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Real time RT-PCR analysis showed that in compatible interaction between Pst and wheat, PsCdc2 was up-regulated at early stage of infection. The maximum induction occurred at 12 hpi, at which transcripts were 1.62 fold over that in urediniospore. From 24 to 268 hpi, the accumulation of transcripts decreased steadily.

实时荧光定量RT-PCR结果表明，在亲和组合中，该基因在条锈菌接种小麦的前期上调表达，其中接种后12 h时表达量最高，约为夏孢子中表达量的1.62倍，接种后24-268 h，基因表达基本呈下调趋势，其中96 h基因表达量最低，仅为夏孢子时期的0.07倍。

Sales in our company have increased up to four-fold in the last five years.

例10 过去五年中，我企业的销售额已增长至四倍。

Sales in our company have increased up to four-fold in the last five years.

例10 过去五年中，我公司的营业额已增长至四倍。

Sales in our company have increased up to four-fold in the last five years.

The 例 10 过去五年中，我公司的销售额已增长至四倍。

Sales in our company have increased up to four-fold in the last five years.

例10 过去五年中，我公司的销售额已增长至四倍。本文来好考试

Sales in our company have increased up to four-fold in the last five years.

例10 过去五年中，我公司的**额已增长至四倍。

The dilution does not appear to have hurt Ford's share price, which is up four-fold since late February.

股权的流失并没有损害到福特的股价，反而在今年二月底以来上涨了16倍。

Over that time, the company says its own retail revenues were up three-fold.

在那段时间里，公司说其零售销售总额上升了3倍。

Over that time, the company says its own retail revenues were up three-fold.

还称在这期间，公司自身的零售业务收入翻了三倍。

What's more, the entrepreneur described with pride that once he had bought up all of his competition, he was able to raise his prices three-fold.

此外，该主管骄傲地说，一旦收购了所有的竞争对手，他就可以将价格涨到原来的三倍。

The second is to cut a mere 100 calories from your diet , which is the equivalent of a tablespoon of butter.

第二个就是从你的饮食中减少一百卡路里，只是相当于一大汤匙的黄油。

This is where the Rorschach test comes in.

现在，罗氏测试就出现了。