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A total of 653, 532, and 459 unqiue tags were differentially expressed at 65, 33, and 90 days of gestation between Landrace and Tongcheng pigs, respectively.

通城猪和长白猪之间差异表达标签数最多的是在妊娠65天，有653个单标签。

N-methyl-N＇-nitro-N-nitrosoguanidine was used as a known teratogen to induce cleft palate in ICR mice and a suppression subtractive hybridization technique was applied to identify differentially expressed genes which related to cleft palate in ICR mice.

方法以甲基亚硝基胍为受试物诱导腭裂动物模型，采用抑制消减杂交技术从全基因组水平上对MNNG致腭裂相关差异表达基因进行筛选。

Ultrasonic imaging method combined with water bag in rectum is superior to that of only TAUS or TVUS in diagnosing and differentially diagnosing masses behind uterus,which has significant clinical value.

超声显像加用直肠水囊法对女性子宫后方包块的诊断和鉴别诊断方面明显优于单纯应用超声显像者，具有较高的临床价值。

In order to study themolecular and regulation mechanism deeply, in this study, based on constructing suitableNILs of total RNA pools of opposite maize and mutual maize, singleendonuclease digestion cDNA-AFLP technology was applied to screen , isolation andbioinformatics analyze the gene differentially expressed fragments related to the oppos...

为了深入地研究这一珍贵突变材料形成的分子机理和调控机制，本研究中，通过构建合适的玉米对生、互生近等基因系总 RNA池，采用改进的单酶切cDNA-AFLP 技术，对扩增的玉米对生性状相关基因的差异表达片段进行筛选、分离和生物信息学分析。

A well-resolved and reproducible 2-DE pattern of PBMC from patients with HBV related HCC or LC is established, and sixteen differentially expressed proteins were characterized.

建立了分辨率高且重复性较好的HBV相关性HCC及LC患者PBMC双向凝胶电泳图谱，并识别鉴定出了一些与HBV相关性HCC癌变相关的差异表达的蛋白质。

The well-resolved and reproducible 2-DE pattern of Splenolymphocyte were established in rat with cerebral hemorrhage and cerebral ischemia and ten differentially expressed proteins were characterized.This approach may lay a foundation for the further investigation of the relationship between cellular immune function and cerebrovascular disease injury.

建立了分辨率高重复性较好的脑出血及局灶性脑缺血脾淋巴细胞总蛋白的双向凝胶电泳图谱，并鉴定一些与脑血管病脑损伤相关的差异表达蛋白质，为深入研究脑血管病细胞免疫功能改变与脑血管病之间的关系奠定了基础。

Results Well-resolved, reproducible 2-DE profiles of human colon carcinoma tissues and paired normal tumor-adjacent colon tissues were obtained. For colon carcinoma tissues, a total of (980±28) spots were detected; for paired normal tumor-adjacent colon tissues,(860±45) spots were detected. For normal tumor- adjacent colon tissues, the average deviation of spot position was (10.487±0.11) mm in IEF direction and (0.944±0.12) mm in SDS-PGE direction; for colon carcinoma tissues the average deviation of spot position was (0.654±0.11) mm in IEF direction and (1.20±0.22) mm in SDS-PGE direction. Number of differentially expressed proteins was 72.00±12.34 between colon carcinoma and paired normal tumor-adjacent colon tissues.

结果 建立了结肠癌及正常肠组织的双向凝胶电泳图谱，其中癌旁正常结肠组织和结肠癌组织电泳图谱中平均蛋白质点数分别为860±45和980±28；癌旁正常结肠组织和结肠癌组在IEF方向上的平均偏差分别为(0.487±0.11)mm和(0.654±11)mm，在SDS-PAGE方向上的平均偏差分别为(0.944±0.12)mm和(1.20±0.22)mm，结肠癌与癌旁正常结肠组织的差异表达蛋白质点数为72.00±12.34。

In maize, the results indicated that the patterns of gene expression altered obviously in cross-fertilized kernels as compared to self-fertilized kernels, major difference of gene expression occurred in the qualitative level. Further analysis indicated that higher percentage of total differentially expressed cDNA was observed in highly heterotic hybrid than in non-heterotic hybrid.

对四个不同优势的玉米杂交和自交在种子发育前期基因表达研究表明，玉米杂交种子与自交种子在发育前期存在明显的基因表达差异，强优势的杂交组合与弱优势杂交组合之间表现差异的总cDNA比例存在极显著差异，进一步分析发现，杂交特异表达与杂种优势存在一定的正向关系。

Homogenates were centrifuged differentially, first at 8000g for 7 min, then at 17,000g for 11 min, and finally at 27,000g for 5 min at 4 °C. Protein content was determined in the supernatant as per Lowry et al.

匀浆离心的差异，首先在八千克为7分钟，然后在一点七○○万克为11分钟，最后在二点七〇万克为5分钟在4 ℃的温度蛋白质含量是确定的上清液作为每劳瑞等人。

Mitochondrion proteins of normal and ischemic heart tissues were separated using 2DE and 3 differentially expressed proteins were found.

比较蛋白质组学技术在人类疾病研究中具有重要的作用。为了研究心肌缺血造成心肌损伤的机理，我们首次利用蛋白质组学方法对缺血心肌的线粒体进行了研究。

Who? I never heard of him, Paul said, before asking teammate James Posey if he had heard of him.

赛后，科比说，他一直都是一名非常出色的射手，今天他打得很棒。

When I joined the company, I rotated around the different sections.

我加入这个公司时，轮换过几个不同的部门。