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The expression of FS mRNA in granulose cells of small atresic follicles is stronger. Its suggested that the abnormal expression of human FS mRNA is most potentially associated with the etiology of PCOS.

与非PCOS妇女相比，FSmRNA在PCOS患者卵巢闭锁卵泡颗粒细胞中的表达增强，提示FS mRNA的异常表达可能与PCOS的发病相关。

The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因，并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列，通过对克隆的降钙素序列的比较研究，结果显示同一科的鱼降钙素序列保守性较高，同时，根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究，结果显示在分类学上，分类地位较远的鱼，其降钙素相似性较低，分类地位越接近的鱼，其降钙素相似性越高；我们利用谷胱甘肽S-转移酶(Glutathions S-transferase，GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究，应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索，并将纯化的融合蛋白作为抗原，获得了高效价的兔抗鲑鱼降钙素免疫血清；生物活性研究表明，大肠杆菌表达的融合蛋白具有显著的降血钙作用。

Based on expression (8), the removal distance of the crushing station is 536 m Based on expression (13), the relation between the haulage cost and the removal distance can also be obtained Fig.

根据表达式（8）破碎站的转移距离是536米。根据表达式（13）可以得到转移距离的运输成本和转移距离之间的关系（如图3）。

The HSP70 gene expression was determined in hepatopancreas, spleen, heart and head kidney in green swordtail Xiphophorus helleri challenged by Vibrio alginolyticus 0, 2, 5, 8 and 11 days after administration using RT-PCR. Results showed that tile gene expression of the HSP70 was of tissue specific.

用溶藻弧菌Vibrio alginolyticus感染剑尾鱼Xiphophorus helleri，取感染后第0、2、5、8、11 d的活鱼及感染后第2d濒死鱼，采用半定量RT-PCR方法分别检测HSP70基因在肝胰脏、脾脏、头肾和心脏组织中的表达。

The significance and the expression changes of slit-2 during the spinal cord injury of rats Objective: To investigate the ethology, morphology and the expression of axon guidance cue slit-2 mRNA changes in different phases of spinal cord hemisection injury, and to explore the effect of slit-2 in axon regeneration of the mammalian central nervous system injury.

目的：观察大鼠脊髓损伤后行为学及形态学的变化特点，同时研究轴突导向因子slit-2的表达变化及意义，进一步探讨slit-2在成年哺乳动物脊髓损伤修复和神经再生中的作用，为临床研究神经系统疾病及其治疗提供新的思路和理论指导。

Results The hind limb function of the injured rats recovered at different degrees, the most extent recovery occurred during the time site 1~2 week, recovery continued from 2 to 3 week and, BBB was up to 12 at the end of the third week, but there was no significance recovery during 3~4 week. the astrocyte caudal to the injury plane began hyperplasy and hypertrophy; astrocyte in which GFAP in expression was positive the gray matter increased obviously from 3 days to 14 days after hemisection. The expression of MBP is same as that of GFAP.

结果 伤后后肢均有不同程度的恢复，1~2周时恢复幅度最大，2~3周时后肢运动功能继续恢复，3周时BBB评分最高达12分，3~4周运动功能无显著性恢复，损伤后1 d损伤远端3~6 mm处GFAP阳性星形胶质细胞开始增生肥大，3~4 d灰质中星形胶质细胞明显增多，2周时达到高峰，损伤近端3~6 mm处少突胶质细胞的增生反应过程与星形胶质细胞相似。

If 〓mRNA positive expression, its DNA heteroploid type display as triploid or hypodiploidy, if 〓mRNA negative expression, its DNA heteroploid type display as hyperdiploidy、tetraploid or Bistem line. These from one flank demonstrate the role of 〓 gene suppress the proliferation of malignant tumor

表达阳性者，其DNA异倍体主要表现为三倍体或低二倍体，〓表达阴性者，其DNA异倍体主要表现为高倍体、四倍体和双干系，从一个侧面显示出〓基因的抑制恶性肿瘤细胞增殖的作用。

Of them are treated with immunohistochemistry modus to detect Ki-67 expression; 4. Use SPSS13.0 for windows statistics software to chisquare inspection. Results: through detecting, heteroploid pecentage and Ki-67 positive expression of the patient who suffered breast cancer grouped in the zhengqi-weak-evil-strong type is the highest in the three typies(69.4%,94.4%).

结果：乳腺癌患者术前辨证为正虚毒炽者DNA倍体异倍体、Ki-67阳性出现率最高(69.4%，94.4%)，冲任失调组次之(41.0%，58.3%)，肝郁痰凝组最低(23.5%，12.5%)，经统计学处理，三组之间有显著差异(P.05)；正虚毒炽组S期细胞比例为高度的几率最高(58.3%)，冲任失调组和肝郁痰凝组无显著差异(17.9%，23.2%)。

Results Compared to those before treatment the volume of the implanted keloid of Group D began to decrease since 14 days after treatment time-dependently (all P 《 0.05), and the volumes of the other 3 groups continued to increase and peaked on days 21, 14, or 7 respectively (all P 《 0.05).Microscopy showed infiltration of a larger quantity of histiocyte in the keloid tissue, and more obvious collagen disorganization and apoptosis of fibroblasts in Group D than in the other 3 groups.The protein expression of Bcl-2 was more remarkable and the protein expression of BAX was less remarkable in Group D than iu the other 3 groups.

结果 用药前和用药后7、14、21、28、35 d，D组瘢痕疙瘩组织块体积(mm3)分别为173±5、172±5、147±5、125±6、112±7和84±9，从用药后14 d开始明显缩小(均P＜0.05)；而其他3组瘢痕疙瘩组织块体积均明显增大，从用约后7 d开始各时点测得的体积均明显大于D组(均P＜0.05)。D组瘢痕疙瘩组织中有大量小鼠组织细胞浸润，胶原结构破坏和成纤维细胞凋亡明显重于其他3组，Bcl-2蛋门质表达明显弱于而Bax蛋白质表达明显强于其他3组。

To observe histogenesis of human kidney and pattern of PAX2 expression in fetal kidney, in order to facilitate further analysis of the relationship between PAX2 expression and kidney development.

通过观察人类肾组织形态发生和PAX2在发育肾的表达模式，以了解肾正常形态发生与其功能的联系，认识PAX2在肾发生中所起的重要作用。

For the head-teacher of the class said that I had seriously broken the school rules,which led me to a three-day suspend class.

为什么呢？因为我的班主任说我严重的违反了校规，于是让我停课三天。

Many of them believe that the conversion of thousands of working-class folk in England spared that nation from the mass carnage and the tyranny that came with the revolution in France.

他们之中有许多人相信，在英国数以千计的劳工阶级之悔改信主使英国免於遭受如法国大革命所造成的大屠杀和专制暴政。