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The effects and mechanism of GABAergic neurons, NOergic neurons, opioid peptide and cyclic adenosine monophosphate in the nucleus reticularis thalami on sleep-wakefulness cycle of rats and the effects and mechanism of the 5-HTergic nerve fibers project from the nucleus raphes dorsalis to RT on sleep-wakefulness cycle of rats were investigated with the methods of brain stereotaxic, nucleus spile, microinjection and polysomngraphy.1. The effects of GABAergic neurons in RT on sleep-wakefulness cycle of rats1.1 Microinjection of 3-mercaptopropionic acid (3-MP, a kind of glutamate decarboxylase inhibitor) into RT. On the day of microinjection, sleep only decreased a litter. On the second day, sleep marked decreased and wakefulness marked increased. On the third and fourth day, sleep and wakefulness stages resumed to normal.1.2 Microinjection of gamma-amino butyric acid (GABA 1.0μg) into RT enhanced sleep and reduced wakefulness compared with control; while microinjection of L-glutamate (L-Glu, 0.2μg) decreased sleep and increased wakefulness; microinjection of bicuculline (BIC, 1.0μg), a GABAA receptor antagonist, enhanced wakefulness and reduced sleep; microinjection of baclofen (BAC, 1.0μg), GABAB receptor agonist, had the same effects as GABA.2. The effects of NOergic neurons in RT on sleep-wakefulness cycle of rats2.1 Microinjection of L-arginine (L-Arg, 0.5μg) into RT decreased sleep compared with control, but there were on statistaical difference between L-Arg group and control; while microinjection of sodium nitroprusside (SNP, 0.2μg), a NO donor into RT, sleep marked decreased and wakefulness marked increased. Microinjection of nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NNA, 2.0μg) into RT enhanced sleep and reduced wakefulness.2.2 After simultaneous microinjection of L-NNA (2.0μg) and SNP (0.2μg) into RT, SNP abolished the sleep-promoting effect of L-NNA compared with L-NNA group; after simultaneous microinjection of L-NNA (2.0μg) and L-Arg(0.5μg) into RT, we found that L-NNA could not blocked the wakefulness-promoting effect of L-Arg.3. The effects of opioid peptide in RT on sleep-wakefulness cycle of rats3.1 Microinjection of morphine sulfate (MOR, 1.0μg) into RT increased wakefulness and decreased sleep compared with control; while microinjection of naloxone hydrochloride (NAL, 1.0μg), the antagonist of opiate receptors, into RT, enhanced sleep and reduced wakefulness.3.2 After simultaneous microinjection of MOR (1.0μg) and NAL (1.0μg) into RT, the wakefulness-promoting effect of MOR and the sleep-promoting effect of NAL were not observed compared with control.4. The effects of cAMP in RT on sleep-wakefulness cycle of rats Microinjection of cAMP (1.0μg) into RT increased sleep and decreased wakefulness compared with control; microinjection of methylene blue (MB,1.0μg) into RT enhanced sleep and reduced wakefulness compared with control.5. The effects of the 5-HTergic nerve fibers project from DRN to RT on sleep-wakefulness cycle of rats5.1 When L-Glu (0.2μg) was microinjected into DRN and normal sodium (NS,1.0μg) was microinjected into bilateral RT. We found that sleep was decreased and wakefulness was increased compared with control; when L-Glu (0.2μg) was microinjected into DRN and methysergide (MS,1.0μg), a non-selective 5-HT antagonist, was microinjected into bilateral RT, We found that sleep was enhanced and wakefulness was reduced compared with L-Glu group.5.2 When p-chlorophenylalanine (PCPA, 10μg) was microinjected into DRN and NS (1.0μg) was microinjected into bilateral RT, We found that sleep was increased and wakefulness was decreased compared with control; microinjection of 5-hydroxytryptaphan (5-HTP, 1.0μg), which can convert to 5-HT by the enzyme tryptophane hydroxylase and enhance 5-HT into bilateral RT, could block the effect of microinjection of PCPA into DRN on sleep-wakefulness cycle.

