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The 4th left rib was cut and a model of left coronary artery occlusion/release was carried outo Rats were divided randomly into 4 groups: control group without LCA occlusion (group A, n=6);I/R group(group B, n=6); ketamine(5mg.kg-1)+I/R group (group C, n=6); ketamine(10mg.kg-1)+I/R group(group D,n=6)o All rats in groups B, C , D were subjected to 30 minutes of LCA occlusion followed by 120minutes of reperfusion. Rats in group C and D were injected with 5mg.kg-1 and 10mg.kg-1 of ketamine before reperfusion, respectively. Significant electrocardiogram and color changes at the area at risk were considered indicative of successful coronary occlusion and reperfusionc Serum was exampled from left jugular vein at 30 minutes and 120 minutes during reperfusion to measure IL-6 and TNF- level by ELISA measurements. After reperfusion of 120 minutes, the heart was removed and the cardiac apex was exampled with snap-frozen in liquid nitrogen and stored at -70 C .

采用左冠状动脉前降支结扎开放建立心肌缺血／再灌注模型，健康SD大鼠24只，随机分为心包打开假手术组(A组，n=6)，缺血／再灌注对照组(B组，n=6)，5 mg·kg~(-1)氯胺酮+缺血／再灌注组C组，n=6，缺血30min后右腹股静脉注入5 mg·kg~(-1氯胺酮，10mg·kg~(-1)KTM+I／R组D组，n=6，缺血30min后右腹股静脉注入10 mg·kg~(-1氯胺酮。10％水合氯醛40 mg·kg~(-1)腹腔注射麻醉，气管切开，连接多功能监护仪记录心电图；小动物呼吸机人工呼吸，呼吸频率60次／分，潮气量2ml／100g，于左胸第四肋间打开胸腔暴露心脏，在左心耳下1mm左冠状动脉处，用丝线，眼科外用不锈钢小圆针穿过心肌浅层，稳定10min后将U型含有铜丝的胶管置于冠状动脉表面一起结扎(A组不结扎，B、C、D组结扎)；结扎开始左心室心尖部即由红色变暗，30 min后呈暗红色，心电图中出现S-T段抬高，说明缺血形成。

Results The recovery time of bowel sound was 8-112 h (M=28 h) in the treated group and 16-24 h (M=46 h) in the control group, with a significant difference P=(0.0001. The time of anal exsufflation in the treated group was 2-116 h (M=56 h), and 24-147 h (M=82 h) in the control group, with a significant difference (P=0.0001). The electroenterogram revealed that control activity, continuous response activity and contractive electrical complex were significantly stronger in the treated group than in the control group.

结果 187例经观察(治疗组93例，对照组94例)，肠鸣恢复时间治疗组为8～112h，中位时间为28 h，对照组为16～124 h，中位时间46 h，有显著统计学意义(P=0.0001)；肛门排气时间治疗组为22～116 h，中位时间56 h，对照组为24～147 h，中位时间82 h，有显著统计学意义(P=0.0001)；肠电图显示治疗组各检测肠段的电控制活动、连续反应电活动波、收缩电综合波均显著增强。

Equidistance point and difference theory in theory of function approximation are studied. Meanwhile, the relation among difference, difference quotient and derivate is revealed. By drawing Lagrange's and Cauchy's theorem of mean on difference and Taylor's formula into difference function, four theorems, such as Lagrange's theorem of mean on difference, are concluded in simple way. On the basis of these conclusions, the asymptotic property of middle point is studied, a series of new conclusions are drawn and the discussions on the asymptotic property of middle point in differential mid-value are summarized.

