查询词典 expression

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

The expression of AT2R gene can be regulated efficiently in vivo by local delivery of MSC with dual-stable expression of AT2R gene, thus reduces the formation of intimal hyperplasia.④AT2R gene can influence the expression of ECM and significantly promote the cellular apoptosis in the injury rat carotid artery.⑤These data demonstrate the clinical potential of AT2R regulatable expression to prevent restenosis after PCI.

研究结论：(1)与VSMC直接接触可以诱导MSC向血管成分细胞分化；(2)构建的Dox-on可调控的MSC细胞株诱导活性可靠，使外源AT2R基因的表达处于有效的主动控制下；(3)局部导入MSC不影响球囊损伤后大鼠颈动脉新生内膜增生；(4)由MSC介导的AT2R可调控表达受到Dox的良好调控，可有效抑制球囊损伤后大鼠颈动脉新生内膜增生；(5)AT2R影响血管损伤后修复时ECM成分的表达，并促进细胞凋亡的发生，可能是AT2R介导抑制新生内膜形成的机制。

Deduced by the proportion of typical sterile plants and normal fertile plants in segregative generations derived from WA zhenqiuA/6078 that:I gene could inhibit the expression of Rf gene completely by the heterozygosity of 1 pair of Rf in genotype;and only reduce the expression of Rf genes with two heterozygous Rf genes in genotype ;but it would never inhibit the expression of Rf genes if the genotype included 3 pairs of heterozygous Rf genes. When Rf gene was homozygous,the / gene could not inhibit the expression of it.

根据认叭真秋刀6078各衍生分离世代中典型不育株和正常可育株所占比例推断：基因型中仅包含1对杂合的Rf基因时，I对Rf的表达起完全抑制作用；基因型中包含2对杂合的Rf基因时，I仅对Rf的表达起削弱作用；当基因型中包含3对杂合的对基因时，I对Rf的表达不起抑制作用；I对纯合位点的Rf的表达不起抑制作用。

Estrogen deficiency can increase the mRNA expression of MMP-1、MMP-3 and IL-1β in OA synovia tissue. Estrogen could inhibit the mRNA expression of MMP-1、 MMP-3 and IL-1β in OA synovia tissue. Progestin could even decrease this expression. Estrogen could slightly increase the mRNA expression of TIMP-1. It is suggested that low dose of estrogen supplement play a protective effect on OA cartilage, and progestin could increase this protective effect.

雌激素缺乏可使OA关节滑膜中MMP-1、MMP-3和IL-1β mRNA表达增加，雌激素对关节滑膜组织中MMP-1、MMP-3和IL-1β mRNA表达有抑制作用，孕激素可增强雌激素的这一抑制作用，雌激素对TIMP-1有轻度升高作用，提示低剂量雌激素对OA关节软骨有一定保护作用，而加用孕激素这一保护作用增强。

A parenthesized expression list yields whatever that expression list yields: if the list contains at least one comma, it yields a tuple; otherwise, it yields the single expression that makes up the expression list.

括号内表达式列表的结果取决于其内部表达式列表的结果：如果表达式列表中包括至少一个逗号，它就生成一个元组；否则，就生成一个由表达式列表组成的表达式。

Results:(1) The rats produced amphetamine CPP after treated with 2 mgkg^(-1) amphetamine for 4 days. Ketamine and low, middle, high dose rhynchophylline all could eliminate the CPP effect, and the effect of rhychophylline was increased in a dose-dependent manner. Rhychophylline itself could not induce CPP in normal rats.(2) When compared with normal rats, the NR2B expression in nucleus accubens and amygdaloid of the model rats was obviously increased. Ketamine and middle, high dose rhynchophylline could decrease NR2B expression induced by amphetamine, while low dose rhynchophylline did not affect NR2B expression. Rhynchophylline had no effect on RN2B expression in normal rats.

结果：(1)建立了苯丙胺（2mgkg^(-1)，连续4d）诱导的位置偏爱模型，氯胺酮及钩藤碱低、中、高剂量均可消除苯丙胺诱导的位置偏爱效应，随钩藤碱剂量增加其效应加强，且本身无精神依赖性；(2)苯丙胺模型组大鼠伏核和杏仁核NR2B蛋白表达增加，氯胺酮及钩藤碱中、高剂量抑制NR2B表达，低剂量及本身对NR2B表达无影响。

In the later stage of infection, morphine increase the expression of p53, Bax and surpress the expression of bcl-2; whereas methionine enkephalin elevate the expression of bcl-2 and surpress the expression of Bax and caspase.

