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Physics concepts, theorems as well as principles are primary content of physics learning, those knowledge take on the forms by proposition . In chapter 3, the study revealed that physics proposition are mainly obtained by reasoning (inductive reasoning such as tallying method, differentiation method and covariant method, deductive reasoning such as negative expression of hypothetical inference, affirmative expression of hypothetical inference ).Then by the means of experiment, the different condition needed when applying those reasoning was explored.

由于定义性概念、定理和定律等命题知识是物理教学的主要内容，所以在第三章中首先通过研究，表明物理命题主要是运用推理策略获得的(归纳推理策略中的求同策略、差异策略和共变策略，演绎推理策略)；然后通过实验等方法研究使用策略需要的条件；最后依据前面的研究提出适合物理命题学习的任务分析方法。

Model group and the normal group were taken distilled water,rats in the Tangshukang group were taken Tanggukang powder, western medicine control group were taken Calcium Carbonateand alpha D3. once a day for 12 weeks. Ca,P, BALP,BGP in blood and bone miner density;bone mineral contents, osseous vitodynamics,the content of Ca and P in the cremains were tested respectively.The femur was taken and detected the expression of TGF-β_1imRNA , the duodenum was taken and detected expression of VDR mRNA and CaBp-D9K mRNA with situ hybridization.

分别给予糖疏康、碳酸钙阿法迪三和生理盐水灌胃给药。12周后取材，测体重、血糖、血清钙、磷、血清骨源性碱性磷酸酶、骨T-羧基谷氨酸蛋白、骨密度、骨矿含量、骨的生物力学、骨干重、灰重、骨钙、磷等指标、组织病理切片骨形态计量学指标测定、并进行维生素D受体mRNA、转移生长因子-β_1(TGF-β_1)mRNA、钙结合蛋白(CaBp-D9K)mRNA原位杂交。

There was BMP4 expression at E10 in epithelium of otic capsule. Later, BMP4 expressions were affluent in condensed mesenchyme, vestibular cartilage capsule and sense epithelium. 3. The expression pattern of Smad4 in vestibule is different from BMP4. It was not conspicuous until E15 that Smad4 expressed in macula and crista ampullaris and until E16 in periotic cartilage capsule.

BMP4从E10开始，在间充质浓聚前就有表达；间充质一开始浓聚，BMP4在其中就有较丰富的表达；早期主要表达在前庭囊及前庭囊周围的间充质，在胚胎发育的后期，其在软骨囊及前庭上皮里有广泛阳性表达。3。

The "Experiment" generation of artists broke through the traditional aesthetic value system of the ink painting, and their exploration to rebuild the way of ink painting language through borrowing modern artistic concepts highlighted a cultural criticism, while the next generation of creators have vaguely presented a value orientation rather alien to that of the "Experiment" generation in their expression of contemporary emotions, experience and cognition as well as their construction of sensitivity and criticalness of contemporary cultural expression through the language of ink painting.

如果说"实验"一代艺术家们打破了水墨的传统审美价值体系，他们借鉴现代艺术观念重建水墨话语方式的探索突显出一种文化批判精神的话，那么，当下一些创作者在水墨语言对当代情感、经验、认知的表现力，对当代文化表达的敏锐性和批判性等方面的建构，则隐约显现出了与"实验"一代相异的价值取向。

Both chrysotile and crocidolite at given dose could stimulate type Ⅰ and type Ⅲ collagen gene expression by 2ES cell, suggesting that asbestos fibre itself may also contribute to collagen gene expression in asbestotic lung 〓.

一定剂量的温石棉和青石棉都可以刺激2BS细胞Ⅰ、Ⅲ型胶原基因的表达，表明石棉纤维本身也是引起石棉肺病变中胶原基因表达增加的重要原因。

The result show in transplanted vessel EF-1 and PCNA are mainly expressed in smooth muscle cells and Clemons rate proliferation of SMC is the core of endothelium proliferation.In the third part of our experiment we use fluid nitrogen to fire transplanted rein, crofter tissue homogeneous to test the expression of CE and EF-1 gene we use RT-Craned immunohistologiccal method to test mKNA and protein level respectively and analyze the expression of these tm genes in transplanted vein.

本实验的第二部分，我们将术后大鼠分为10组，每组8只，分别于术后6hours、24hours、72hours、1W、10days、2W、3W、4W、6W、8W切取移植静脉，将切取的不同时期的移植静脉分别置于10％中性福尔马林溶液中固定和液氮冷冻固定，将福尔马林溶液固定的标本制成4μm厚石腊切片，，常规进行病理观察大体改变，静脉标本HE染色后于光镜下观内膜增殖情况，并应用PCNA单克隆抗体，观察SMC增殖情况。

Results When Raji and B95-8 cells were cultured with 20-80 μg/ml of "Dongxia wan", the expression of EBV early antigen and EBV capsid antigen induced by croton oil and n-butyric acid and the natural expression of EBV-VCA were all inhibited significantly. The inhibition rates were 19.3%-49.84%, 34.63%-45.61% and 21.67%-47.78% respectively.

