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And proved that there is CBF gene expression-regulated pathway and transcriptional cascade leading to the expression of cold-responsive genes under cold stress in Capsella bursa-pastoris, which Cbice53 activate the expression of Cbcbf and Cbcbf activate transcription of Cbcor15b and the CbLos2 had a indirect and important regulatory function in controlling cold-responsive genes expression under low temperature like the same pathway in Arabidopsis thaliana.

证明了在荠菜中存在Cbice53激活Cbcbf的转录，Cbcbf激活Cbcor15b的转录，同时CbLos2对Cbcor15b的转录有间接的正向调控作用的抗冷胁迫基因表达级联机制，即存在与拟南芥相类似的抗冷胁迫的CBF基因表达调控途径。

Results］ the expression of cox-2 in hepatocellular carcinoma and its adjacent tissues with cirrhosis were 70.0% and 92.0% respectively, and they were higher than that in normal liver tissue (20.0%). the cox-2 expression in carcinoma adjacent tissues with cirrhosis was higher than that in hcc. expression of cox-2 in well differential hcc was higher than that in poor differential hcc, and higher in case with metastasis than that without metastasis(p＜0.01).the expression of cox-2 in hcc did not relate to tumor size, tumor capsula and the level of afp.

结果］ cox-2蛋白在hcc、癌旁肝硬化中表达阳性率分别为70.0%和92.0%，显著高于在正常肝组织中的20.0%（p＜0.05），而cox-2在癌旁肝硬化组织中的表达高于hcc组织。cox-2蛋白在分化好hcc中的表达显著高于分化差的hcc（p＜0.05），转移组中cox-2蛋白表达显著高于无转移组（p＜0.01）；而cox-2蛋白的表达与肿瘤大小、有无包膜及afp水平无关。

Results The levels of HSP 70 mRNA and HSP 70 protein expression were significantly different among the 5 groups. Ketamine induced marked HSP 70 mRNA and HSP 70 protein expression in the posterior cingulated cortex. Propofol itself did not induce HSP 70 gene expression in this brain region. Propofol significantly inhibited ketamine-induced HSP 70 mRNA and HSP 70 protein expression in the posterior cingulate cortex in a dose-dependent manner.

结果氯胺酮可明显诱导HSP70 mRNA与HSP70蛋白在大鼠后扣带回皮质区的表达；异丙酚自身不能诱导HSP70基因的表达；预先给予异丙酚可显著抑制氯胺酮诱导的HSP70 mRNA和HSP70蛋白在这一区域的表达，且抑制效应呈剂量依赖性。

In order to investigate the role of ICAM-1 and VCAM-1 in the pathogenesis of glomerulonephritis, ICAM-1 and VCAM-1 expression were evaluated from both protein and gene level by in vivo and in vitro study, We have conducted (1) immunocytochemical analysis and in situ hybridization to examine the alteration in expression of ICAM-1 and it's relationship with interstitial infiltrating cells and TNFα; A total of 64 renal biopsies were classified in three groups according to the degree of cellular proliferation and infiltration in glomerulus;(2) indirect immunofluoresence and immunoblotting. methods to detect the VCAM-1 level both in renal tissue and in serum from the patients of lupus nephritis (17cases) and crescentic nephritis (4 cases);(3) Cell ELISA and northern blot technique to study the effects of TNFα and IL-1β on ICAM-1 and VCAM-1 surface expression and gene expression by cultured human mesangial cells .

为了探讨ICAM-1和VCAM-1在肾小球疾病中的作用，本文从蛋白质和基因两个水平，整体（研究ICAM-1时根据肾小球内细胞增生和浸润程度，将64例病人分为A.B.C三组）和细胞培养两个方面，做了如下工作，（1）利用免疫细胞化学和原位杂交技术观察了ICAM-1在64例肾小球疾病患者中的表达及其与间质浸润细胞、TNFα之间的关系；（2）利用间接免疫荧光和膜免疫印迹方法检测了VCAM-1在17例狼疮肾炎和4例新月体肾炎病人肾组织及血清中的表达；（3）利用细胞ELISA和Northern杂交技术研究了IL-1β或TNFα对体外培养的人肾小球系膜细胞ICAM-1和／或VCAM-1表面表达及mRNA表达的调节作用。

As with rhIL-1β, rhTNFα(100ng/ ml) can also provoked ICAM-1 surface expression by HMC. We also found VCAM-1 mRNA expression by HMC in normal culture condition and exposure of mesangial cells to rhTNFαis associated with increased VCAM-1 mRNA lavels rapidly (4 hours).These exploration indicate (1) glomerular and tubular ICAM-1 expression level correlate with inflammatory degree of glomerulus and TNF-αexpression level (2) VCAM-1 may have certain role in patients of lupus nephritis and crescentic nephritis (3) Expression of ICAM-1 and VCAM-1 may be regulated in vivo by cytokines (TNFα and IL-1β).