对函数逼近论中等距节点和差分理论进行了研究，揭示了差分、差商与导数之间的联系；将Lagrange中值定理、Cauchy中值定理、Taylor公式引入到差分函数中，简明地推导出Lagrange差分中值定理等4个定理，并在此基础上对"中间点"的渐近性进行了研究，得出了一系列"中间点"的渐近性的结果，概括了有关文献对微分中值公式的"中间点"的渐近性的讨论；给出的引理改进了函数逼近论的证明方法，精简了函数逼近论中的一些内容。

The results showed:among the 7 isolates, five isolates of HY3、GY1－3、ZJ1－1、HP1、FC3 had same colony shape, irregular shape, liquidlike, slimy, opacity with smooth surface;the other two isolates had same shape, irregular shape, dry, opacity with coarse surface. By inoculating eucalyptus with the 7 isolates, the plants were infected apparently, and the young plants of eucalyptus in control experiment with tap water were not infected. By cultivating eucalyptus cuttings with the bacterial suspensions without EPS, the incidence of disease was very distinct,but compared with the former bacteria suspension,the incidence of disease has decreased at different degrees. By screening out two isolates of strong pathogenicity and two isolates of weak pathogenicity from the 7 isolates,making the bacterial suspensions with them to inoculate the young plants of eucalyptus, two treatments of cutlings and ramets with rats were set with 5 repetitions in every treatment, the results of data analysis showed: for the cutlings, the bacterial contents in upper and middle parts、upper and lower had significant difference;for ramets with roots, the bacterial contents in upper, middle parts, lower had significant difference between each other; For both the cutlings and ramets with roof, the bacterial contents in xylem and phloem had significant difference. The interaction between vertical and horizontal parts for the bacterial content had significant difference. For the two isolates of HY3 and 93B which were screened out at last,their activities of the cellulase were: 1.955ug/ and 1.288ug/ respectively, and had significant difference; the activities of pectase were: 1.325 ug/and 1.24ug/ respectively, and had no significant difference. The content of EPS extracted from the two isolates of HY3 and 93B was very different: 7.08x10-8ug/cell and 5.17x10-8ug/cell.

结果显示：7个菌株中，其中5个菌株HY3、GY1－3、ZJ1－1、HP1、FC3的菌落形态相同：不规则形状、流体、粘性、不透明、表面光滑；另外2个菌株93B、GN1菌落形态相同：不规则形状、干燥、不透明、表面粗糙；用7个菌株接种剪根桉树苗，发病情况非常明显，而自来水对照实验中桉树苗却不发病；无EPS菌悬液培养桉树剪根苗，发病率也很明显，但是相比原菌液，则发病率有不同程度的下降；从7个菌株中间筛选出来2个强致病性菌株和2个弱致病性菌株，用它们配制菌悬液培养桉树苗，设置剪根和不剪根两个处理，每个处理设置五个重复，数据分析结果显示：对于剪根苗，上部和中部、上部和下部的含菌量有显著的差异，中部和下部含菌量差异不显著；带根苗，上部、中部、下部含菌量彼此之间差异显著；不管是剪根苗还是带根苗，木质部和韧皮部含菌量之间的差异都非常显著；上中下与木韧交互作用中，含菌量差异非常显著；最后筛选出来的强弱2个菌株HY3和93B，它们的纤维素酶活性分别为：1.955ug/和1.288ug/，具有显著的差别；果胶酶的活性分别为：1.325 ug/和1.24ug/，没有显著的差别，而且HY3和93B两个菌株细胞分泌的胞外多糖的含量差异很显著，分别为：7.08×10-8ug/cell和5.17×10-8ug/cell。

Equidistance point and difference theory in theory of function approximation are studied. Meanwhile, the relation among difference, difference quotient and derivate is revealed. By drawing Lagrange's and Cauchy's theorem of mean on difference and Taylor's formula into difference function, four theorems, such as Lagrange's theorem of mean on difference, are concluded in simple way. On the basis of these conclusions, the asymptotic property of middle point is studied, a series of new conclusions are drawn and the discussions on the asymptotic property of middle point in differential mid-value are summarized.