本研究采用脑立体定位、核团插管、微量注射、多导睡眠描记等方法，研究丘脑网状核(nucleus reticularis thalami，RT)中γ-氨基丁酸(gamma-amino butyric acid ，GABA)能神经元、一氧化氮(nitrogen monoxidum，NO)能神经元、阿片肽类神经递质、环一磷酸腺苷(cyclic adenosine monophosphate，cAMP)及中缝背核(nucleus raphes dorsalis，DRN)至RT的5-羟色胺(5-hydroxytryptamine，5-HT)能神经纤维投射对大鼠睡眠-觉醒周期的影响及其作用机制。1 RT内GABA能神经元对大鼠睡眠-觉醒周期的影响1.1大鼠RT内微量注射GABA合成关键酶抑制剂3-巯基丙酸(3-MP，5μg)，注射当天睡眠时间略有减少，第二日睡眠时间显著减少，觉醒时间明显增多，第三、四日睡眠和觉醒时间逐渐恢复至正常。1.2大鼠RT内微量注射GABA受体激动剂GABA( 1.0μg)后，与生理盐水组比较，睡眠时间增加，觉醒时间减少；而RT内微量注射L-谷氨酸(glutamic acid， L-Glu， 0.2μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAA受体阻断剂荷包牡丹碱(bicuculline，BIC，1.0μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAB受体激动剂氯苯氨丁酸(baclofen，BAC，1.0μg)后，产生了与GABA相似的促睡眠效果。2 RT内NO能神经元对大鼠睡眠-觉醒周期的影响2.1大鼠RT内微量注射NO的前体L-精氨酸(L-Arg，0.5μg)后，与生理盐水组对比，睡眠时间略有减少，但无显著性意义；而RT内微量注射NO的供体硝普钠(Sodium Nitroprusside，SNP，0.2μg)后可明显增加觉醒时间，缩短睡眠时间；微量注射一氧化氮合酶抑制剂L-硝基精氨酸(L-arginine，L-NNA，2.0μg)后，引起睡眠时间增多，觉醒时间减少。2.2大鼠RT内同时微量注射L-NNA(2.0μg)和SNP(0.2μg)后与L-NNA组比较发现SNP逆转了L-NNA的促睡眠作用；RT内同时微量注射L-NNA(2.0μg)和L-Arg(0.5μg)后，与L-NNA(2.0μg)组比较发现L-Arg可以增加觉醒而缩短睡眠，其促觉醒作用未能被NOS的抑制剂L-NNA所逆转。3 RT内阿片肽对大鼠睡眠-觉醒周期的影响3.1大鼠RT内微量注射硫酸吗啡(morphine sulfate，MOR，1.0μg)后与生理盐水组对比，睡眠时间减少而觉醒时间增加； RT内微量注射阿片肽受体拮抗剂盐酸纳洛酮(naloxone hydrochloride，NAL，1.0μg)后与生理盐水组比较，睡眠时间增加而觉醒时间减少。3.2大鼠RT内同时微量注射MOR(1.0μg)和NAL(1.0μg)后，与生理盐水组对比，原有的MOR促觉醒效果和NAL的促睡眠效果都没有表现。4 RT内环一磷酸腺苷信使对大鼠睡眠-觉醒周期的影响大鼠RT内微量注射cAMP(1.0μg)后与NS(1.0μg)组比较，睡眠时间增多而觉醒时间减少；RT内微量注射亚甲蓝(methylene blue，MB，1.0μg)后，与NS组比较，睡眠时间增多而觉醒时间减少。5中缝背核投射到丘脑网状核的5-羟色胺能神经纤维对大鼠睡眠-觉醒周期的影响5.1大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 0.2μg)比较，睡眠时间减少，觉醒时间增多；大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射二甲基麦角新碱(methysergide， MS， 1.0μg )后，与对照组(DRN注射L-Glu 0.2μg，双侧RT注射NS 1.0μg)比较，睡眠时间增多，觉醒时间减少。5.2大鼠DRN内微量注射对氯苯丙氨酸(p-chlorophenylalanine，PCPA，10μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 1.0μg)比较，睡眠时间增多，觉醒时间减少；大鼠DRN内微量注射PCPA(10μg)，产生睡眠增多效应后，在双侧RT内微量注射5-羟色胺酸(5-hydroxytryptaphan ， 5-HTP， 1.0μg )后，与对照组(DRN注射PCPA 10μg，双侧RT注射NS 1.0μg)比较，睡眠时间减少，觉醒时间增多。

