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Secondly, Bifidobacterium medium was developed using JAJ as main material. The results showed that: Adding Tryptone,soy peptone.beef extract,com extract and Cys-HCl to JAJ could obviously promote the growth of Blm and Bbm;By the orthogonal experiment of three elements and three levels,a satisfying JAJ compound medium was acquired which included corn extract(0.3%), soy peptone(0.05%)and Cys-HCl(0.025%).Nextly,after establishing a selective Bifidobacterium medium, the effects of JAJ on the growth of Bifidobacteria in vivo were studied,using healthy mouse of Kunming species as experimental animal.

研究了以菊芋汁为主要原料的双歧杆菌培养基,大量试验结果表明,在菊芋汁中添加胰蛋白胨、牛肉膏、大豆蛋白胨、玉米浆和半胱氨酸盐酸盐等成分,对双歧杆菌有明显的促进生长作用;利用大豆蛋白胨、玉米浆和半胱氨酸盐酸盐设计了三因素三水平的正交试验,确定了菊芋汁复合培养基的优化配方:菊芋汁+0.3%玉米浆+0.05%大豆蛋白胨+0.025%半胱氨酸盐酸盐。

He experiment results indicate that (1) The extract of Rubia Cordifolia L has the effects of anti-proliferation and inducing APO;(2) Whenadministrating with 5-fu, the extract of Rubia Cordifolia L obviously enhanced the effects of anti-proliferation and inducing APO of 5-fu;(3) The basement of molecular biology of the extract of Rubia Cordifolia L for inducing APO of MGC-803 cells is correlated with inhibiting the expression of bcl-2 of anti-apoptosis gene.

UNEL法与PCNA的免疫细胞化学法的结果:0.2、0.4、0.8、1.0mg/ml浓度的茜草提取物与2、4、8、16μg/ml浓度的5-Fu,分别诱导细胞凋亡和抑制细胞增殖,表现出浓度、时间依赖性关系,除个别浓度与个别时间之间无显著性差异(P>0.05)外,不同浓度与不同作用时间之间均有显著性差异(P<0.05或P<0.01),并且两种药物联合使用时,MGC-803细胞的APO%显著提高,降低PCNA的阳性表达率(P<0.05或P<0.01)。

The results showed that 0.01,0.05 and 0.10 g/ml extract of Saxifraga stolonifera Meerb had strong antimicrobial effect on S.aureus,but 0.25 and 0.50 g/ml extract had promotive effect on S.aureus.And the extract of Saxifraga stolonifera Meerb of different concentration had antimicrobial effect on E.coli.

结果表明,0.01、0.05、0.10g/ml 3种较低浓度的提取物对摇床培养的金黄色葡萄球菌生长具有较强的抑制作用,而0.25和0.50g/ml 2种较高浓度的提取物对摇床培养的金黄色葡萄球菌生长有较强的促进作用;这5种浓度的提取物均对摇床培养的大肠杆菌生长表现为抑制作用。

Method] Alcohol solvent extraction method was used to extract the total extract of Thermopsis lanceolata, and it was treated by acid-soluble and alkalization. Then chloroform was used to extract the total alkaloids. Finally, GC-MS was used to analyze and identify the chemical composition.

方法]采用醇类溶剂提取法,提出披针叶黄华总浸膏,经酸溶、碱化处理,再用氯仿萃取出总生物碱,利用GC-MS法对其化学成分进行分析鉴定。

On the inhibition to mycelial growth, the 95% ethanol extract had significantly inhibition to P. ultimum, F. oxysporum f. sp cucumber, R. solani and B. cinerea, and at the top treatment concentration of 0.002 g/mL, the inhibition rate reached to 60.32% 58.50%, 55.89% and 55.17%, respectively; But the 95% ethanol extract almost had no inhibition to S. rolfsii, its rate was just 17.19% at 0.002 g/mL.③On the inhibition to sprouting, the 95% ethanol extract had significantly inhibition to B. cinerea, R. solani, F. oxysporum f. sp cucumber and P. ultimum, and at the top treatment concentration of 0.002 g/mL, the inhibition rate reached to 95.24%, 90.48%, 89.47% and 85.71%, respectively; and the rate would exceed 40% to four fungi mentioned above when their concentration was not lower to 0.005g/mL. The 95% ethanol extracthad infirm inhibition to S. rolfsii, its rate was just 43.48% at 0.002 g/mL.

