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Drug sensitive test and three-dimensional test220 strains of Pa were isolated from hospitalized patients between 2003 and 2007. K-B method was used to tested the susceptibility of 10 different antibiotics. IRPa was screened by testing the minimal inhibitory concentration of imipemem by using agar diluiion method.The susceptibility of these IRPa to the antibiotics was analysised. Three-dimensional test was used to identify the different kinds of beta lactamases from 220 strains of Pa.2.Carbarpenems hydrolytic enzyme genes and oprD2 gene were detectedamong the selected IRPa strains, PCR method was performed to detect carbapenemase genes which included GES、KPC、SPM、VIM、IMP、GIM gene and the oprD2 gene;Multiplex PCR were used to detect OXA genes and plasmid-mediated AmpC beta lactamase genes; The expression of the chromosomal AmpC beta lactamases and oprD2 genes in IRPa strains were analyzed by Real-time PCR.3.Identification and characterization of integronsIntegrase gene was detected by PCR, and the classification of integrons was performed by using restriction fragment length polymorphism.PCR was performed to detect the qacE△1-sull gene,and the gene cassetes which are located at variable region of integrons in the strains were detected to be positive.

方法1、药敏实验和三维实验收集2003～2007年临床分离的220株Pa，对这些菌株采用K-B法测定10种临床常用抗生素的药敏情况，同时采用琼脂稀释法检测亚胺培南的最低抑菌浓度(Minimal inhibitory concentration，MIC)，筛选出对亚胺培南耐药的铜绿假单胞菌，并分析其对其它抗生素的药物敏感率；采用三维实验的方法分析220株Pa产β内酰胺酶的类型。2、碳青霉烯类水解酶和oprD2蛋白的检测针对鉴定的IRPa菌株，采用普通PCR方法检测具有碳青霉烯水解作用的β内酰胺酶耐药基因(GES、KPC、SPM、VIM、IMP、GIM基因)和oprD2基因，采用多重PCR的方法检测OXA型基因和质粒携带的AmpC酶基因，用荧光定量RT-PCR方法检测oprD2蛋白基因表达情况；同时对产AmpC酶的Pa(25株，含IMP耐药和敏感株)用RT-PCR方法检测AmpC酶基因的表达量情况。3。

In result, formic, acetic, methanesulfonic, oxalic, malonic, succinic and glutaric acids were detected notably. Formic, acetic and oxalic acids were the most abundant organic acids, and the sum of their concentrations averagely accounted for 89% of the total detected organic acids and 13% of the total anions, respectively.

结果表明，甲酸、乙酸、甲磺酸、乙二酸、丙二酸、丁二酸、戊二酸均有不同程度检出，一元有机酸中甲酸和乙酸在每场降水中均有检出；二元有机酸中，乙二酸和丁二酸检出率很高，其他有机酸检出率较低。

The results manifest that the concentration of glycocholic acid has satisfactory linear correlation whith peak area, regression equation: Y= 39579X + 57823,R2=0.9984;The lowest detected concentration is 0.02μg/mL with S/N=3 ; Average recovery rate is 98.49%; RSD为0.52%～2.00%in day, RSD为0.74%～4.98%between days;The average recovery rate is 99.35%, RSD is 2.24% in the first team, and it is 100.87%, RSD is 4.73% in the second team at the stability test. When the concentration of glycocholic acid was detected in the liver, kidney and brain tissue, corresponding tissue concentration was calculated by preparing the standard curve with the glycocholic acid standard preparation in vitro, but the result can only reflect the relative chang law of the concentration of glycocholic acid in tissue but not in real tissue. The result of tissue stability experiment is consistent with that of the serum experiment.

结果表明，在0.15625～10.0μg/ml范围内血清中甘氨胆酸浓度与峰面积呈良好的线性关系，回归方程Y= 39579X + 57823，R2=0.9984；以信噪比S/N=3为标准，最低检测浓度为0.02μg/ml；平均回收率为98.49%；日内RSD为0.52%～2.00%，日间RSD为0.74%～4.98%；稳定性试验第一组的平均含量为99.35%，RSD为2.24%，第二组的平均含量为100.87%，RSD为4.73%；测定了肝、肾、脑组织中甘氨胆酸含量，采用体外甘氨胆酸标准品制备标准曲线的方法计算了相应的组织浓度，其结果仅反映组织中甘氨胆酸浓度的相对变化规律，不能体现真正的组织浓度值，但对组织样品的稳定性进行了较细致的考察，其结果和血样结果基本一致。

A new algorithm of low bit rate image compression was proposed. Firstly, the edge informations of the input image were detected with SUSAN operator, then the detected edge informations were en hanced with edge enhancement method. Finally, the enhanced edge informations were added to the decoded input image to form the final compressed image.

提出一种新的低比特率图像压缩算法，该算法首先利用SUSAN算子检测出输入图像的边缘信息，然后利用边缘增强方法对边缘信息进行增强，最后将增强后的边缘信息和解码后的输入图像相加形成最终的压缩图像。

93 Target gene were detected by Tem-PCR from 75 specimen, they were: Hemophilus influenzae 40, Streptococcus penumoniae 36, Acinetobacter baumannii 10, Pseudomonas aeruginosa 4, Staphylococcus aureus 3, the other 9 kinds of bacterium including Escherichia coli、Klebsiella pneumoniae and Enterobactor cloacae were not detected by Tem-PCR, the positive rate of Tem-PCR was 39.9%(75/188).(3) For the 14 kinds of bacterium designed by Tem-PCR, compared with the culture, the sensitivity、specificity and coincidence of Tem-PCR is 51.0%, 68.0%, 58.3% respectively.