If you say , you must stretch your lips back; for you must round your lips forward; for you drop your jaw down; for you will drop your jaw far down and back; for bring your lips back and drop your jaw a bit; for drop your jaw for the ah part of the sound and pull it back up for the ee part; and for round the lips, drop the jaw and pull back up into .

如果你说，你必须把嘴唇伸展回去；呢，你必须让自己的嘴唇变园；而对于，你需要把下颚放低；对你需要把你的下颚放低再复原；对于，把你的嘴唇收回去，并且稍微放低下颚；呢，就要在ah这个部分放低下颚然后在ee这个部分复原；对于嘴唇成圆形，放低下颚再拉回来发出这个音。

At least, we should memorize the use of the several phrases such as the five meanings of "pick up", drop off and its relevant drop in, drop sb. a line, drop behind, drop out, power used as verb, means and its phrases: by means of; by no means; by this means.

我们至少该记住几个短语的用法，如pick up所表达的五个含义：捡起；用车接……；获得，学到，得到；好转，改进；接收节目。drop构成的各种短语及含义：drop off 散去；走掉；下车；drop in 顺便来访， drop sb。

Point to successive two days appear steep fall, shanghai points to drop for a time approach 5000 bits of col, two city do much power to continue to drop, those who drop from Zhou San occupy about 7 Chengshangsheng arrives to be controlled most probably; And two city clinch a deal 267.2 billion yuan, continue to the limit of one's capacity 7 percent, point to appear to drop urgently continuously of to the limit of one's capacity bad go situation, have 21 drop more than 8%, part a brunt capital is more apparent from field evidence.

股指连续两天出现暴跌，沪指一度下跌逼近5000点关口，两市做多动力继续减退，下跌的个股从周三的约占七成上升到八成左右；而两市成交2672亿元，继续放量7个百分点，股指呈现连续急跌放量的不妙走势，有21只个股跌幅超过8%，部分个股主力资金离场迹象比较明显。

The pressure drop of the compound tray can be regarded as consisting of dry plate pressure drop, clear liquid resistance, liquid surface tension resistance, and packing pressure drop. The model that has established can fairly predict the pressure drop of the compound tray, the value calculated by the model compared with that detected by experiment is less than 20%.

复合塔板的压降可认为由干板压降、清液层阻力、填料层压降和克服液体表面张力的压降四部分组成，所建立的压降计算模型，能较好地预测复合塔板的压降，计算值与实验值误差在±20％以内，可用于工程设计。

The 3rd person sleeps the eye cheats 眬 ground to rise station outside piddle, domestic wine lane is next door, the drop of drop of acoustical drop drop that extracts wine is ceaseless, he thinks he passes water unfinished, stand to dawn all the time unexpectedly.

第三个人睡眼矇眬地起来站在外面撒尿，隔壁是家酒坊，榨酒之声滴滴沥沥不停，他以为自己小解未完，竟一直站到天明。

In order to reduce the energy of that part, three kinds of pressure drop reducing structures are devised and experimented together, such as the straight stick pressure drop reduction frame, grid pressure drop reduction frame and the taper pressure drop reduction frame.

