查询词典 effect binding

Peripheral plasma membrane proteins extracted from the spermatozoa from caput,corpus and cauda epididymis as well as vas deferens were probed with four lectins—Arachis hypogaea agglutinin、Canavalia ensiformis agglutinin、Dolichos biflorus agglutinin、Triticum vulgaris agglutinin.The results indicated that the 104 and 95kD AHA binding glycoproteins,and the 117、114、104、87、76、70kD ConA binding glycoproteins were synthesized and modified in the testis;the 120 and 65kD ConA binding glycoproteins,and the 54kD DBA binding glycoprotein were modified or lost during epididymal maturation;the 109kD ConA binding glycoprotein,the 74、70、45kD DBA binding glycoproteins,and the 57kD TVA binding glycoprotein were modified during epididymal maturation.

结果表明：与花生凝集素结合的104kD和95kD糖蛋白，与伴刀豆凝集素结合的117、114、104、87、76、70kD糖蛋白在睾丸中已完成了蛋白合成及修饰过程；与伴刀豆凝集素结合的120kD和65kD糖蛋白，与双花扁豆凝集素结合的54kD糖蛋白在精子附睾成熟过程中被修饰成其它种类的蛋白或被丢失；与伴刀豆凝集素结合的109kD糖蛋白，与双花扁豆凝集素结合的74、70、45kD糖蛋白，以及与麦胚凝集素结合的57kD糖蛋白是在附睾中完成其修饰过程的。

It was found that La〓 does not affect the binding affinity between calmodulin and Polistes Mastoparan, a known calmodulin binding peptide, neither the conformation of the ternary complex. Excessive amount of La〓 result in the decrease of the binding constant and the disrupted conformation. The binding affinity of La〓 to calmodulin increased in the ternary complex (La〓-CaM-Mas/Mas X), which suggested the coordination between the two global domains. The binding priority between the two global domains is also changed: La〓 more is likely to bind to the C-terminal of calmodulin than to N-terminal, thus facilitates the binding of Mas/Mas X to the C-terminal of calmodulin as the first step of the Mas/Mas X binding.

在以钙调蛋白结合肽—Polistes Mastoparan和Mastoparan X为对象的研究中，我们发现，除非过量，否则La〓的存在不影响钙调蛋白与Mas间的结合常数以及构象；过量的La〓（La〓/CaM摩尔比＞4）将引起钙调蛋白结合功能的下降，同时明显改变金属-钙调蛋白-Mas三元复合物的构象；La〓对Ca〓CaM-Mas的N末端表现高度选择性，显示了在三元复合物中两种离子间的协同效应；在三元体系中，La〓与钙调蛋白的亲和力明显上升，而且亲和力上升的程度因钙调蛋白结合肽的不同而有明显差异，显示了作用的选择性；Mas和MasX的存在改变了La〓在钙调蛋白上的结合顺序，La〓很可能优先与C末端结合，从而使Mas/Mas X首先与C末端结合，该顺序与钙离子相同；La〓的参与使三元复合物在动力学上更加稳定。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

The successive stability constants which are obtained by non-linear least-squares methord fitting Bjerrum formula. In three- system ,the results from Scatchard plots indicate that there exists two strong binding site of Mn and one strong binding site of Zn,there are same results compareing with two -system ,but some more weak binding sites are different from the different addition sequence ,show that there are not common strong binding site of Mn,Zn in HSA or BSA .Strong binding sites have electiveness and unfalteringness ,weak binding sites exists irreversibility.

用非线性最小二乘法拟合Bjerrum方程，计算了同时竞争体系的逐级稳定常数；Scatchard图分析表明，三元体系中，Mn和Zn与血清白蛋白结强合部位数分别为2和1，与二元体系相比，保持不变，弱结合部位数随加入顺序的不同而不同，但都有所增加，竞争结果说明了Mn和Zn与血清白蛋白结合没有共同的强结合部位，强结合部位具有专一性，选择性，而弱结合部位具有不可逆性。

Membrane affinity quantified by IAM and liposome/buffer systems was a more efficient predicator of α values than hydrophobicity from n-octanol/buffer system. Consequently, not only hydrophobic force but also attractive polar extra-interactions involved in the binding to IAM and liposomal membranes but not in an n-octanol phase, ascribed to the binding process within AM. The binding to ordered lipid membrane in the larger part determined drugs'binding to AM intracellular compositions, and the ordered phospholipid plasma membrane was a potential site for drugs'binding in AM.