结果 20～80 μg/ml中药复方"冬夏丸"与靶细胞共培养能显著的抑制巴豆油和正丁酸联合诱发Raji细胞的EBV早期抗原和B95-8细胞的EBV壳抗原表达及B95-8细胞的EBV-VCA自然表达，抑制率分别是19.31%～49.84%，34.63%～45.61%和21.67%～47.78%。

To investigate whether the expression of cdc2 and cyclin B1 in spermatogenic cells during spermatogenesis is actually a temperature dependent event, in situ hybridization, Western blotting and immunohistochemistry analysis were used to study the expression of cdc2 and cyclin B1 in normal and cryptorchid testis. Results showed that heat would differentially hurt male germ cells in different developmental stages during spermatogenesis, especially the pachytene primary spermatocytes. Most of spermatogonia in contralateral cryptorchid testis were not harmed fatally by heat as yet, indicating that spermatogonia could resist to beat to a certain extent. In this case spermatogonia could develop to pachytene/diplotene primary spermatocytes, but they could not acquire the ability to complete the transition from mitosis to meiosis, and then appeared to go through apoptosis. Therefore, we could not find the descendants of meiosis: secondary spermatocytes and round spermatids, elongated spermatids and spermatozoon. The abdominal temperature had no significant influence on the transcription of cdc2 and cyclin B1 in the spermatogonia and pachytene/diplotene primary spermatocytes. In normal rabbit testis, cyclin B1 increased in the pachytene/diplotene primary spermatocytes before meiosis and reached its peak in the spermatids.

为了解精子正常发生过程中cdc2和cyclin B1表达的低温依赖性，我们利用原位杂交和免疫组化等方法，研究了正常和隐睾精子发生过程中cdc2和cyclin B1的转录和翻译调控活动，结果表明：（1）热对各阶段的雄性生殖细胞都有损害，粗线期的初级精母细胞尤为敏感，实验性隐睾内的精原细胞尚未完全受到"致命"影响，说明精原细胞对热有一定的耐受性，但即使成为粗线期／双线期初级精母细胞，却未能获得由有丝分裂过渡到减数分裂的能力，呈现不同程度的凋亡，所以在整个切片中找不到源自减数分裂的产物----次级精母细胞、圆形精子细胞，更谈不上长形精子细胞和精子的形成；（2）腹腔高温未明显地影响隐睾精原细胞和粗线期/双线期初级精母细胞中cyclinB1和cdc2的转录，说明高温并不是通过影响cyclin B1和cdc2的转录活动而导致生精过程阻断的；（3）正常兔睾丸组织中，〓在精原细胞和粗线期／双线期精母细胞中均有表达：cyclin B1蛋白在减数分裂前期的粗线期／双线期初级精母细胞中的表达量增加，于变态末期的精子细胞中达高峰。

The expression plasmid pGEX-5X-1/DFF45 was constructed and transformed into E.coli BL21, and the expression of GST-DFF45 protein was induced by IPTG. After purification, the fusion protein GST-DFF45 was used to immunize Oryctolagus cuniculus to obtain the antibody serum.

构建pGEX-5X-1/DFF45原核表达质粒，转化大肠杆菌BL21，用IPTG诱导融合蛋白表达，经纯化后免疫日本大耳白兔得到多克隆抗体并用CNBr-activated Sepharose？

Based on the definitions of metric tensor and curvature tensor, the full expressions of their changes are put forward with asymptotic analysis, where the metric tensor change expression coincides with Ciarlet's, and the curvature tensor change expression is described more accurately than Ciarlet's.

提出的度量张量的改变量与Ciarlet给出的一致，而曲率张量的改变量比Ciarlet给出的更精确。

"The operators will suffer a temporary decrease in the number of consumers and short message services as people may not like having to provide their ID cards for a phone number," Xu Junqi, vice-dean with the Policy-Making Institute of the Telecommunications Research Centre under MII, said yesterday.

信息产业部下属的通信决策研究院副主任徐俊其昨天说：&实行手机实名制后，人们可能不太情愿用身份证注册手机号，所以通信运营商会暂时面临用户减少和短信用户减少的问题。&

I don't believe in make-believe!

我不相信这些虚构的故事。