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Total RNA was isolated from cultured human pigmental cells and mRNA was reversly transcribed into cDNA. Then PCR was used to amplify the TRP -2 coding region. The PCR product was cloned into pUC19 plasmid and sequenced, then subcloned into vector pGEX-4T-1. The TRP-2 protein was expressed hi E coli of DH5 a as fusion protein with glutathioneS-transferase induced by IPTG. The fusion protein was purified by glutathione resin column.

我们通过RT—PCR的方法扩增TRP—2编码基因，克隆至pUC19载体并测序，再亚克隆至GST融合表达载体pGEX—4T—1，转化大肠杆菌，IPTG诱导表达了GST/TRP—2融合蛋白，并分析鉴定表达蛋白的可溶性，最后第四军医大学硕士学位论文一用谷氨酚胺树脂柱纯化表达的蛋白。

In this algorithm, the product of two huge primes is the secret information, which is further converted into n natural numbers and then encoded into planted plane cubic tree structures which are embedded into the software codes as software watermark.

水印嵌入的入程是：首先将两个大质数的乘积采取中国剩余定理划分成n个天然数，然后再将这n个天然数编码入n个平面环路树PPCT构造中，将这些构造的构建代码嵌入软件代码中。

In this paper, we give explicit constructions and formulations for harmonic maps from R1,1 into classical real semisimple Lie groups by using Darboux transformation. We also discuss pluriharmonic maps from complex manifoldsinto symmetric spaces and Willmore surfaces in Sn. By converting geometric conditions satisfied by these maps into integrable systems, and using the the-ory of integrable systems, we give explicit constructions for pluriharmonic maps from complex manifolds into symmetric spaces and the Willmore surfaces in Sn respectively. Finally, we classify hypersurfaces in Sn+1 with three distinct prin-ciple curvatures and zero Mobius form using the theory of Mobius geometry. The paper consists of four chapters.

本文首先利用Darboux变换的方法给出了从Lorentz平面R~(1，1)到经典实半单Lie群的调和映照的具体构造，并给出其显式表示；其次研究了复流形到对称空间的多重调和映照及球空间S~n中Willmore曲面，将这些映照所满足的几何条件转化为可积系统，然后利用可积系统理论分别给出复流形到对称空间的多重调和映照与S~n中Willmore曲面的构造；最后利用Mōbius几何的理论给出S~(n+1)中具有三个不同主曲率且Mōbius形式为零的超曲面的分类。

Similar to Debye screening in QED, at sufficiently high density we expect colour screening to set in and the potential does not go into infinity as r increases. Color screening thus transform a color insulator into a color conductor, turning hadronic matter into a quark matter, where the interactions between quarks and gluons become relatively weak and long range.

在极高的能量密度下，类似于量子电动力学中的"德拜屏蔽"，夸克、胶子间的相互作用可能不再随距离的增大而趋于无穷大，夸克、胶子可以超出强子，在较大的范围内运动，形成夸克-胶子等离子体。

Lamin A gene cDNA lacking of 150 basyls at the end of the eleventh extron was cloned into pcDNA3.1 carrier, and the recombinant plasmid pcDNA3.1-mutant LMNA cDNA was injected into the male pronucleus of mouse zygotes, then the zytotes was replanted into the oviducts of pseudopregnant mice.

实验方法：将缺失了第11外显子末端150个碱基的LMNA基因cDNA克隆到pcDNA3.1载体，应用原核显微注射方法将线性化的pcDNA3.1-mutant LMNA注射到小鼠受精卵的雄原核，再将注射后的受精卵移植至假孕鼠的输卵管。

Living thus, he came by chance one day to a clear fountain, and lay down by it; when behold ing in the water his own image, he fell into such a study and then into such a rapturous admiration of himself, that he could not be drawn away from gazing at the shadowy picture, but remained rooted to the spot till sense left him; and at last he was changed into the flower that bears his name; a flower which appears in he early spring; and is sacred to the infernal deities ----Pluto, Proserpine, and the Furies.