在感染晚期，吗啡可增加细胞p53、Bax、caspase表达，降低bcl-2表达；而蛋脑啡肽可增加bcl-2表达，降低Bax、caspase表达。

The aortic tunica intima was intact, the number of foam cells was decreased, and inflammatory cells infiltrated into the med-intima smooth muscle of the n-3 PUFA group. However, with no such changes were observed in control and n-3 PUFAs groups. Little or no expression of NF-κB, ICAM-1 and MCP-1, and a high expression level of IκBα were detected in aortic tunica intima of the n-3 PUFA and control groups, while high expression levels of NF-κB, ICAM-1 and MCP-1, and little expression of IκBα were detected in aortic tunica intima of the high fat-fed group.

结果 n-3脂肪酸组大鼠体重第1~11周均明显降低，空腹血糖、血清胰岛素、TG、TC、LDL-C、总FFA和内脏脂肪占体重百分比均明显低于HF组，HDL-C则明显高于HF组，与对照组无明显差别；n-3脂肪酸组大鼠主动脉主动脉内膜完整无内皮细胞脱落现象，内膜下泡沫细胞及炎细胞浸润减少，内膜的NF-κB呈弱阳性表达，IκBα呈阳性表达，ICAM-1和MCP-1均呈阴性或弱阳性表达。

objective:to investigate the expression and significance of rb in the occurrence, development and regression of infantile hemangiomas.methods:the expression of rb was examined in the proliferative stage and catagen of human hemangiomas and normal skin tissues by using immunohistochemical technique.immunohistochemical technique for factorⅷ-related antigen was used to prove that the cells which expressed rb were endothelium.image analysis system was applied to measure the expression level of rb at different stages of hemangiomas and in normal skin tissues.results:the expression of rb was significantly lower in proliferating hemangiomas than that in involuting hemangomas(p.05).conclusion:rb might play an important role in the regression of human hemangioma endothelial cells and anti-angiogenesis.

目的：探讨rb蛋白在血管瘤发生、发展及退化过程中的表达状况及其意义。方法：采用免疫组织化学方法检测人皮肤血管瘤增生期、退化期及正常皮肤组织中rb的表达水平，并结合第ⅷ因子相关抗原的免疫组织化学染色证实表达rb的细胞是血管内皮细胞。利用计算机图像分析技术测量不同时期血管瘤组织和正常皮肤组织rb表达的积分光密度和面积。结果：增生期血管瘤内皮细胞rb表达水平低于退化期，差异有显著性（p.05），退化期血管瘤内皮细胞rb表达水平与正常皮肤组织相比，差异有显著性（p.05）。结论：rb通过抑制血管瘤内皮细胞增殖和血管生成而在血管瘤的退化过程中起重要作用。

These results suggested that the function of each function domain in HIV 1 LTR exhibited distinct characteristics on gag gene expression in vaccinia virus:(1) The function of intact LTR on gag gene expression varied with the upstream poxvirus promoter;(2) NR sequence neither decreased nor increased the Gag protein exprssion level;(3) Enhancer sequence might not be recognized by recombinant vaccinia virus expression system;(4) TAR sequence enhanced Gag protein expression effectively;(5) U5 region and its downstream non translated sequence had little effect on

免疫印迹和免疫酶试验检测均表明，6株重组病毒的Gag蛋白表达量因LTR不同而有明显差异，表明HIV1的LTR及其下游基因置于痘病毒启动子控制下，在痘苗病毒中表达时有下述特点：（1）不同的痘苗病毒启动子与全长LTR相互作用，对gag基因表达有显著不同的调控效果；（2）NR序列对Gag蛋白表达没有明显影响；（3）EN序列不能被重组痘苗病毒表达系统识别；（4）TAR序列可提高Gag蛋白的表达量；（5）U5区及下游非翻译序列不影响Gag蛋白的表达。

Methods: Five patients with parkinsonism or dystonia were assigned to general anesthesia using an modified endotracheal tube.

本实验依照人体实验之相关规定进行，五位患有帕金森氏症或肌张力异常的病人接受神经立体定位手术。

If you can benefit from this book, it is our honour.

如果您能从本书获益，这将是我们的荣幸。