.nigra（B，n=8）基因组为探针，用B.campestris（A，n=10）或B.oleracea（C，n=9）的基因组进行封阻，分别与B.juncea（AB，n=18）和B.carinata（BC，n=17）进行GISH杂交发现，B.juncea和B.carinata基因组中仅有来自于B.nigra的8对染色体表现杂交信号，而其它染色体杂交信号较弱；以B.campestris或B.oleracea基因组为杂交探针与B.napus（AC，n=19）进行GISH杂交发现，所有19对染色体均出现杂交信号，根据杂交结果难以正确判断B.napus基因组中的哪些染色体是来自于A基因组，哪些是来自于C基因组；以rRNA基因（18S-5.8S-26S）为探针的FISH杂交结果表明，B.campestris基因组中rRNA基因位点为6对；B.oleracea为3对；B.nigra为2对；B.carinata为4对；B.juncea为6对；B.napus为6对。

He color which is more deep of Eye Shadow is the focus of the entire part, it will be able to make with light-colored eye shadow with the dark on the first slightly grazed in the back of the hand, so that a more homogeneous makeup on, and then applied to the eye's tail part from eye outside to the orbital showing out the eye smudge homogeneous, not to reveal the depth of the apparent boundary of the two colors can be so profound a sense of the eye.

ips3：深色系重点画法颜色比较深的眼影是整个眼妆的重点部份，将可以与浅色系作搭配的深色眼影先於手背上稍微擦过，让上妆更加均匀，然后将其涂抹於眼尾处，并由外眼角向眼窝呈放射状均匀晕染，切勿显露出深浅两色的明显界线，就可让眼睛呈现深邃感。

One people huddles up on bed , has no way to fall asleep, be thinking of the absurd problem in the brain, dark cools down just like a gigantic cobweb is good with my parcel apud , profound cotton-wadded quilt lining , my feeling, from fishing out mobile telephone under the pillow, but find that do not know should whom to fight to, thereupon, unwilling hold on to self knee lightly , cannot only be capable to do the cold bar then like one child holds self tightly one point in squeezing time of pillow in , in headman indent quilt,...

一个人蜷缩在床上，无法入睡，脑子里想着莫名其妙的问题，黑暗犹如一张巨大的蛛网把你包裹在内，厚厚的棉被里，我感觉好冷，从枕头下掏出手机，却发现我不知道该打给谁，于是，无奈的又塞回枕头中，把头缩进被子里，轻轻的抱住自己的膝盖，像一个孩子把自己抱紧一点，那么，就不会冷了吧

Based on the cloning FeSOD gene of the HZNH, the recombinant prokaryotic expression vector, PQESOa/FeSOD, was constructed and digested with restriction endonuclease BamH I and Pst I to check its construction. The PQE30a/FeSOD was then transformed into E.coli Ml5 and induced with IPTG. The high expression in vitro was obtained and analyzed on SDS-PAGE gel. The*results showed that the target proteins held a 37% portion in whole bacterial proteins and consisted of two parts, the soluble proteins and inclusion bodies. The soluble proteins in the aqueous layer, checked by means of activities of FeSOD enzymes and analyzed by means of activities of isozymogram from PAGE, demonstrated the induced expression proteins had the active nature of FeSOD enzymes.

以克隆的特异种质烟草HZNH的FeSOD基因为基础，构建了原核表达载体PQE30a／FeSOD，经限制性内切酶BamH Ⅰ、Pst Ⅰ双酶切鉴定后，再转化入大肠杆菌M15中，通过IPTG诱导，得到高效体外表达，经SDS-聚丙烯酰胺凝胶电泳检测，表达的目的蛋白占总菌体蛋白的37％，可溶性和包涵体两种形式均有存在，上清中的可溶性蛋白经FeSOD酶活测定和同工酶活性谱带分析，表明诱导表达的上清中的目的蛋白为有活性的FeSOD酶。

For the status of the fishing technology of deep and ultra-deep slim hole was started later, the lack means of dealing with accidents and little fishing experience on the oil field, from the actual conditions of the Tarim oil field and in research on the basis of information at home and abroad, this article analyzed the technical difficulties of fishing in the deep and ultra-deep Slim hole and the importance of preparations before fishing, and then summarized the powdered magnesium technology, sets milling fish-neck technology , Venturi joints with milling technology and fishing rope-type fish with spiral bailer grab technology.

针对深井超深井小井眼打捞工艺技术起步较晚，事故处理手段单一及现场打捞经验不足的现状，本文从塔里木油田实际情况出发，在调研国内外资料的基础上，分析了深井超深井小井眼打捞的技术难点及打捞前期准备的重要性；归纳总结出了适合塔里木地区深井超深井小井眼事故处理的镁粉切割工艺技术、套铣鱼颈工艺技术、文丘里接头配合磨铣工艺技术及螺旋捞钩打捞绳类落物工艺技术。

Then Hualin Securities Research Institute, deputy director of the Liu's survey article," In 2003 the state's tax department to carry out tax reform, the banking business tax rate From 5% to 3%, it has never fully implemented because the bank is a local business tax the bulk of the tax on local revenue a greater impact on bank profits at the same time it also has great influence, such as sales tax in 2004, accounting for listed banks set aside Bizen an average of 20% of the profits, net profit of more than 30%, the domestic banks to lower the tax burden has been higher voice."

