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The results of its intrinsic fluorescence spectroscopy and fluorescence phase diagram showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed a partially folded intermediate of Bacillus amyloliquefaciens a-amylase during its unfolding procedure, which followed a three-state model; the result of its fluorescence probe showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed some stable hydrophobic regions, which could interact with a hydrophobic reagent 8-anilino-1-naphthalene sulfonic acid, in the partially folded intermediate of Bacillus amyloliquefaciens a-amylase; and the results of fluorescence quenching using acrylamide and potassium iodide as quenchers showed the distribution of Trp residues in Bacillus amyloliquefaciens a-amylase in different denaturation solution, with the maximum number (8) of tryptophan residues in a partially folded intermediate Bacillus amyloliquefaciens a-amylase molecule could be quenched by potassium iodide; and the results of their protein electrophoresis and SEC showed that no aggregate or aggregate precipitation of Bacillus amyloliquefaciens a-amylase formed during the whole unfolding procedure of Bacillus amyloliquefaciens a-amylase induced by guanidine hydrochloride.

内源荧光光谱和荧光相图结果表明，当变性液中盐酸胍浓度约为1.0 mol/L时，芽孢杆菌a-淀粉酶的去折叠过程中出现一个部分折叠中间体，其去折叠过程符合&三态模型&；荧光探针结果表明，在溶液中盐酸胍浓度约为1.0 mol/L时，中间态芽孢杆菌a-淀粉酶分子中存在着能够与探针分子1-苯胺基-8-萘磺酸结合的稳定的疏水区域；荧光猝灭研究给出了不同程度变性的淀粉液化芽孢杆菌a-淀粉酶中的Trp的分布情况，结果表明中间态芽孢杆菌a-淀粉酶分子中能够被碘化钾猝灭的位于分子表面的色氨酸残基数目达到最大的8个；蛋白电泳和体积排阻色谱结果表明，在盐酸胍诱导的芽孢杆菌a-淀粉酶分子的整个去折叠过程中，不会以共价键或非共价键形式形成芽孢杆菌a-淀粉酶分子之间的集聚体或集聚体沉淀。

If it be shown in a reasonable number of well-authenticated cases that these conditions — exogamy as tribal law, and hostility as the prevailing relation of separate tribes towards each other — exist or have existed, accompanied, as might have been expected, by a system of capturing wives, we shall be justified in concluding — failing the appearance of any phenomena inconsistent with such an explanation — that the same conditions have existed in every case where the system of capture prevailed, or where the form of capture has been observed as a ceremony of marriage. Nothing more than this is necessary to satisfy the conditions of a sound hypothesis.We are in a position to do this and more.

并且如果它被证明在一个合理数目的被充分鉴别的案例中这些条件（异族结婚为部落法律，以及对抗是分离的部落相互对待的主要关系）存在或曾经存在，伴随着似可预期的一种猎妻制度，我们将被证明在做出结论时是正确的（舍弃与这样一种假设不协调的现象的出现）这些相同条件存在于流行劫掠制度、或者劫掠形式在婚礼中被观察到的地方的每一个案例。

Conclusions(1) Because of different types and magnitudes of LVH, LV function was significant impaired and LV motion asynchrony in systole and diastole existed in HCM patients; Impairment of LV function and synchrony existed only in diastole in HHD patients.

结论1。由于左室肥厚的发病机制、类型及程度不同，HCM患者存在严重左室心肌收缩、舒张功能下降，且左室运动于收缩期、舒张期均存在明显协调障碍；HHD仅于舒张期出现左室心肌功能下降及运动的不协调性。2。

The results showed that (1) COX-2 mRNA and protein existed in the mature male rat testis. COX-2 was localized in spermatocytes by immunohistochemistry.(2) COX-2 also existed in adult man testis. COX-2 protein was positively stained in spermatocytes and Leydig cells.(3) 2 weeks after administration of rofecoxib, the level of testosterone in the whole testis reached its lower values, being only 50% of control values. However, testosterone level recovered during 4 weeks. After such treatment, histologic examination of these testes showed atrophy of the seminiferous tubules and maturation arrest of spermatogenesis.(4) 2 weeks post-EDS, expression of COX-2 decreased significantly (P.005), in comparison with vehicle-treatment control. 4 weeks after treatment, a new generation of fetal like Leydig cells repopulated in the testicular interstitium resulting in COX-2 expression partially recovered. Although the expression of COX-2 mRNA and protein are enhanced at 8th week after using external testosterone, it wasn't significant higher than control group.