与轻度系膜增生性肾炎病人血清中的VCAM-1水平比较狼疮肾炎病人血清中VCAM-1的水平显著增加；（3）rhIL-1β（25ng/ml）不但能增加ICAM-1 mRNA的表达，还增加ICAM-1蛋白的表达；rhTNFα（100ng／ml）对ICAM-1蛋白表达和VCAM-1 mRNA基因表达均有明显的上调作用，结果提示：肾小球和肾小管ICAM-1表达水平与肾小球炎症病变程度有关；VCAM-1在狼疮肾炎和新月体肾炎的发生发展中可能有重要作用；体内ICAM-1和VCAM-1的表达可能受TNFα和IL-1β的调节。

The research work involving the expression manner of doxA gene in S. lividans TK24 with plasmid pYG57 implied that the promoter PermE may control its expression constitutively, the time for maximum expression emerged from 48 to 60 h after inoculation and cultivation and the expression level was kept relatively stable, furthermore, the recombinant hydroxylase existed mostly in mycelium cell but little in broth.

而对Tm4（pYG57）菌株中doxA基因表达方式的研究表明， dd基因在 PermE启动子控制下可能是一种组成型控制表达，其表达量在接种后培养到 48－60 h左右最高并且维持相对稳定，并且表达的柔红霉素 Cl4羟化酶主要存在于菌丝体细胞内，很少分泌到细胞外。

Expression of nNOS mRNA, eNOS mRNA and iNOS mRNA in the E 20 cerebral cortex: There were no obvious changes of expression of nNOS mRNA in ETU-injected and control groups. Expression of eNOS mRNA increased significantly in cerebral cortex of the encephalocoele group, And there were no obvious changes of expression of eNOS mRNA in cerebral cortex of the control group and the other ETU-injection groups.

六、不同组E20胎鼠大脑皮质中nNOS mRNA、eNOS mRNA和iNOS mRNA的表达 1、实时定量PCR检测发现对照组、给药无畸形组、脊柱裂组和脑膨出组E20胎鼠大脑皮质中nNOS mRNA表达无明显改变； 2、给药无畸形组、脊柱裂组和对照组胎鼠大脑皮质中eNOSmRNA表达无明显改变，而脑膨出组胎鼠大脑皮质中eNOS mRNA表达明显增多； 3、在各组未检测到iNOSmRNA表达。

Objective To study the relationship of endangium proliferation and the expression of metalloproteinases and tissue inhibitors of metalloproteinases after angioplasty of iliac artery in rabbits to investigate the probable mechanism of composite Danshen pill in prevention and treatment of restenosisMethods 30 white male rabbits of Japan （the average body weight was 25～30 kg）were randomly divided into 3 groups:normal control group,model group (intima destroyed by balloon and given hypercholesterol diet),CDP group (intima destroyed by balloon and given hypercholesterol diet plus drug CDP 150 mg/d),10 ones each groupResults The results of iliac artery angiogram and the analysis of pathology:there were lumens stenosis,thinner intima,smaller intima area,in CDP group compared with those in model group,and ratio of intima and tunica media thickness and area among each group (P＜001)Immunohistochemistry analysis:the MMP2,MMP9,TIMP1,TIMP2 in model group significantly increased than those in control group (P＜001)The CDP group had lower MMP2 and MMP9 expression,higher TIMP1 and TIMP2 expression,and increased MMP2/TIMP1,MMP9/TIMP2 than model group (P＜001)The thickening of vascular neointima and stenosis degree of vascular lumen were relevant to MMP2 and MMP9 (r=0896,P＜001)Conclusions MMP and TIMP play the very important role in the restenosis process of arteryCDP could inhibit the thickness of vascular neointima after balloon injury,the probable mechanism of which may be inhibiting collagen formation,smooth muscle immigration and decreasing hyperplasia of intima by interfering expression of MMPs and TIMPs