然后华林证券研究所，刘的调查文章，在2003年"副主任国家税收部门进行税制改革，银行业务由5％税率3％，但从来没有充分执行，因为银行是本地的营业税对地方收入税的大部分银行的利润就更大，同时它也有很大的影响，如2004年的销售税，对上市会计的影响备前银行预留了20％的利润，平均超过30％的净利润，在国内银行降低税负却远的声音。"

Du Bois went on to attend Fisk University in Nashville, Tennessee, for two years and then went on to earn his B.A. and Ph.D. from Harvard University.

杜博斯在田纳西州那什维尔的费斯克大学念了两年大学，然后又在哈佛大学取得他的学士及博士学位。

On formal dinning occasion it's good manner to take some butter from the butter dish with your bread knife and put it on your side plate. Then butter the pieces of the roll use this butter.

在正式的用餐场合有一条好的礼仪就是，当你在吃面包涂黄油的时候，最好先用你的面包刀把黄油盘里的黄油挖出来点放到你的面包碟里，然后再用这些黄油涂抹你的面包。

Using the aforementioned experimental methods,the author has performedcareful study on the thermoanalytical characteristic of nanocrystalline brookite-basedTiO2,and found that on the DTA curve there exists a small irreversible endothermicpeak,which is related to the dramatic structural phasetransformation from brookite to anatase then to rutile.

利用上述实验手段，作者首次对板钛矿基(brookite-based)纳米TiO2的热分析特性进行了较系统、深入的研究，发现在DTA曲线上存在一较小的不可逆、无失重吸热峰，对应于从板钛矿经锐钛矿到金红石的急剧转变。

The main object of this research is to investigate synthesis of Cu23Cl Cu and CuO nanoparticles through electrospinning and heat-treated method The research is mainly focused on preparative parameter and the change of heat-treated temperature to form the nanoparticles inlaid in silicon fibers the shape and color change are dicussed First of all to prepare the Polyvinyl butyral and silica dioxide complex nanofiber by electrospinning and then to investigate different heat-treated temperature and reactive time to being an influence on the products The results detected by SEM XRD TEM EDS FTIR combined sol-gel process and electrospinning can prepare Polyvinyl butyral and silica complex nanofiber The experimental result is found when heat-treated temperature is 100~4750C it can produce Cu23Cl nanoparticles; Above 4750C~ 4900C it can produce Cu nanoparticles; Above 450~7000C it can produce CuO nanoparticles And the viscosity is lain between 20~40cp and the sol-gel process time is 3hr it can produce the thinner fibers The average diameter of the fibers are 107 88±21 01nm;Due to the nanoparticles inlaid in the silica fibers the thinner fibers can be inlaid the smaller nanoparticles so this is the result that the experiment is expected To calcine the complex fibers is to produce surface silica fibers contain Cu23Cl Cu and CuO nanoparticles due to surface plasma resonance it make the color of the fibers become yellowish green from light white green turn into the red nuclear finally As the experimental result to utilize sol-gel process combine electrospinning can produce porous silica fibers contain Cu23Cl Cu and CuO nanoparticles

本研究旨在探讨「利用放电纺丝和热处理法来合成Cu23Cl、Cu和CuO奈米粒」之研究，实验著重在制备参数与热处理温度变化对所形成的奈米粒镶嵌在二氧化矽纤维中的形态与颜色变化探讨。首先，利用放电纺丝法制备出聚乙烯醇缩丁醛及二氧化矽之奈米纤维，比较不同的热处理温度与反应时间的改变对产物生成产生影响，进而研究不同热处理温度和时间对生成奈米粒的影响。产物经由SEM、XRD、TEM、EDS和FTIR等仪器分析结果显示，结合溶胶-凝胶法(sol-gel process)和放电纺丝法可产生聚乙烯醇缩丁醛及二氧化矽复合奈米纤维。实验结果发现，若热处理温度在100~4750C下可得到Cu23Cl奈米粒，475~4900C 可得Cu奈米粒，450~7000C以上可得 CuO奈米粒。而黏度介於20~40cp间和溶胶-凝胶时间为3hr时，可产生直径比较细的纤维，纤维直径为107 88±21 01nm；且由於奈米金属颗粒镶嵌在二氧化矽纤维中，直径比较细的纤维，可以得到比较小的奈米金属颗粒，这与实验预期相符。而锻烧此复合物产生多孔的二氧化矽纤维并包含Cu23Cl、Cu和CuO奈米微粒时，由於表面电浆子共震关系，而使纤维颜色由淡白黄绿色变成黄绿色，再变红褐色。由实验结果得知，利用溶胶-凝胶法(sol-gel process)结合放电纺丝法和不同热处理温度，可产生Cu23Cl、Cu和CuO奈米粒镶嵌在二氧化矽纤维。

Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

万寿菊对土壤要求不严，以肥沃。排水良好的砂质壤土为好。

He unstepped the mast and furled the sail and tied it.

他拔下桅杆，把帆卷起，系住。