脂质体／水系统和磷脂膜色谱所测定的膜亲和性比正辛醇／水系统的疏水性更好的预测巨噬细胞内药物的α值，因此包含在磷脂膜色谱中但并不体现在正辛醇／水系统中药物与磷脂膜之间的额外吸引性极性作用力对药物在细胞内结合有着重要贡献；药物与有序磷脂膜的结合在很大程度上决定着药物与巨噬细胞内成分的结合，有序磷脂分子膜可能是巨噬细胞内部潜在的结合部位。

Company Introduction: Ningbo Wenbai Machinery Manufacturing Co.,Ltd. specialize in manufacturing book binding machines. The products mainly cover three categories:1.loose-leaf binding machines : double wire forming machine, double wire binding machine, semi-automatic/ fully-automatic punching machine, spiral binding machine and related materials;2.perfect binding machines : various type paper-folding machines, gathering machines, three-knife trimmers, perfect binders, etc.3.corrugated box die-cutting machine: automatically plate-to-plate die-cutting machine,laminating machines, UV varnisher glazing machines, and overwrapping machines.

公司简介：宁波文百机械制造有限公司专业制造书刊装订类设备，产品主要分三大块：一是活页本装订系列产品，主要有双线圈成型机，双线圈装订机，半/全自动冲孔机，单线圈装订机及相关耗材；二是书刊装订系列设备，主要有各种折页机，配页机，三面切书机，椭圆胶订包本机等；三是包装系列主要有瓦楞模切类设备，主要有全自动平压平模切机，复膜机，UV上光及药品、食品包装产品。

We first studied the interaction mechanism of Erythrosin B with proteins by spectrophotometry and fluorospectrophotometry, calculated their binding number, binding constants and binding site, and estimated their binding type.

将分光光度法和荧光光度法结合，首次研究了四碘荧光素与蛋白质的作用机理，计算了结合数、结合常数和结合位置，判断出结合类型。

Are: Belgium Borg page with high-end machines, plastic installed series; Germany EBA cutter, shredder; German high Star drilling machine, binding machine; Japan Uchida page with machine , folding machine; Switzerland Opel upmarket folding machine; Sweden Reiter small binding machine series; USA wireless grams fastback binding machine; British KAS nail fold family of products, the Japanese love fat plate machine; Germany Reeling love spiral binding machine series; Japan trump card business cards, etc.

主要有：比利时『博格』高档配页机、胶装机系列；德国『EBA』切纸机、碎纸机；德国『斯达高』钻孔机、装订机；日本『内田』配页机、折页机；瑞士『欧宝』高档折页机；瑞典『瑞特』小型装订机系列；美国『快背克』无线装订机；英国『KAS』钉折系列产品，日本『爱发』制版机；德国『爱丝特』螺旋线装订机系列；日本『皇牌』名片机等数十个品牌的印刷、制版及装订系列设备。

Membership card after binding main equipment: two Muller Martini binding line of fnji xingguang, half the Coles leboss binding line, a purple light disc binding package in its native.

会员卡制作后胶订主要设备：两条马天尼无线胶订联动线富士星光，半条柯尔布斯无线胶订联动线，一台紫光圆盘胶订包本机。

The central issue of this paper is the study of DNA-binding proteins interacted with DNA replication origin. The paper presented here includes there parts which are related as well as independent of each other:(1). Identification and partially purification of DNA-binding proteins interacted with eukaryotic DNA replication origin;(2) Specific binding of Hela cell proteins to Samian Virus 40 replication origin with the cruciform structure (Stem-Loop structure);(3) The study of DNA-binding protein as a tumor marker.

本文对DNA结合蛋白的研究主要包括三个即有关联，又相对独立的方面：（1）猿猴肾脏细胞复制起始区DNA结合蛋白的鉴定和部分纯化；（2）Hela细胞中DNA结合蛋白对含茎环结构的SV40复制起始区DNA的特异性结合；（3）DNA结合蛋白做为肿瘤征兆物的研究。

A backer of an Afghan law that critics say legalizes marital rape has rejected the international outcry as foreign meddling.

饱受批评的一项法律的拥护者说合法化的婚内暴力不会受国际舆论的干涉。

Years ago, a ancient Egyptian fleet went to the Land of punt for seeking a kind of flavor named "myrrh", and aromatic plants which has dense exotic style for the queen Hatshepsut.

3500年前的一个古埃及的舰队到&彭特之地&寻找一种叫没药的香料。