查询词典 digest medium

A human promyelocytic leukemia cell mutant (HL-60-AR) with deficiency in HGPRT and resistance to 8-AG was established by treat〓nt of the HL-60 cells with MNNG and prescreened in 0.3% agar RPMI-1640 semisolid medium containing 8-AG. The mutant cells show the following characteristics:(1) resistant to cytotoxic action of 8-AG and growing vigorously in medium containing 20ug/ml 8-AG;(2) capable of forming 〓〓 colonies in soft agar medium in the presenee of 8-AG:(3) highly sensitive to HAT selective medium and fail to growing in HAT medium of form colonies in soft agar medium containing HAT;(4) completely deficinet in HGPRT activity; and (5) no radioactive silver grains over the mutant cells due to the absence of -hypoxanthine incorporation showed by radioautography.

一、用MNNG诱变和8-AG进行抗性筛选，成功地建立了一株抗高浓度8-AG和完全缺失HGPRT活性的人早幼粒白血病细胞突变株(HL-60-AR)，该细胞能抵抗8-AG的毒性作用，对HAT选择培养基十分敏感，检测不到HGHRT活性，不能参入〓H一次黄嘌呤。

The BMSCs were divided into six groups after repeatedly passaged: A,the BMSCs were cultured with conventional culture fluid(DMEM culture fluid+20%fetal bovine serum+2 mmol/L aminoglutaric acid amine) all the time;B,the BMSCs were cultured with conventional culture fluid+HGF(25ng/ml)+dexamethasone10~(-7M;C(HGF and Zuoguiwan induced group), the BMSCs were cultured with conventional culture fluid+ HGF(25ng/ml)+ dexamethasone10~(-7M+ 10%Zuoguiwan drug serum;D(conditioned medium and contrast serum induced group), the BMSCs were cultured with conventional culture fluid+50 % conditioned medium+10 % normal rat serum;E(conditioned medium and Bazhentang drug serum induced group), the BMSCs were cultured with conventional culture fluid+50 % conditioned medium+10 % Bazhentang drug serum;F(conditioned medium and Zuoguiwan drug serum induced group), the BMSCs were cultured with conventional culture fluid+50 % conditioned medium+10% Zuoguiwan drug serum.

常规培养组始终使用常规培养液(DMEM培养液+体积分数20％胎牛血清+2mmol／L谷氨酸胺)进行培养；HGF诱导组以常规培养液+促肝细胞生长因子(HGF，25ng／ml)和地塞米松10~(-7M进行培养；HGF加左归丸组以常规培养液+促肝细胞生长因子(HGF，25ng／ml)和地塞米松10~(-7／M+10％的左归丸含药血清进行培养；条件培养液加对照血清组以常规培养液+50％的条件培养液+10％正常大鼠血清进行培养；条件培养液加八珍汤组以常规培养液+50％的条件培养液+10％八珍汤含药血清进行培养；条件培养液加左归丸组以常规培养液+50％的条件培养液+10％左归丸含药血清进行培养。

The hFCECs were cultured by sticking tissues piece method that the cornea were divided into the limbus and central tissue piece and digestive method,respectively.For digestive method,the digestive juice of 5mg/ml DispaseⅡ+0.25%trypsin was chosed.D/F12 with 10%fetal bovine serum and 100 U/ml penicillin and streptomycin were used in this study,the culture condition was 37℃and 5%CO2,and culture medium changed every three days2、Passage of hFCECsAfter more than 80%confluenced,cells from corneal limbus hFCECs were digested for 5-15min with different concentrations of trypsin/EDTA at 37℃,and then passaged at a ratio of 1:2.The cells were subcultured on empty plates,mouse 3T3 fibroblast feeder layer,fetal corneal stromal cell feeder l ayer or HTK feeder layer respectively.And D/F12, mouse 3T3 fibroblast conditioned medium,fetal corneal stromal cells conditioned medium or HTK conditioned medium with 10%FBS and 100 U/ml penicillin and streptomycin were used respectively as the culture medium.And the culture medium were changed every three days as well.