目的 研究兔髂动脉成形术后血管内膜增生和基质金属蛋白酶及其抑制物表达之间的关系，探讨复方丹参滴丸预防再狭窄的可能机制。方法健康成年二级雄性日本大耳白兔30只，平均体重25～30 kg。随机分为3组：正常对照组10只，模型组10只（球囊内膜剥脱加高胆固醇饮食），治疗组10只（球囊内膜剥脱加高胆固醇饮食以及复方丹参滴丸150 mg/d）。结果兔髂动脉造影、血管病理图像分析检测结果：①复方丹参滴丸组较模型组血管造影示管腔直径狭窄、内膜厚度减少、内膜面积减少、内膜厚度和中膜厚度比、面积比，各组之间有显著性差异(P＜001)。②免疫组化分析：模型组MMP2、MMP9、TIMP1、TIMP2表达均高于对照组（P＜001）；复方丹参滴丸组MMP2、MMP9的表达低于模型组，而TIMP1、TIMP2的表达高于模型组；TIMP1/MMP2、TIMP2/MMP9明显增加，差异有显著性（P＜001）。③内膜的增生以及管腔的狭窄程度与MMP2、MMP9有很好的相关性(r=0896，P＜001)。结论 MMP和TIMP在再狭窄形成中起重要作用；复方丹参滴丸能够明显抑制血管损伤后的内膜增生，可能的机制是通过影响金属蛋白酶MMP2、MMP9及其抑制物TIMP1、TIMP2的表达从而抑制胶原的生成、平滑肌的迁移、增生，而减少内膜的增生。

Theurinary IL-6 level positively correlated with density of glomerular matrix membrane, global sclerosis, fiber or fibrocellular crescents and interstitial fibrosis (p. 05). According to the degree of density of glomerular matrix membrane and interstitial fibrosis, urinary Col-IV level had better correlation than urinary TGF-betal and IL-6 levels.In IgAN, Col-IV showed increased expression in diseased renal tissue whereas the site of expression of TGF-betal was mainly localized within the cytoplasm of tubular epithelial cells. Interstial expressionwas also present but glomerular TGF-betal expression was found only in patients with heavy mesangial proliferation. There was a significant correlation between glomerular positivity for Col-IV and severity of histological damage. There was also a significant correlation between positivity for TGF-betal and Col-IV in the tubular epithelial and interstitial lesions. In contrast, there was no ralationship between glomerular positivity for TGF-betal and severity of histological damage.The urinary TGF-betal level paralleled tubular TGF-betal expression.

结果 ①IgAN患者尿TGF-β1、IL-6、Col-Ⅳ水平较健康人明显增高(P＜0.01)，该变化与血中的浓度无关(P＞0.05)；②尿TGF-β1水平与小管间质TGF-β1阳性表达呈正相关(P=0.000)，而与小球TGF-β1阳性表达无关(P＞0.05)，尿Col-Ⅳ水平与小球和小管间质Col-Ⅳ阳性表达均呈良好的相关性(P＜0.01)，还与小管间质TGF-β1阳性表达呈正相关(P＜0.05)：③小球Col-Ⅳ阳性表达与肾组织慢性病变密切相关(P＜0.05)，小管间质Col-Ⅳ和TGF-β1阳性表达均与肾小管间质病变呈良好的相关性(P＜0.01)，而小球TGF-β1阳性表达与肾组织损伤无关(P＞0.05)；④尿TGF-β1、Col-Ⅳ水平与肾小球基质基底膜面密度、小管间质病变呈正相关(P＜0.01)，与小球内细胞数呈负相关(P＜0.05)，该结果与其在组织中的表达一致；尿IL-6水平浙江大学硕士学位论文尿TGF一B一、IL一6和Col一IV在IgA肾病中的应用价值与基质基底膜面密度、球性硬化、纤维或细胞纤维新月体所占肾小球百分数及小管间质病变均有显著的相关性(F.05)；在轻度肾病理损伤时，尿'l'G卜pl、I卜6、Col一IV水平即升高，而尿Col一W在反映细胞外基质积聚和间质纤维化程度上比尿TGF一pl和IL一6有更好的相关性。

The mRNA expression of MMP-2 in more than 4 lymphnodes positive is lower than in lymphnode 1-3 positive （P＜0.05）.The MMP-2 mRNA expression is irrelated with tissue grade,pathologic type,ER,PR and Her-2.Conclusion MMP-2 mRNA expression is upregulated in early clinical stage,lymph node metastatic early stage,and down regulated with tumor progressing.MMP-2 mRNA expression is related with the metastasis of breast cancer.

MMP-2 mRNA转录水平的表达与乳腺癌转移有关，MMP-2在转录水平的表达在肿瘤较小时上调，随着肿瘤的增其转录水平表达下调，随着肿瘤恶性程度的增加，其转录水平的表达量在下降，在肿瘤发生的早期表达上调，随着肿瘤的进展表达量下调。

Chimborazo and Cotopaxi, took me by the hand.

越过琴博腊索山和科托帕克西山。

This car is in a good condition.

这辆车的状况很好。