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RESULTS We successfully obtained human caspase-3 gene, the genes and expression vectors of reversed caspase-3 and its fusion protein with part of the translocation domain of Pseudomonas exotoxin A. Compared with the control group, the expression of both genes caused in a similar degree the decrease of living cell numbers and massive occurrence of apoptosis.

结果成功获得了caspase-3基因，构建了重构型caspase-3基因和重构型caspase-3与绿脓杆菌外毒素转膜结构域部分肽段的融合蛋白基因及其表达载体，与对照组相比，在转染后1～4 d内，两种基因的表达均导致HeLa细胞存活数减少，大量细胞发生凋亡，且二者活性相当。

On the other hand, it is expressible as a Boolean expression if you accept including function calls in the Boolean expression.

但换个角度，如果你允许在布尔表达式中植入包含函数调用，则它就是一个布尔表达式可表述的属性。

If more than one operand of an expression raises an error, the implementation may choose which error is raised by the expression.

如果一个表达式有一个以上的操作数引发错误，实现可以选择哪一个错误被该表达式引发。

Making gfp fusion protein is a convenient method to mark the expression of interesting proteins, but it may interfere the expression of interesting proteins, since gfp is a quite big protein itself.

一方面，作为标记与目的蛋白融合可以方便的标示目的蛋白的表达情况，但gfp本身是个相当大的蛋白，会降低目的蛋白的表达；另一方面，进一步分离时需使用抗gfp的单克隆抗体进行免疫共沉淀，既不经济又不方便。

Above all, we take color part mediumly to do a specification to the graph, the small rectangle that brings facial expression is representing the communication port that can be sent and receives broadcast data package, have ' S ' the expression of the mark this port basically sends broadcast data package outwards, have ' R ' state this port receives broadcast data package only.

首先，我们对图一中的带颜色部分做一个说明，带颜色的小矩形都代表着能发送和接收广播数据包的通讯端口，有'S'标志的表示该端口主要向外发送广播数据包，有'R'表示该端口只接收广播数据包。

Objective: The purpose of our studies are gaining a better insight into the effect of genistein on mesangial cell"s phenotype change and ECM expression plus degradation under DN circumstances.We add genistein with different concentrations in high glucose cultured rat mesangial cells;feed DM rats with the single concentration genistein for different days;to explore the expression of SMemb,the mark of mesangial cell"s hypertrophy phenotype change;to examine the synthesis of Fn as well as the change of the balance between MMP-2 and TIMP-l;to comprehend its effect on renal pathological changes of DM rats and some related factors;trying toexplore the possible mechanisms.Through these studies,we will have some information about the experimental value of genistein in DN field.

目的：为了研究金雀异黄素对DN环境下大鼠MC肥大表型转换标志物SMemb和ECM分泌及降解的影响，我们拟在细胞水平以不同浓度的金雀异黄素作用于高糖培养下大鼠MC，在动物水平以同一浓度的金雀异黄素作用于DM大鼠不同时间，观察细胞表型转换标志物SMemb、ECM主要成分Fn、ECM降解酶系统基质金属蛋白酶—2(MMP-2)／基质金属蛋白酶抑制剂—1(TIMP-1)表达的变化，同时观察金雀异黄素对DM大鼠肾脏病理及影响肾脏病进展的糖脂代谢紊乱的影响，并进一步探讨金雀异黄素发挥作用的可能机制；为明确金雀异黄素在DN治疗中的地位及意义提供实验依据。

Three RNAi target sites (named as Abil1, Abil2, Abil3) targeting the E3B1 (NCBI: NM-024397), an identified target sites and positive control GAPDH-A were selected. The pGenesil-1 eukaryotic expression vectors with the neoR mark and GFP green fluorescent mark were selected to construct E3B1 RNAi plasmid. The effect of RNAi targeting various sites on E3B1 genes expression was evaluated by testing the transfection efficiency with fluorescence microscope and western blot. The most effective siRNA in inhibiting E3B1 genes were screened. The most effective siRNA in inhibiting E3B1 genes screened and used to transfect the neuronal cells. After that, the inhibitor of axon growth was added and the axon growth was observed. Result: The siRNA expressing plasmids targeting the E3B1 were constructed successfully.