方法一、人胎儿角膜上皮原代和传代培养1、原代培养严格无菌操作获取人胎儿角膜片，采用组织块贴壁法、酶消化法原代培养人胎儿角膜上皮细胞。2、传代培养角膜缘部的细胞生长融合达80%以上，不同稀释浓度的胰酶/EDTA消化液消化传代分别接种于空板、小鼠3T3成纤维细胞饲养层、胎儿角膜基质细胞饲养层及HTK饲养层，培养液分别采用D/F12、小鼠3T3成纤维细胞条件培养液、胎儿角膜基质细胞条件培养液、HTK条件培养液。

Where sterility test media are to be used in the Direct Inoculation of the Culture Medium method under Test for Sterility of the Product to be Examined , modify the preparation of Fluid Thioglycollate Medium and the Soybean–Casein Digest Medium as follows.

当无菌检查培养基用于供试产品无菌检查项下的培养基直接接种法时，按如下内容变更巯基醋酸盐液体培养基和大豆-酪蛋白消化物培养基的制备方法。

The results indicated:0.1 mg/L 2,4-D or 0.1 mg/L 2,4-D+0.2 mg/L KT was optimal to induct the callus of carrot hypocotyls.The majority of the callus induced on the medium containing 0.1 mg/L 2,4-D regenerated embryoid,whereas,the callus induced on the medium containing 0.1 mg/L (2,4-D)+0.2 mg/L KT produced adventive buds.During the growth of regenerated plant,MS medium is available for rooting of seedling,and seedling regenerated on B5 medium must be placed on the B5 medium containing 0.1% IBA to develop root.

试验结果表明：0 1mg/L2，4 D或0 1mg/L2，4 D+0 2mg/LKT的激素组合有利于胡萝卜下胚轴的愈伤组织诱导；在0 1mg/L2，4 D的培养基上形成的愈伤组织主要以胚状体的形式再生，而在含0 1mg/L2，4 D+0 2mg/LKT激素组合的培养基上形成的愈伤组织主要以发生不定芽的方式再生；再生过程中，B5基本培养基上的苗子不易生根，需要附加0 1mg/L的IBA，而在MS培养基上苗子可直接形成根。

A first recording medium (1) containing a content encrypted by a content encryption key, a portable second recording medium (2), a terminal (10) to which the first recording medium and the second recording medium can be detachably attached, a communication device (20) to which the second recording medium can be detachably attached, and a server (40) connected to a communication device.

配备存储用内容密钥加密的内容的第1存储介质(1)、可搬运的第2存储介质(2)、可装卸第1存储介质和第2存储介质的终端(10)、可装卸第2存储介质的通信装置(20)、以及连接通信装置的服务器(40)，终端将用公开钥对通信钥和持有人ID加密后的第1加密数据写入第2存储介质，安装于通信装置，并送到服务器。

Seed explants of Eleutherococcus senticosus produced somatic embryos directly ongrowth regulator-free medium. The results were showed that use low temperature stressmethod to relieve the Eleutherococcus senticosus seed conformation dormancy andphysiological dormancy, to induce seed germinate. Proliferation of Eleutherococcus senticosussomatic embryo without any plant growth regulathors was better in liquid medium containing1/3MS+sucrose 3%, and better in solid medium containing 1/3MS+sucrose 1%.Eleutherococcus senticosus secondary embryo was stick to matrix through espial of cytology.Eleutherococcus senticosus somatic embryo was cultivated some times to form immature frondin liquid medium, then transfer into solid medium to form full plant. The experiment wasindicated that much more Eleutherococcus senticosus immature frond can be cultured inbioreactor, so large-scale production in bioreactor culture can be done.

研究结果表明：低温胁迫处理刺五加种子可以打破种子的形态休眠和生理休眠，促使种子发芽：在不添加任何植物激素的培养基中诱导刺五加体胚形成，其中，1／3MS+3％蔗糖的培养基搭配有利于液体培养的刺五加体胚增值，1／3MS+1.0％蔗糖的培养基搭配有利于固体培养的刺五加体胚增值；在进行体细胞胚增殖时，体细胞胚继代三周后表面开始形成很多次生胚，对刺五加次生胚进行细胞学观察，发现刺五加次生胚与母体只是粘贴在一起；刺五加体胚需在液体培养基中培养一段时间形成幼植物体，实验表明可以通过生物反应器培养大量的刺五加幼植物体，然后转入固体培养基中形成完整植株，因此可以通过生物反应器培养进行规模化生产。