实验研究证明：(1)正常成年雄性大鼠睾丸组织中COX-2在mRNA和蛋白质水平均存在表达，免疫组织化学结果显示COX-2定位于曲细精管内的生精细胞；正常成年男性睾丸组织中同样存在COX-2表达，免疫组织化学结果显示COX-2定位于曲细精管内的生精细胞和Leydig细胞；(2)服用特异性COX-2酶抑制剂rofecoxib 2周后，实验组大鼠睾丸组织内睾酮的含量减少，为正常对照组的50％；持续用药4周后睾丸组织内睾酮浓度逐渐恢复至正常水平；COX-2酶活性降低后病理组织切片显示睾丸内曲细精管萎缩，生精紊乱，持续用药4周时影响最明显；(3)注射特异性Leydig细胞杀灭剂EDS 2周后，实验组大鼠睾丸组织内睾酮浓度降至极低水平时，COX-2的蛋白和基因表达水平也显著低于正常空白对照组（P.005）；使用EDS后第4周，睾丸组织中的睾酮浓度逐渐回升，同样组织中COX-2蛋白表达和mRNA表达水平也相应提高接近正常水平；使用EDS后4周开始给予外源性睾酮，6周和8周时COX-2表达水平的绝对值虽有提高，但与正常大鼠空白对照组比较并无显著性差异，说明外源性雄激素刺激睾丸合成COX-2的作用并不明显。

The significant correlation existed in the total litter, number of ablactation pigs and litter weight after ablactation.

其中DD和BB基因型的个体上述生产性能显著高于BB和AA基因型的个体，也高于BD和AB基因型的个体，但差异不显著。

The French had shewn themselves the ablest architects of ruin that had hitherto existed in the world.

法国人已经表明了他们是世界迄今为止的最能干的破坏建筑师。

In Isaac Newton's view, space was absolute - in the sense that it existed permanently and independently of whether there were any matter in the space.

在艾萨克牛顿认为，空间是绝对的-在这个意义上讲，它的存在和独立的永久是否有任何问题的空间。

As a social institution, academic science has existed for more than one century.

学院科学作为一种社会建制化的学术活动，至少在高等教育中存在了一个多世纪。

HLG of the 2nd grade acquired higher accuracy than LLG, and no significant difference was found either between HLG and MLG or between MLG and LLG; HLG of the 3rd grade had higher accuragy than both MLG and LLG, and no significant difference existed between MLG and LLG.

其中，在准确性方面，2年级高组高于低组，而高组与中组、中组与低组间均无显著差异，3年级的高组高于中组和低组，而中组与低组间无显著性差异。

The immunoregulation activity of SPG-1 mainly existed in the part of sugar chain, but keeping its molecular structure intact had some effects on its immunoregulation activity; SPG sulphation had reducing effects on the immunoregulation of SPG-1, whereas, after medium and low acetification of SPG, the immunoregulation of SPG was enhanced. Conversely after high acetification, the immunoregulation of SPG-1 decreased, that is, after introducing sulfuric and acetic acid base in SPG-1, its immunoregulation changed. All of these indicated the structure of SPG-1 was closely connected with its immune activity.⑤.

甘薯糖蛋白SPG-1的免疫调节活性主要在其糖链部分，但保持分子结构的完整性对其免疫调节的活性有一定的作用；甘薯糖蛋白硫酸化对甘薯糖蛋白SPG-1的免疫调节有降低作用，而中、低度乙酰化后，可提高甘薯糖蛋白的免疫调节作用，高度乙酰化后则降低其免疫调节作用，即甘薯糖蛋白引入乙酰基和硫酸基团后，其免疫调节活性发生了改变，这些均表明了甘薯糖蛋白的结构与其免疫调节活性有密切的关系。

The second is to cut a mere 100 calories from your diet , which is the equivalent of a tablespoon of butter.

第二个就是从你的饮食中减少一百卡路里，只是相当于一大汤匙的黄油。

This is where the Rorschach test comes in.

现在，罗氏测试就出现了。