查询词典 fermentation method

A screening and cultivation method for Bacillus thuringiensis of high-yield high temperature resisting protease includes the following steps: high-yield high temperature resisting protease Bacillus thuringiensis Bt140 is obtained through adopting casein substrate agar to identify flat primary screen and fermenting enzyme activity measuring secondary screening; Plackett-Burman design and response surface analytical method are adopted to obtain optimal fermentation zymogenic formula and fermentation condition for Bt140 quickly; finally, shaking fermentation level reaches to 918.56U/mL.

一种高产耐高温蛋白酶的苏云金芽孢杆菌筛选及培养方法，采用酪蛋白底物琼脂鉴定平板初筛与发酵酶活测定复筛的方法获得高产耐高温蛋白酶苏云金芽孢杆菌Bt140；采用Plackett-Burman设计与响应面分析法快速获得了Bt140最佳的发酵产酶配方与发酵条件，最终摇瓶发酵水平可达918.56U/mL。

NJWGY3665 bacterial strain is inoculated in a glucose nutrient culture medium to carry out the slant culture, the culture temperature is 28 to 37 DEG C and the culture time is 2 to 6 days;(2) seed culture: a spore which is cultured on a slant is produced into a single-spore suspension by using sterile water, which is also inoculated in a seed culture medium for culture, the temperature is 28 to 32 DEG C, the rotational speed is 200rpm and the culture lasts for 2 to 6 days;(3) fermentation culture: the seed liquid is inoculated in a fermentation culture medium for culture, the temperature is controlled at 28 to 37 DEG C, the pH is controlled at 6.0 to 9.0, the rotational speed is 180 to 220rpm, the fermentation lasts for 5 to 9 days, so as to obtain the Streptomyces sp., methanol or ethanol is used for the extraction and separation of mycelium through the method of centrifugal separation, then the resin method is adopted for carrying out refining, so as to obtain the peptide antibiotics enramycin.

NJWGY3665菌种接到葡萄糖营养培养基中，进行斜面培养，培养温度28-37℃，培养时间2-6天；(2)种子培养：用无菌水将斜面上培养的孢子制成单孢子悬液，并接种到种子培养基中培养，温度为28～32℃，转速为200rpm，培养2-6天；(3)发酵培养：将种子液接种到发酵培养基中培养，温度控制在28-37℃，pH控制在6.0-9.0，转速180-220rpm，发酵5-9天，得到链霉菌，采用离心分离的方法将菌丝体用甲醇或乙醇进行提取分离，再采用树脂方法进行精制，获得多肽类抗生素安来霉素。

In this paper Ⅰ successfully applied these methods to fault diagnosis, and presenting some new innovative machinery fault diagnosis method; First Ⅰ present a new gear wornout fault diagnosis method, this method use the vibration signal's time-frequency attribute and instantaneous phase undulation to accurately detect gear's fault, and by comparing with the traditional frequency domain analysis method, this method reveals that many nonlinear and nonstationary characteristic exist in gear's vibration, this tell us that if we use frequency domain analysis method without pay attention to the signal's characteristic, we could get wrong diagnostic result; Then Ⅰ present a new bearing fault diagnosis method, this method use ATVFD and Hilbert transform to do envelop demodulation,Ⅰ analyze the envelop with time-frequency method and find out that this method is more accurate then traditional method.Ⅰ also explain that the modulation of the fault to the carrier wave may also be non-stationary and nonlinear; Finally Ⅰ present a cylinder pressure measurement method of diesel engine by means of vibration, cylinder pressure is an important parameter of diesel engine fault diagnosis, the pressure measuring is simplified a lot by this method,Ⅰ also use the ATVFD and time-frequency analysis to realize it.

本文中将上述理论方法成功地应用到故障诊断中去，提出了几点创新性的诊断方法；提出了一种齿轮磨损故障诊断的新方法，该方法利用振动信号能量的时频域分布特征与瞬时相位波动特征可以进行精确的齿轮故障诊断，并通过与传统的频域诊断方法的比较，揭示出齿轮振动中存在的明显的非线性与非平稳特征，说明了不加选择地利用频域分析方法进行齿轮故障诊断很可能会得到错误的诊断结果；提出了一种轴承故障诊断的新方法，该方法利用ATVFD分解与Hilbert变换进行包络解调，并对解调得到的包络信号进行时频域分析，可以实现更加准确轴承故障诊断，说明了故障对载波信号的调制往往也是非平稳与非线性的；提出了一种利用振动信号进行柴油机汽缸内压力检测的新方法，柴油机汽缸压力的变化是柴油机故障诊断的重要参数，利用振动方法可大大简化汽缸压力的检测过程，该方法也是综合利用ATVFD分解与时频域分析方法实现的。

Based on above results, the fermentation medium and cultivation conditions were investigated and the uniform design method was used in the optimization of fermentation medium. The techniques of fed-batch culture were also investigated in this paper, with this techniques acarbose fermentation titre increased from 1416 μg/ml to 2700 μg/ml, basically reached the level of industrial production.