In the spiral pipe heat exchanger exports terminal, the ammonia steam temperature has been heated up 85 ℃--95 ℃, by now, the ammonia steam had the 45kg-55kg/cm2 ammonia steam pressure, sent in the ammonia steam turbine through the constant temperature pipeline, impelled ammonia steam turbine revolving, led the generator electricity generation;After the ammonia steam makes the merit release energy, the temperature drop, the returns ammonia storage tank, passes through again adjusts the press pump to press into the spiral pipe-type heat exchangers to carry on the next circulation;Including the ammonia steam turbine entire ammonia steam road is becomes the independent closed cycle system, is isolates completely with the outside air;The ammonia steam only plays the carryhome and the shift energy role, in the electricity generation process does not consume the actuating medium, the stored energy carrier water also is only gets up the carryhome and the shift energy function,The waterway also is from becomes the independent closed cycle system, in the electricity generation process also the needless water consumption, through the actuating medium ammonia steam and the stored energy carrier water unceasing shuttle service, transforms through the heat interchanger the solar energy as the actuating medium ammonia steam heat energy and the kinetic energy,With the aid of the ammonia steam turbine heat - machine transformation function and the generator machine - electricity transformation function, has realized the solar energy hot - electricity entire conversion process, transforms continuously the solar energy into the electrical energy, power supply for foreign;The low temperature generating system must solve three big technical keys:One, the anticorrosion (has actuating medium has strong corrosiveness), two is Explosion-proof (Some actuating medium Can have the detonation with the air mix when divulging, controls warm malfunction, elevates temperature suddenly also can have detonation), three is guards against the revolution axis divulging

以太阳能低温发电系统为例，低温发电方法是这样进行的：以水作为储能载体的太阳能采集器将太阳能采集起来，将水温提升至85℃--98℃；用氨作为工作介质储于氨储罐及氨汽路中；采用螺旋管型热交换器；用调压泵将氨储罐中的氨汽压入螺旋管型热交换器的螺旋管内，用调压泵将携带太阳能的85℃--98℃的储能载体热水压入螺旋管型热交换器的螺旋管外壁空间进行循环式热交换，在热交换器的螺旋管出口端，氨汽温度已被加热到85℃--95℃，这时，氨汽具有45kg—55kg/cm2的氨汽压力，通过恒温管道送入氨汽轮机，推动氨汽轮机旋转，带动发电机发电；氨汽作功释放能量后，温度下降，返回氨储罐，再经调压泵压入螺旋管型热交换器进行下一次循环；包括氨汽轮机在内的整个氨汽汽路是自成独立的封闭循环系统，与外界空气是完全隔绝的；氨汽只起携带和转移能量的作用，发电过程中并不消耗工作介质，储能载体水也是只起携带和转移能量的作用，水路也是自成独立的封闭循环系统，发电过程中也不消耗水，通过工作介质氨汽和储能载体水的不断循环运行，通过热交换器将太阳能转化为工作介质氨汽的热能和动能，借助氨汽轮机的热—机转化功能和发电机的机—电转化功能，实现了太阳能的整个热—电转化过程，将太阳能源源不断地转变为电能，对外供电；低温发电系统要解决的三大技术关键：一是防腐（有的工作介质具有较强的腐蚀性）、二是防爆（有的介质泄漏与空气混合会产生爆炸，控温失灵，急剧升温也会产生爆炸）、三是防轴漏（汽轮机是动态旋转体，必须解决工作介质的防轴漏问题）。

The inducement medium was as follows respectively: for the leafstalk, B5 medium with 6-BA 0.5 mg/L+IBA 0.1 mg/L, and for stems, B5 medium with 6-BA 1.0 mg/L+IBA 0.2 mg/L, and subculture medium both were B5 medium with 6-BA 1.0 mg/L+2, 4-D 0.1 mg/L.

利用叶柄和茎获得松散愈伤组织时，其诱导培养基组成分别是：B5培养基+6-BA0.5mg/L+IBA0.1mg/L和B5培养基+6-BA1.0mg/L+IBA0.2mg/L，其继代培养基均为B5培养基+6-BA1.0mg/L+2，4-D0.1mg/L。

There is no way to cross into this dream without matching the fullness of sound within one's energy flow, which has extreme high notes, medium high notes, low high notes, high medium notes, medium-medium notes, medium low notes, high low notes, low-low notes, and extreme low notes.

除了在你能量流中完全匹配上这一声音以外，并没有其他方法进入到这一个梦想之中，它有极高音、中高音、低高音，高中音、中中音、低中音和高低音、中低音、极低音。

I'm not an actor. I'm a professor of paleontology.

我不是演员，我是古生物学教授

Spider Network Web site that is a very image of the name.

网络蜘蛛即Web Spider，是一个非常形象的名字。