就在这种生活中，有一天，他偶然来到一池清泉旁。正值炎热的中午，他便在泉边躺下。一看到池水中自己的倒影时，他便细细察看观赏，继而狂喜地恋慕自己。凝视着自己若隐若现的水中美影，他不能自拔，就像扎根于此，直到感觉消去。最后他变成以他名字命名的水仙花。水仙花于早春开放，是冥府诸神的圣花：普路托，普洛塞尔皮那，复仇三女神皆以此花作祭。

Article 98 Enterprises which must complete procedures for tax refunds in the case of overpayments of tax may, where income in foreign currency has already been converted into Renminbi according to the foreign exchange rate, convert the amount of the tax in Renminbi to be refunded into foreign currency according to the exchange rate in effect when the tax was originally paid, and then reconvert this amount of foreign currency into Renminbi according to the foreign exchange rate at the date of issuance of the tax refund certificate.

第九十八条企业所得为外国货币并已按照外汇牌价折合成人民币缴纳税款的，发生多缴税款需要办理退税时，可以将应退的人民币税款，按照原缴纳税款时的外汇牌价折合成外国货币，再将该外国货币数额按照填开退税凭证当日的外汇牌价折合成人民币退还税款

That is to say, the ritual of political legitimacy must be changed from traditional type of consanguinity, hereditariness and god-given regality into legal principle type in which the authority comes from and is approbated by people\'s vote; from subject type of passive moralized inculcation into citizen type of initiative study and free selection; from closed exclusive type into openparticipation type——the ritual of people participating in politicalactions is no longer controlled or confined in an extremely incapacious and closed spectrum.

政治合法化仪式从基于血缘、世袭和君权神授的传统型向以通过民众投票和选举来表达授权与认可的法理型转变；政治社会化仪式从被动教化和灌输的臣民型向主动学习和自由选择的公民型转变；政治参与仪式由封闭的排他型转变为开放的参与型——即参与政治的仪式的不再被控制和局限在极其狭小和封闭的范围内。

By means of Hua, filial duty is assimilated into the ceremony of ancestor worship, enabling the ceremony to bear the function of filial education; by means of cultivation, the individual gains the filial concept and behavior; the blood ties will be sustained and the unity and identification of the family members will be strengthened in the way of converting filial duty into the love, the respectfulness, the support and the worship to parents and the activities of repairing the clan temple and continuing the family tree. Finally, filial duty coming into the different forms of reading, playing and writing scripture finishes the entire society\'s integration and sustains the social stability.4. It is because the Bai People group passes on the filial duty in the principle of Cheng that the filial duty still has an effect on the individual, the family and the society.

通过"化"，孝道融入了祭祀祖先的仪式中，使该仪式具备了孝道教育的功能：通过"化"，个体获得了孝道的观念和行为；将"孝道"化为对父母的爱、敬、养、祭，以及修祠、续谱的活动中，就可以稳固血缘家庭关系，增强宗族成员彼此之间的认同、团结和凝集力；最后，孝道"化"到莲池会的诵经、洞经会的谈经和圣谕堂的写经活动中，完成了对整个社会的整合，维持了社会的稳定。4、孝道之所以仍旧在白族的个体、家庭、社会中发挥作用，是因为他们在传承孝道时，本着"诚"的原则。

By incarnation He brought God into man;by resurrection He brought man into God,that is, He brought His humanity into the divine sonship.

他怎样借着成为肉体，将神带到人里面，也照样借着从死人中复活，将人带到神里面，就是将他的人性带进神圣的儿子名分里。

Don not attempt to do something which you can not to do.

不要企图做那些办不到的事情。

The expression of CTGF and TNF-αweredetected by immunochemistry and the number of Clara Cells was calculated.

光镜下观察肺组织的病理变化，采用免疫组化染色观察肺组织中结缔组织生长因子和肿瘤坏死因子-α的表达和Clara细胞的数量。