对函数逼近论中等距节点和差分理论进行了研究，揭示了差分、差商与导数之间的联系；将Lagrange中值定理、Cauchy中值定理、Taylor公式引入到差分函数中，简明地推导出Lagrange差分中值定理等4个定理，并在此基础上对&中间点&的渐近性进行了研究，得出了一系列&中间点&的渐近性的结果，概括了有关文献对微分中值公式的&中间点&的渐近性的讨论；给出的引理改进了函数逼近论的证明方法，精简了函数逼近论中的一些内容。

Results (1) Compared with that of 4-Retro-decoction group and heart failure control group, the activity of Na〓-K〓-ATPase in low- and middle- dosage ginger group showed to be decreased with statistically significant difference. P＜0.05.(2) The activity of Ca〓-ATPase and Mg〓-ATPase in the lower- and middle-dosage ginger group were not statistically different from that of 4-Retro-decoction group and control group, but the activity of Ca〓-ATPase showed an ascending tendency in low-dosage ginger group.

结果 (1)低、中剂量干姜组的Na〓-K〓ATP酶活性明显偏低，与四逆汤组和心衰组比较，差别均具有显著性，P＜0.05；(2)低、中剂量干姜组的Ca〓-ATP酶和Mg〓-ATP酶活性与四逆汤组和心衰对照组比较，无统计学意义，但低剂量干姜组的Ca〓-ATP酶与其它三组相比有升高趋势。

In the group of treatment with ZKK,the AST,ALT and the lipid in the liver decrease significantly and differ from the natural recovery group'sbut the normal group's.In the group of treament with Simvastatin,the affection to the ALT,AST and the lipid in liver is no apparent,there is no difference campared to that of recovery group,but very difˉferent from that of treatment group with ZKK,In the combined treatment group,the effect to decrease the bloodlipid is significant,the influence on liver is similar to the ZKK's.Conclusion Rich-fat food can induce dyslipidemia,atheromatous patch and hepar adiposum.

辛伐他汀组对成模时增高的ALT、AST和肝组织中的脂质及脂肪肝影响不大，与模型组相比无差别；之可康组和联合治疗组均可明显降低成模时升高的AST和ALT及肝组织中的脂质，逆转脂肪肝，与正常组相比无差别，与模型组、辛伐他汀组分别相比差异有显著性；联合用药组血脂水平调节幅度最大，对肝脏的影响与之可康组相比无差别。3个治疗组均可使动脉硬化斑块回缩。

Results showed:(1) The airway responsiveness of smoke control group was higher than that of normal control group, the airway responsiveness of smoke + exercise group was lower than that of smoke control group;(2) Plasma level of cortisol determined immediately after exercise was higher than that determined before exercise, no significant difference occurs between that determined next day morning after all exercise finished in contrast to that determined before exercise;(3) HE staining showed, there was severe chronic pulmonary inflammatory response in smoke control group, which was reduced in the smoke + exercise group;(4) chronic cigarette smoke downregulated the protein and mRNA expression of BKca in smooth muscles of bronchi and bronchioli, exercise increased the mRNA expression of BKca;(5) chronic cigarette smoke downregulated the protein and mRNA expression of Kv1. 5 in smooth muscles of bronchi and bronchioli, exercise increased both the protein and mRNA expression of Kv1. 5 in contrast to smoke control group in bronchioli and did not effect the expression of Kv1. 5 in bronchi;(6) chronic cigarette smoke induced upregulation of FIZZ1/RELMα in bronchial smooth muscle cells, exercise decreased these effect.

结果：(1)吸烟对照组的气道反应性明显增高，吸烟加运动应激可明显降低大鼠的气道反应性；(2)吸烟运动组运动后血浆皮质醇浓度明显高于运动前，但全部运动结束后次日晨测定的血浆皮质醇浓度与运动前无明显差异；(3)HE染色显示，吸烟对照组肺组织出现明显的慢性炎症反应，吸烟加运动应激组比吸烟对照组炎症反应轻；(4)慢性吸烟可降低大鼠大气道和小气道BKca mRNA和蛋白表达，而吸烟加运动应激可使BKca mRNA表达上调，但对小气道的蛋白表达无影响；(5)慢性吸烟可降低大鼠大气道和小气道Kv1.5蛋白和mRNA表达，在小气道吸烟加运动应激可减轻这种作用，大气道则无作用；(6)吸烟使支气管平滑肌的FIZZ1/RELMα蛋白表达明显增强，而吸烟加运动组的RELMα蛋白及mRNA均明显减弱。

