查询词典 effect binding

The primary conclusions of the present work are as following first, the charges of membrane sarface effect the rate of protein adsorption, but do not effect the amount of protein ultimately adsorbed second, the nonspecific adsorption of avidin can be detached by adding a suitable amount of bivalent alkaline-earth metal ions; third, the binding of avidin to membrane bound model receptor is astricted remarkable by the latelal steric hindrence.

实验结果表明，膜表面电荷对非特异性吸附的速率有很大的影响，而对最终的蛋白吸附量影响不大；非特异性的吸附可以通过二阶阳离子的脱附而去除；受体蛋白质与配体间的特异性结合受到侧向空间位阻效应的制约。

Effect of amphetamine and phencyclidine on DNA binding activities of serum response and dyad symmetry elements .

影响安非他明和苯环的DNA结合活动的血清反应和对子对称要素。

Theresults suggested that they both intercalate into DNA as well as that the different positions of substituent groups don\'t effect on the binding mode but effect on thebinding affinity.

文中还研究了这两个配体对HeLa、HepG2以及L02的药理活性，结果表明它们对肿瘤细胞HeLa、HepG2有较强的抑制能力，对正常细胞L02的抑制效果较差。

The SEM-EDS showed that the content of surface elements was changed after treated with glutaraldehyde or albumin indicated that argon plasma only had surface cleaning effect and did not change surface composition and the allylamine deposition thickness was too thin to show the difference in SEM-EDS findings. The XPS results confirmed the argon plasma cleaning effect and revealed that the ratio and the binding energy of surface elements changed with different treatment procedures and were similar to what expected to coat on the titanium surface.

XPS更进一步证实，氩气电浆只有清洁钛金属表面的作用，并不会改变其表面化学组成；其余各组的钛、碳、氮、氧的比例及键结能量随著处理程序而改变，可知表面上有化学组成之改变，且随不同处理程序所得到表面元素之百分比及键结能量，与预期的表面披覆物元素百分比及键结能量相近。

Since it has many special mechanical,optical, electrical, magnetic, calorifics and chemical properties which differ from thecorresponding bulk materials due to its size effect, surface effect and quantum effect,as a wide band gap semiconductor material with large exiton binding energy of 60meV at room temperature, it is attractive for many new applications in industry andhigh-tech fields.

由于ZnO是一种直接带隙的半导体材料，室温下能隙宽度为3.37 eV，激子束缚能高达60 meV，具有优良的物理和化学性质，其显著的表面效应、量子尺寸效应和宏观量子隧道效应，是目前光电子研究领域的热点。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

Previous studies by others and this laboratory on the allosteric effect of hem oglobins have suggested that the electrostatic replusive cavity at the center of tetrameric moiety plays a substantial role in regulating O2 transport prope rties, including chloride binding, and Bohr effect .

中文摘要过去对血红蛋白异位调节效应的研究指出，在血红蛋白四合体中央空腔的区域的静电排斥效应对分子机能的调节如氧合时氯离子的键结和波尔效应，扮演著一个很重要的角色。

However, fully consideration of special structure and physiological function of CRP is critical to investigate whether CRP provide protect effect or pathogenic effect in the progression of atherosclerosis. CRP is a member of pentrxins family, which consists of five identical, noncovalently associated 23-kDa protomers arranged symmetrically around a central pore with a 102 A external diameter. Each protomer has a recognition face with a phosphocholine binding site consisting of two coordinated calcium ions adjacent to a hydrophobic pocket. The opposite face of the pentamer is the effector face, where complement Clq binds and Fc receptors are presumed to bind. A cleft extends from the center of the protomer to the central pore of the pentamer. Both faces ofpentamer form a special bi-phase structure: one face is the ligand recognition phase, which can recognize apoptosis cells and enzyme modified low density lipoprotein in which PCh is exposed.

CRP是一种五聚体蛋白，外径为102 A，由5个相同的单体以非共价键结合，形成双面的环形结构，每个单体的配体识别位点或受体结合位点分别位于五聚体两个平面上，分别组成配体识别相和效应器相，从而构成特殊的二相性结构：一面为配体识别相，含有磷酸胆碱(phosphorycholine，PCh)结合位点，能够使CRP识别在病理条件下暴露出PCh的凋亡细胞、酶修饰低密度脂蛋白(enzyme modified low density lipoprotein，E-LDL)等。E-LDL是天然低密度脂蛋白(native Low density lipoprotein，N-LDL)在多种蛋白酶，如胰蛋白酶、神经氨酸酶、胆固醇酯酶等作用下的代谢产物，其主要特点是结构发生改变(N-LDL大小均匀，平均直径250±30 A，E-LDL大小差异极大，直径为100-2000 A)，暴露磷酸胆碱位点，能够被CRP识别；另一面为效应器相，能够与巨噬细胞、单核细胞表面受体FcγRⅡa结合，介导巨噬细胞、单核细胞识别及吞噬凋亡、坏死细胞作用。

It's not that a binding site change can have no effect, but rather that it cannot have the required effect —the complete top-down reorganization needed for a transition to a substantially different form of life.

结合位点的变化并不是没有效果（它在具体情况下可能有重要的意义），而是达不到所要求的效果——产生完全不同的生命形态所要求的全面的生物结构重组。

In pelletization process, slaked lime, clinker and calcined gypsum can be used as additives of which the hydration products of clinker have a binding effect of gypsum grains and a seed crystal effect for cement hydration. Thus, this technology is one of the best pelletization technologies known so far.

以熟石灰、水泥熟料、熟石膏为成粒助剂的成粒工艺，将磷石膏中和预处理统一于成粒过程中，熟料水化产物既对石膏颗粒起固化作用，又对水泥水化产生晶种效应，是国内外最好的成粒工艺技术之一。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。