查询词典 elicitor

Keywords Cell culture;Plant secondary metabolism;Elicitor;Hairy-root;Plant genetic engineering

细胞培养；植物次生代谢；诱导子；毛状根；基因工程

Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合，而且结合是饱和性的、可被竞争的，符合配体-受体结合的典型特征，同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性，表明水稻细胞质膜上存在nsLTP的受体；利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜，再进行结合实验，结合活性几乎不受影响；而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失，表明其受体为没有经过糖基化修饰的蛋白质；利用交联剂BS~3交联配体一受体后，再进行SDS-PAGE分离和放射自显影，结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合，可能是其受体；细胞外碱化实验表明，nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应，却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应，表明nsLTP和Cryptogein结合细胞膜上相同的位点，保护了植物细胞免受Cryptogein导致的细胞程序性死亡，并诱导系统获得性抗性的产生；体外Pull-down实验表明，nsLTP144和水稻的OsCaM1具有相互作用，暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

In addition, in suspension cells, expression of both GaWRKY1 and CAD1-A genes and biosynthesis of sesquiterpene aldehydes were strongly induced by a fungal elicitor-preparation and methyl-jasmonate .

另外，在亚洲棉悬浮细胞中，GaWRKY1和CAD1-A的转录水平均倍半萜醛类物质的合成均可以被黄萎病菌激发子和MJ诱导。

We took the cultured suspension cells ofPanax ginseng as our experimental material, studied the generation of singlet oxygen whichinduced by fugal elicitor, testified the singlet oxygen acted as a signal molecule in plantdefense responses.

我们以人参悬浮细胞为实验材料，研究了真菌激发子诱导下单线氧的产生情况，进一步证明了单线氧在植物防卫反应中作为信号分子的作用。

Objective To compare influence of several biotic elicitors （yeast extract, oligogalacturonides, and fungal elicitor） and abiotic elicitors （Ag^＋, Co^2＋, and a-amino isobutyric acid） on tanshinone production in the hairy root culture of Salvia miltiorrhiza.

目的比较几种生物诱导子酵母提取物、寡半乳糖醛酸、真菌诱导子3和非生物诱导子（Ag^＋、Co^2＋和α-氨基异丁酸）对丹参毛状根培养生产丹参酮的影响。

B-glucosidase the elicitor collected from Pieris brassiae larva oral secretions , which incur indirect defense of cabbage plant to P.

用已明确的大菜粉蝶口腔分泌物诱导因子b-葡萄糖苷酶（b-glucosidase）处理小麦幼苗，经鉴定、分析，该酶没有引起小麦的间接防卫反应。

HT-toxin, which produced by Exserohilum turcicum, is not only a major toxigenic factor but also the elicitor to stimulate the defensive reaction of corn.

对HT-毒素的研究，将有助于研究玉米大斑病菌引发玉米抗病/感病的分子机制。

The results showed that, the oligosaccharides have elicitor activity, and could induce the notable increase of PAL, chitinase,β-1, 3-glucanase activity and accumulation of hydroxyproline-rich glucoprotein and lignin.

结果表明，该低聚糖具有明显的激发子活性，能够诱导杨树细胞苯丙氨酸解氨酶、几丁质酶、β-1，3-葡聚糖酶活性显著增高（分别为对照的2.80，1.99，2.84倍）和木质素、HRGP的积累（分别为对照的2.35，2.61倍）。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。