RESULTS: FGFR1-positive chondrocytes were abundant in the maturative layer and hypertrophic layer, but only few FGFR1-positive chondrocytes were found in the proliferative layer. In the control group, the expression of FGFR1 increased from 6-week-old to 10-week-old rats, and then decreased and went stable. In both young and adult group, the expression of FGFR1 in the experimental group was significantly lower at 4, 6 weeks and higher at 8 weeks than that in the control group, especially in the young group (P.05). But no difference was found between the experimental group and the control group at 2 weeks in both groups. CONCLUSION: FGFR1 may play an important role in the remodling of condylar cartilage induced by occlusal disorders.

结果：FGFR1主要表达在大鼠髁突软骨过渡层和肥大层，增殖层很少；正常组大鼠髁突软骨从6周龄到10周龄FGFR1表达逐渐增强，10周龄后降低，并保持在一个稳定水平；成年组和幼年组实验4、6周时FGFR1的表达均显著低于对照组，实验8周时的表达高于对照组(P.05)，幼年组尤为明显，实验2周组与对照组无显著差异。

Moreover, the actual allele frequency of most varieties deviates far from Hardy-Weinberg equilibrium. All PPB、na 、I、h、Gi and Fst have proved to be the references to elucidate that ISSR is a most powerful tool to analyze genetic diversity, compared with the RAPD marker and the allozyme marker is less strong ordinally. We could divided the 70 samples into A, B, C, D and E five groups using three methods according to genetic distance clustering. There is a bit displacement for few varieties in different clustering maps, but the most are similar to morphological analysis despite that there is still a great difference among cultivars in the same one group. The above results imply that the three methods have the different sensitivity and resolution in genetic distance analysis of close varieties. The Mantel test indicates that the results from the three kinds of markers have the significant correlation, which demonstrates that the number of the used three kinds of markers is enough to exactly detect the diversity of all 70 samples to ideal extent. And these methods can be used to evaluate the diversity of the whole group using the miscellaneous samples instead of the individual sample, of the Gerbera jamesonii are mainly from tissue culture plants. In conclusion, the above study results provide a reference for the application of three kinds of molecular markers to molecular marker-assisted breeding of flower. 2. The genetic diversity among the eight introduced cut-flower varieties of Ranunculus asiatica was analyzed by the ISSR markers. Based on the genetic clustering tree, all the colorful flower varieties are clustered into one group, and the white flower varieties into another group. Moreover, among the former group the yellow flower varieties are clustered into one sub-group, and the reddish flower varieties, such as rose color, pink, nacarat, are clusetered into another sub-group.

由三种分子标记的分析结果可以看出，等位基因平均值、观察杂合度、Fis值、Fit值皆较高，表明非洲菊等位基因较丰富，杂合基因偏多，且绝大部份品种的实际等位基因频率在品种内偏离了Hardy-Weinberg equilibrium；PP8、na、Ⅰ、h、Gi及Fst皆表明，ISSR检测遗传多样性的能力最强，其次是RAPD，等位酶最低；根据遗传距离进行聚类，三种方法都把70个品种分成A、B、C、D、E五个大组，每一组中除少数品种发生位移外，大部份品种分类结果相似，且与形态分析结果有相似性，但在每一组中，品种间的聚类差别较大，表明这三种方法在近距离品种间检测遗传变异时灵敏度及分辨力不同；Mantel检测表明，三种标记的分析结果有显著相关性，表明所用的三种分子方法的标记数量已经可以相对无偏地检测到70个品种间遗传变异；非洲菊为组培苗，三种标记的检测结果皆表明，混合样品可以作为个体样品的代表，对整个居群的遗传多样性进行评价；这些研究结果可为三种分子标记方法在花卉分子辅助育种中的进一步应用提供借鉴。

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"第二次生命是显着容易的工作，并且很受欢迎，"

For example, we usually assume that materials are homogeneous and isotropic and free of internal defects or flaws.

为了得到适合有限元分析的模型，我们必须经过如图2所示的简化步骤。