2经Tem-PCR技术扩增后，188例标本在Luminex100多功能悬浮点阵仪中有75例呈阳性，共检测出93株病原菌的靶基因，分别是流感嗜血杆菌40株，肺炎链球菌36株，鲍曼氏不动杆菌10株，铜绿假单胞菌4株，金黄色葡萄球菌3株，另外9种Tem-PCR已设计的细菌包括肺炎克雷伯菌、大肠杆菌、阴沟肠杆菌等均未检出，Tem-PCR的阳性率是39.9%(75/188)。

Methods From Feb 2006 to Jun 2006,188 hospitalized children in Shenzhen children s hospital, were collected deep tracheal aspirate at the time of hospitalization. The respiratory tract secretions were immediately sent for bacterial culture with 3 kinds of medium:ordinary medium, Hemophilus influenzae selective medium, Streptococcus penumoniae selective medium. Then we extracted the total nucleic acids from secretions, and detected Mycoplasma pneumoniae by single fluorescent quantitation PCR. Simultaneously, 14 respiratory tract pathogenic bacterium and Mycoplasma pneumoniae were detected by Target Enriched multiplex PCR. Amplification products were identified by the Luminex100 suspension array.

确诊为社区获得性肺炎的患儿188例，在入院当天采集深部呼吸道吸引物，用普通培养基和肺炎链球菌、流感嗜血杆菌选择性培养基进行细菌培养，然后提取深部呼吸道吸引物中病原体的DNA，采用荧光定量单PCR的方法检测肺炎支原体，并对同一标本采用靶序列富集多重PCR技术同时扩增肺炎链球菌、流感嗜血杆菌、金黄色葡萄球菌、肺炎克雷伯菌、大肠杆菌、嗜肺军团菌、铜绿假单胞菌、鲍曼氏不动杆菌、脑膜炎奈瑟氏菌、阴沟肠杆菌、奇异变形杆菌、化脓链球菌、粪肠球菌及屎肠球菌14种呼吸道病原菌和肺炎支原体的靶基因，扩增产物用Luminex100多功能悬浮点阵仪检测。

Kinds of fatty acids were detected by method 1 and 24 fatty acids were detected by method 2. The results showed that the main components of the fatty acids in pomegranate seeds are: octadecenoic acid, octadecdienoic acid, docosanoic acid, hexadecanoic acid, octadecanoic acid, eicosenoic acid and eicosanoic acid.

方法一共检测出14种脂肪酸，主成分为油酸、亚油酸、山嵛酸、棕桐酸、硬脂酸和二十碳烯酸；方法二共检测出24种脂肪酸，主成分为棕橺酸、硬脂酸、花生酸、山嵛酸、油酸、亚油酸、二十碳烯酸等。

RT-PCR analysis showed that GhMADS1 gene expressed in petals, stamens, ovules and fibers, but not in roots, stems, leaves, bracts and sepals. The strongest expression of GhMADS1 gene was detected in petals. But in floral buds of a cotton homeotic mutant (CHV1), whose floral organs are all converted to bract leaf-like organs, the transcript of GhMADS1 gene was not detected.

RT-PCR分析显示，该基因在陆地棉的花瓣、雄蕊、胚珠和纤维中表达，特别是在花瓣中表达量最高，而在根、茎、叶等营养器官和棉花同源异型突变体CHV1（所有花器官均变为苞叶状叶性器官）的变异花蕾中不表达。

RT-PCR analysis showed the GhMASD1 gene expressed in petals, stamens, ovules and fibers, but not in roots,stems, leaves, bracts and sepals. The strongest expression of GhMADS1 gene was detected in petals. But in floral buds of a cotton homeotic mutant (CHV1), whose floral orgens are all converted to bract leaf-like organs, the transcript of GhMADS1 gene was not detected.

RT-PCR分析显示，该基因在陆地棉的花瓣、雄蕊、胚珠和纤维中表达，特别是在花瓣中表达量最高，而在根、茎、叶等营养器官和棉花同源异型突变体CHV1（所有花器官均变为苞叶状叶性器官）的变异花蕾中不表达。

Methods The 26th exon, the 30th exon and the 21st intron of gene GP Ⅱb in 110 individuals were amplified by polymorphism and sequenced to investigate whether there was linkage among the polymorphisms of the gene. Human platelet antigen-3 (HPA-3) gene frequency was detected by Fok 1 enzyme in 147 patients with hematologic diseases, and was compared with that in 110 normal individuals. Forty-four patients who received apheresis platelet transfusion repeatedly were randomly divided into the HPA-3 homotype group and the control group. The antibodies of the platelet were detected after 3 times of platelet transfusion.

聚合酶链反应－单链构型多态性分析检测110例正常人GP Ⅱb基因第26和30外显子(Exon 26， Exon 30)及21内含子(Intron21)基因多态性，进行基因序分析，研究这些基因多态性是否存在连锁关系；应用Fok1酶切法对147例血液病人人类血小板抗原－3(human platelet antigen-3， HPA-3)基因进行分型，并与110例正常人进行比较；将接受单采血小板输注的44例血液病人随机分为HPA-3同型输注组和对照组，血小板输注3次以后检则血小板抗体。

But GST= 0.156, Nm=1. 588. As a result, the foundation of Youyongchi Avicennia marina population was the result of the migration of hypocotyles and human factors.

这项工作可以为海岸防护林中新引进种类的判定以及为研究种群建立者效应方法的确定提供科学依据。

The two-dimensional CDRC-ADI-FDTD update equations for collision unmagnetized plasma are induced. The unconditional stability of the CDRC-ADI-FDTD formulation for collision unmagnetized plasma is obtained by the examples.

推导了碰撞非磁化等离子体中的二维CDRC-ADI-FDTD迭代公式，并用算例验证了碰撞非磁化等离子体CDRC-ADI-FDTD算法也是无条件稳定的。