选择针对E3B1(NCBI： NM-024397)RNAi的3个靶位点Abil1、Abil2、Abil3和1个不针对任何mRNA的RNAi靶位点以及甘油醛－3-磷酸脱氢酶，用带有neoR选择标志和GFP绿色荧光标志的真核表达载体pGenesil-1构建E3B1 RNAi质粒，分别转染培养的神经元，在荧光显微镜下观测转染率，经G418筛选得到单一的转染细胞，并用Western blot法检测各转染组神经元E3B1蛋白的表达情况，选出具有最佳抑制效应的siRNA转染神经元，加入轴突生长抑制物，观测轴突生长情况。

The expression level of cAPN gene mRNA in lung,glandular stomach and jejunum were higher than that in other tissues,and the expression level of it in bursa of Fabricius and spleen were lower than that in other.The results also reflected that the tropism of IBV is closely related to the cAPN content diversity.

表明cAPN的表达与IBV亲嗜组织存在着密切的相关性，同时也侧面反映了IBV组织嗜性的差异在一定程度上取决于机体不同组织器官cAPN的含量差异。

The expression level of cAPN mRNA in lung,glandular stomach and jejunum were higher than that in other tissues,and the expression level of it in bursa of Fabricius and spleen were lower than that in other.

结果显示，随着日龄增加，肝、脾、法氏囊、回肠、肾等组织中的cAPN表达量相对稳定，肺、腺胃、空肠等组织中的cAPN表达量呈现持续下降趋势，而十二指肠中的cAPN表达量持续上升，但盲肠、空肠中的cAPN表达量规律性不强且波动较大。

Analysis for testability is widely used in DFT and test pattern generation, but most testability analysis algorithms have the shortcoming of either inaccuracy or being too complicated. A new method to calculate the signal probabilities of the nodes in IC is pre-sented in Chapter 7 of this dissertation. The signal probabilities by our method is more exact than by the previous ones. A symbol of the signal probability at the fan-out node is created and is propagated to the RFON (Reconvergent Fan-out Nodes). At the RFON, the expression containing the symbol is simplified using the rule which corresponds to the law of identity in Boolean algebra. After the expression is simplified, the symbol is substituted with its numeric value. Theoretical analysis shows that this method is concise and has a low computing complexity.

可测试性分析在可测试性设计和测试生成中都有着广泛的应用，然而大部分的可测性分析方法都存在不精确或者过于复杂的毛病，在第七章中作者提出了一种新的计算IC电路节点的信号概率值的符号算法，该算法得出的信号概率值比用以往的算法法得出的要更准确，也更简洁，针对电路中的扇出节点重汇聚现象，我们在每一个扇出节点定义了一个信号概率的符号，在以后的计算中一直使用该符号参与计算，直到该符号传播至重汇聚处，利用一定的规则把含符号的表达式化简，再将符号的真实值代入，在计算符号概率的过程中定义了一个和布尔代数中的同一律相对应的运算规则，通过该运算规则，使得信号概率的结果更加准确，符号算法具有简洁方便，计算复杂度小的特点 4。

For the head-teacher of the class said that I had seriously broken the school rules,which led me to a three-day suspend class.

为什么呢？因为我的班主任说我严重的违反了校规，于是让我停课三天。

Many of them believe that the conversion of thousands of working-class folk in England spared that nation from the mass carnage and the tyranny that came with the revolution in France.

他们之中有许多人相信，在英国数以千计的劳工阶级之悔改信主使英国免於遭受如法国大革命所造成的大屠杀和专制暴政。