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Nutrition solution culture, ICP-AES, the differential centrifugation technique and sequential chemical extraction method, suppression subtractive hybridization , RAPD were used in this thesis to study the mechanisms of copper tolerance and accumulation by Commelina communis grown in copper mine deposit which was reported to be a Cu hyperaccumulator, compared with C. communis grown in normal soil. Several aspects were examined, including: difference of copper tolerance and accumulation between C. communis grown in copper mine deposit and that grown in normal soil; difference of growth and nutrient accumunation between C. communis grown in copper mine deposit and that grown in normal soil under copper treatment; physiological differences between between C. communis grown in copper mine deposit and that grown in normal soil under copper treatment; subcellular distribution and chemical forms of Cu in C. communis; identification of cDNA fragments differentially expressed in C. communis grown in copper mine deposit; genetic diversity and copper tolerance of C. communis, tolerance mechanisms to copper in C. communis are discussed.

中文题名鸭跖草对铜的耐性机理和富集特征研究副题名外文题名 Mechanisms of Cu tolerance and accumulation by Commelina communis 论文作者廖斌导师蓝崇钰束文圣学科专业环境科学研究领域\研究方向学位级别博士学位授予单位中山大学学位授予日期2003 论文页码总数128页关键词重金属污染植物修复超富集植物土壤污染铜污染鸭跖草馆藏号BSLW /2003 /X53 /12 论文以本实验室报道的生长于矿山上的铜超富集植物鸭跖草和生长于正常土壤中的鸭跖草为试验材料，分别运用溶液培养法、ICP-AES元素测定技术、差速离心法和化学试剂逐步提取法、抑制消减杂交技术、RAPD技术研究了矿山和非矿山两种生境鸭跖草对铜的耐性和吸收积累的差异，两种生境鸭跖草在铜胁迫下养分吸收和积累的差异，两种生境鸭跖草在铜胁迫下生理反应的差异，以及铜在鸭跖草细胞内的分布及结合形态研究，矿山鸭跖草差异表达的cDNA克隆，铜对鸭跖草种群遗传分化的影响，从这几个方面探讨了矿山鸭跖草对铜的耐性机理。

In service organization,disparate facets of service can be differentially conformant for customers,and, consequently service facilities must be systematized so that they lay stress on the relevant service facets.

In服务组织的目标的最好，服务的不同方面可以可为客户提供差异conformant，而且，因此服务设施，必须制度化，使他们注重相关服务方面的压力。

Two-dimensional electrophoresis (2-DE) was used to study the variation expression of proteins of cucumber cotyledonary nodes induced with PP333. The characteristics and functions of differentially expressed proteins were analyzed with MALDI-TOF-TOF/MS and Protein Data Bank.

应用双向凝胶电泳技术研究PP333处理后黄瓜离体子叶节培养物差异蛋白的表达，结合质谱分析和蛋白质数据库检索，确定所检出差异蛋白质的性质和功能。

As the changes reflect some genes expressed herein, identification and characterization of the differentially expressed genes will be helpful to understanding the molecular mechanism of erythroid terminal differentiation.

这些表型的变化反映了一系列内在基因的开启和关闭。显然这些基因的鉴定与克隆对阐明红系细胞终末分化的机制有重要意义。

Methods Differential display reverse transcription polymerase chain reaction was carried out to screen the differentially expressed gene fragments from the lateral geniculate body and the visual cortex.

利用差异显示反转录PCR技术分析大鼠外侧膝状体与视皮层基因差异表达情况，并筛选差异表达基因片段。

With the development of new high-flux experiment technology—proteomics, we could wholy、 dynamically and interrelatedly investigate the differentially expressed proteins among chronic sinusitis, nasal polyps and normal nasal mucosa tissue.

随着蛋白质组学这一新兴科研方法的出现，使我们能在同一实验体系中高通量筛选慢性鼻窦炎、鼻息肉、鼻息肉病和正常鼻粘膜组织间差异表达的蛋白质，从而探索不同病变组织之间生命活动的差别。

Among the six ESTs obtained through SSH analysis, DS69 and DS205 did express differentially between the Rht-Blc isogenic lines.

在6个通过SSH分析获得的ES7s中，DS69和DS205的表达在近等基因系间确实表现出差异。

Gene fragments expressed differentially in Kandelia candel at two salt levels were identified by improved representational difference analysis of cDNA .

利用改进的代表性差异分析技术，从红树植物秋茄中分离了6个受盐胁迫诱导表达的基因片段和5个受盐抑制表达的基因片段。

Representational difference analysis of cDNA is a powerful technique for cloning the differentially expressed genes between samples. Kandelia candel and Dunaliella salina are two important halophytes.

cDNA RDA技术是目前应用较为广泛的一种分离差异表达基因的方法，盐藻和秋茄是两种重要的盐生植物。

Several lactobacilli possess multiple surface layer protein genes can be differentially or simultaneously expressed.

一些乳酸菌含有多种S-层蛋白编码基因，他们能够分别或同时表达。

They have quite a lot of work to do.

他们有许多工作要做。

Let us give you a more elaborate example of this process.

让我们给你这个过程的更多的详细例子。