在此基础上，又对发酵培养基的组成及发酵条件等方面进行考察，并采用均匀设计的方法对发酵培养基的组成进行了优化；对补料等发酵工艺进行了考察，使发酵单位由原来的1416μg／ml提高到2700μg／ml，基本上达到了工业化发酵的水平。

By comparing with the curves of classical antibiotics in Doskochilova system, the antibiotic type of fermentation broths was identified. The antimicrobial tests showed, the inhibition rate of alt the twenty strains were considerably and ten of them reached 80% when the fermentation broths was diluted by 10 times. It showed strongest inhibition effect against Erwinia carotorora (17.75 mm). Organize method tests showed that inhibition rate of fermentation broths of the strain HJ1-2 to Sclerotinia sclerotiorum and Botrytis cinerea was 100% and 71.39%, respectively.

发酵液的抑菌活性测定结果表明，放线菌HJ1-2菌株发酵液对20种供试病原真菌均具有不同程度抑制作用，其10倍稀释液对其中10种病原真菌菌丝生长的抑制率达到80%以上；供试7种细菌中放线菌HJ1-2菌株发酵液对白菜软腐病菌的抑制作用最强，抑菌圈直径为17.75mn；放线菌HJ1-2菌株发酵液对油菜菌核病和番茄灰霉病的抑制率分别为100和71.39%。

By developing the optional measurement condition for ruminal fermentation products and comparing the effects of different levels of tannic acid on fermentation and methane production, the proper adding levels of tannic acid in vitro was established. We also examined the characteristics of rumen fluid fermentation and methane production of tannic acid unsupplemented or supplemented with different forage : concentrate ratios by using gas production method.

通过对瘤胃体外发酵系统中单宁酸的生物降解以及对瘤胃内环境影响的研究，初步确立了单宁酸改善瘤胃发酵的适宜添加量及其效果；并探讨了在不同精粗比底物条件下单宁酸对瘤胃体外发酵和甲烷产量的作用。

This train classifies "information carrier adhering to the build" as design elements, and probes into integrated design method of it and single building separately: method of definite position, method of definite form and method of combination; Then, this train probes into integrated design method of it and colonys building , that bases on the cognitive design method: method of rhythm controls,method of node outstanding, and that aims at the design method to protect: method of protect original, method of repairing and method of renewal.

本文的第四部分提出了一体化设计的思路，将建筑附着信息载体作为设计元素，探讨了它与建筑单体一体化设计的方法：定位法、定形法、混合法；以及它与建筑群体一体化设计时，基于认知的设计方法：节奏控制法、节点突出法，旨在保护的设计方法：存真法、修缮法、更新法。

The results showed that the optimum fermentation conditions are as follows: fermentation time 4 days, inoculum size of yeast 2% and addition of amylaceumq 8%, the alcoholicity in fermentation broth 5.33%.

在单因素试验的基础上，用正交试验对五味子发酵工艺条件进行筛选，得出最优发酵条件为：发酵时间为4d，酵母菌接种量为0.2%，葡萄糖加入量为8%，发酵原液酒精度达到5.33%。

Diacetyl is signal products of ripeness of beer fermentation and proper content of higher alcohols could make beer products full and mild in taste, but excessive higher alcohols not only bring beer peculiar smell but also make beer toxic and ebriety caused by alcohols, so superscale of diacetyl and higher alcohols had become restrictive factors and outstanding contradictions of improving beer qualities, especially in producing low cost beer products fermented by elevating fermentation temperature, shortening fermentation period and increasing content of subsidiary materials.

双乙酰也是啤酒发酵成熟的标志物质，过量的双乙酰给啤酒带来不愉快的"馊饭味"，而适量的高级醇可使酒体丰满圆润、口感柔和协调，但高级醇含量过高，不仅会给啤酒带来异杂味，还易发生醇类中毒，导致醉酒。因此，双乙酰和高级醇超量已成为制约啤酒质量的重要因素，特别是在提高温度、缩短周期、增加辅料的低成本快速发酵中，这一问题更加突出。

Three species of macrophyte (Elodea nuttalli, Vallinmeria spiralis, Alterranthera philoxerides), which are widely distributed in water environments, were used to perform solid-state fermentation to produce crude proteins. The effects of single-strain fermentation and mixed-strains fermentation of aquatic macrophyte on yields of their crude proteins and cellulase activities were analyzed.

采用微生物固体发酵技术对伊乐藻、苦草和喜旱莲子草3种高等水生植物进行单细胞蛋白生产的研究，分析单一菌种和混合菌种发酵过程中水生植物粗蛋白含量和纤维素酶活的变化过程。

Fostering the concept of multi-culture firmly and mastering the development of advanced culture correctly are conductive to resisting terrorism, separatism of nationalities and ultraism of religion, which is of great academic theoretical, immediate and profound historical si...

牢固树立多元文化的理念和正确把握先进文化的发展方向，有助于在世界范围内自觉地抵制恐怖主义，民族分裂主义和宗教极端主义。不仅具有重大的学术理论意义，而且也具有重大的现实意义和深远的历史意义。

But if anyone walks in the night, he stumbles, because the light is not in him.

11：10 若在黑夜行走，就必碰跌，因为那光不在他里面。