本文设计并实验了三种不同类型的减阻结构：直杆减阻框、栅格减阻框和锥形减阻框以降低排气管内的流动阻力损失。

The results of pressure drop characteristics experiments indicate that the new type cyclone tube's pressure drop reduction extent is directly related to the sticks'number, length and the radial position of the straight stick pressure drop reduction frame; is directly related to the length, width and number of grid pressure drop reduction frame installed under the split flow core tube.

安装在排气芯管内的直杆减阻框、栅格减阻框使旋风管的压降有不同程度的降低，减阻幅度与直杆减阻框杆数和长度成正比；与直杆的径向位置大小成反比；与安装位置、绕流截面形状有一定的关系；并与栅格减阻框的长度、宽度和片数成反比。

Applied with test of potted plant and measurement absorbency for study response of the contents of photosynthetic pigments of slash pine's half-sib under water stress. There are 7 families comparison with general slash pine. The contents of photosynthetic pigments are mensurated under feebleness water stress while soil water content is 55% to 60% and moderate water stress while soil water content is 35% to 40% and heavy water stress while soil water content is 20% to 25%. The test result indicate that either water grads or families of chlorophyll and chlorophyll a and chlorophyll b and Chla/Chlb and carotenoid and Caro/Chl are mighty notable difference. The Chl and Chla and Chlb and Chla/Chlb little drop from normal soil water to initial water stress. Then drop obvious with increased water stress. The Caro and Caro/Chl little drop firstly from normal soil water to initial water stress,then up in metaphase water stress,after drop in evening water stress. The Chl and Chla and Chlb and Chla/Chlb and Caro of slash pine'half-sib are over general slash pine. The Chl and Chla and Chlb and Chla/Chlb and Caro of 464 and 1027 are over average value of slash pine's half-sib under water stress. The Caro/Chl of 609 and 46 are over average value of slash pine's half-sib under water stress.

为研究湿地松半同胞家系光合色素对水分逆境的响应，采用盆栽试验和吸光度测定方法，以湿地松普通种子为对照，测定了七个湿地松优良半同胞家系在弱度水分胁迫（土壤相对含水量为55%~60%）、中度水分胁迫（土壤相对含水量为35%~40%）和强度水分胁迫（土壤相对含水量为20%~25%）条件下的光合色素，对湿地松优良半同胞家系在不同水分胁迫条件下的光合色素进行了研究，结果表明：无论是不同水分梯度还是不同家系水平，Chl、Chla、Chlb、Chla/Chlb、Caro、Caro/Chl均有极显著差异；从正常水分至水分胁迫初期Chl、Chla、Chlb、Chla/Chlb稍微下降，后随着水分胁迫程度的加深下降较快，从正常水分至水分胁迫初期Caro、Caro/Chl先稍微下降，胁迫中期上升，胁迫后期下降；所有半同胞家系的Chl、Chla、Chlb、Chla/Chlb、Caro高于普通种，在水分胁迫条件下家系464、1027的Chl、Chla、Chlb、Chla/Chlb、Caro高于家系的平均水平，家系609、46的Caro/Chl值高于家系的平均水平。

Saying nothing, the man took a bottlenecked gourd, placed it on the ground, and put a coin with a square hole in the center over the top. He poured toil from a wooden ladle drop-by-drop into the gourd, without spilling a sinle drop on the coin. The man then turned to Chen Yau-tz and said,"That was also nothing. It's just a matter of practice makes perfect."Thus, the meaning of this idiom is that skill comes from repeated practice.

卖油的不说一句话，随手拿出一个葫芦放在地上，用一个方孔的铜钱盖在葫芦口上慢慢地用木杓子装满了油滴下去，只见油从钱孔中滴进去，可是铜钱上面一点儿油也没有沾上，这时卖油的才说：「我这也没甚么，只是熟能生巧罢了。

If you were not , and OS X just booted normally, have no fear.

如果你没有，和OS X刚刚启动通常，没有恐惧。

I only want to preserve our all .

我只想保存好我们的一切