在抑制菌丝生长方面,鱼腥草95%醇提物对豌豆根腐病菌、黄瓜枯萎病菌、马铃薯立枯丝核病菌和大白菜灰霉病菌均有良好的抑制作用,在最高处理浓度为0.002 g/mL时抑制率分别达到60.32%、58.50%、55.89%和55.17%;对辣椒白绢病菌几乎没有抑制作用,在最高处理浓度为0.002 g/mL时抑制率仅为17.19%;③在抑制孢子萌发方面,鱼腥草95%醇提物对大白菜灰霉病菌、马铃薯立枯丝核病菌、黄瓜枯萎病菌和豌豆根腐病菌抑制效果明显,在最高处理浓度为0.002 g/mL时的抑制率分别达到95.24%、90.48%、89.47%和85.71%,且在处理浓度不低于0.000 5 g/mL时对以上4种真菌孢子的抑制率超过40%;对辣椒白绢病菌抑制作用较弱,在最高处理浓度为0.002 g/mL时仅为43.48%。

Catarium that is naturally decomposed outdoors has an inhibitive effect on cabbage seed germination. While the inhibition effect increases with increase in extract concentration, it, however, decreases with decomposition time. With regards to the length of cabbage root, 0.1 g·mL-l aquatic extract of E. catarium inhibits root growth at every decomposition stage. With the exception of undecomposed E. catarium , other concentration extracts during decomposition enhances root growth. Aquatic extract from E. catarium also enhances cabbage stem growth.

滤纸培养皿试验结果表明:假臭草植株提取液对小白菜种子发芽的抑制作用随浓度增大而增大,且随植株分解时间的延长而逐渐减弱;就幼苗根长而言,不同分解时期0.1 g·mL-l的假臭草提取液对根长均表现为抑制作用,除未分解的假臭草以外,其他时期其他浓度的假臭草提取液对根系生长均有促进作用;假臭草提取液对小白菜茎长总体表现为促进作用。

The specific gravity of degassed and undistilled beer sample plus real extract solution are determined in this method.The results of alcohol,real extract,original extract and real degree of attenuation etc.can be shown through the running of software program.

摘 要:通过测定脱气而不蒸馏的啤酒样品及浸出物溶液的比重,在开发的软件程序上运行后可得出酒精度、真正浓度、原麦汁浓度及真正发酵度等参数结果,该方法不需额外实验室硬件投入,比GB4928—91标准中蒸馏法测酒精度及原麦汁浓度含量提高效率8倍。

Unhydrolyzed extract is difficult to be utilized by fungi used in the experiment because the Arabinogalactan is main composition in the extract, but the fungi can use the sugars in hy-drolyzed extract with the addition of Nitrogen, phosphrus, etc.

未经水解的提取液主要含有阿拉伯-半乳聚糖,难以被试验菌种利用;水解后添加必要的氮、磷等营养物质,被试菌种可利用其中的单糖生产单细胞蛋白。

The results showed that The ratio of the compositions of the two extracts are different, and the extract rate of the main chemical substances are high in extract decocted together than in extract decocted single.

结果表明,加醇合煎、水合煎、水分煎的提取物均未发现明显新峰,但三者各共有峰相对积分面积存在差异,加醇合煎和水合煎提取物中主要化学成分的提取率大于水分煎提取物,且共有化学成分的比例也不同。

The higher the concentration, the bigger the physiological index. 3. The Ether extract, the n-BuOH extract, the acetone and the chloroform extract treatment after chilling stress and restored the growth, the content of protein, the content of chlorophyll, SOD、POD and CAT activities were higher compared the control, the content of MDA was lower compared the control.

经前胡乙醚、正丁醇、丙酮和三氯甲烷提取物质喷雾处理的幼苗在低温处理36小时后,可溶性蛋白质含量、SOD、POD、CAT活性均下降,在低温处理恢复生长后,SOD、POD、CAT、可溶性蛋白和总叶绿素含量均比低温对照幼苗高,MDA含量比低温对照幼苗低;在低温处理后及恢复生长后各生理指标随浓度的增加呈现出先升高后降低的趋势。

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