查询词典 current amplification factor

In this model,the molecular basis that PCR reaction is doomed to change from exponential amplification to linear amplification had been clarified.

本模型阐明了PCR反应在多次循环后必然由指数扩增转变为线性扩增的分子基础，为定量PCR 提供了准确的计算方法。

After that, we detect cell DNA heteroploid and cell cycle transformation of human osteosarcoma tissue by using flow cytometry method; next, we amplification the exon2 and exon3 of 〓 gene in 36 cases human osteosarcoma tissue, and amplification products were performed the sepharose and polyacrylamide gel electrophoresis immediately.

然后采用原位杂交方法，对45例骨肉瘤标本组织和10例骨纤维结构不良组织中〓基因mRNA进行检测，以了解骨肉瘤标本组织和骨纤维结构不良组织中〓基因在蛋白质水平和mRNA水平的表达情况。

A method for detecting the presence of a target nucleic acid sequence in a sample, said method comprising subjecting said sample to an amplification reaction using a set of nucleotides, at least one of which is fluorescently labelled, contacting amplification product with a probe under conditions in which the probe will hybridise to said target sequence, said probe comprising a reactive molecule which is able to absorb fluorescence from or donate fluorescent energy to said fluorescent labelled nucleotide and monitoring fluorescence of said sample.

一种用于检测在某样品中是否存在目标核酸序列的方法，该方法包括：使用一套核苷酸对所说的样品进行扩增反应，其中至少一个核苷酸是被荧光标记的，在探针与目标序列杂交的条件下使扩增产物与探针混合，所说的探针包括一种活性分子，该活性分子能够从荧光标记核苷酸吸收荧光或向其贡献荧光能量，及检测样品的荧光。

22 Of 22 pairs EST-SSR primers from Chinese cabbage/rape respectively had amplification products in soybean,wheat and maize,the rate of amplification was 81.8%,95.4%,100%,respectively;10 of 22 pairs EST-SSR primers showed polymorphism in monocotyledons wheat and maize,13 in dicotyledons oilseed rape and soybean,7 in both monocotyledonous and dicotyledonous crops.

（1）30对小麦EST-SSR引物中，有29，28和26对引物分别在玉米、油菜和大豆中有扩增产物，可扩增率分别为96.7%，93.3%和86.7%；其中分别有12对和9对引物在单子叶作物小麦和玉米及双子叶作物大豆和油菜中的扩增产物显示多态性，有4对引物在4种作物中的扩增产物均显示多态性。

(1)29,28,26 of 30 pairs of EST-SSR primers from wheat respectively had amplification products in maize,oilseed rape and soybean,the rate of amplification was 96.7%,93.3%,86.7%,respectively;12 of 30 pairs EST-SSR primers from wheat showed polymorphism in monocotyledons wheat and maize,9 in dicotyledons oilseed rape and soybean,and 4 in both monocotyledonous and dicotyledonous crops.

基金项目：国家生命科学与技术人才培养基地科技创新基金项目(2007088)；国家油菜产业化项目科学家岗位"化杀岗位"（nycytx-00505）；国家自然科学基金项目(30871578)中文摘要：探讨单、双子叶作物间EST-SSR引物和标记的通用性。选取30对小麦、8对油菜和14对白菜的EST-SSR引物，分别以10个小麦、10个油菜、5个大豆品种和5个玉米自交系的基因组DNA为模板，进行PCR扩增及扩增产物的多态性分析。

The method is based on real time reverse-transcription-PCR amplification of mRNA to detect viable Listeria monocytogenes by specific hlyA gene. This method can overcome the false-positive caused by amplification of nonviable Listeria monocytogenes in the detection process.

为克服传统PCR检测的假阳性问题，有效的检测单增李斯特活菌，本研究提取单增李斯特菌的总RNA并进行反转录，以单增李斯特菌的必要毒力基因hlyA为靶基因，设计特异性引物和TaqMan探针进行实时PCR检测，研究了检测的特异性和灵敏度，考查了对人工污染牛乳进行检测的应用性。

By analyzing the optical characteristic of the off-axis and multi-pass amplified high power solid-state laser facility,using the theory of binary tree tracing and matrix optics,and dividing the system into four parts that include cavity amplification part,boost amplification part,beam reverser and beam output part,the ghost image are analyzed and traced.Main of ghost image that are devastating are given in all parts.

为了有效地避免鬼像破坏，针对采用离轴多程放大的高功率固体激光实验装置的光路特点，利用"光路二叉树"原理和"矩阵光学"方法，将全光路"单元化"为主放大、助推放大、反转器和输出4个部分进行光线追迹和鬼像分析，给出了各个部分的主要鬼像分布。

Seeded amplification of colored conical emission and two-dimensional multicolored transverse arrays as well as simultaneously up-converted parametric amplification have been demonstrated using spectral synthesis to obtain sub-fs pulses.

基于分子调制过程，文章作者提出并发展了一系列亚飞秒脉冲产生和操控的技术；围绕频谱合成方案，利用级联二阶非线性效应，实现了多色圆锥辐射注入放大和二维多色阵列及其参量上转换放大；同时，还开展了基于飞秒振荡器及外腔增强技术的高重复频率高次谐波产生与输出耦合的相关研究。

The reaction system of AFLP (Amplified Fragment Length Polymorphism) in genus Eriobotrya was studied and the AFLP analysis system was established by detected the results of several reactions such as DNA extraction, enzymes restriction, adapter-ligation, pre-amplification and selective amplification; the main factors which affected AFLP analysis were analyzed.

通过检测AFLP分析中DNA提取、酶切及连接、预扩增和选择性扩增的结果，对影响AFLP分析的主要因子进行分析，建立了AFLP分析体系。

Using the amplification transfer function of type Ⅰ BBO crystal, the theoretical study of spatial properties of the optical parametric fluorescence generated by homochromatic signal and polychromatic signal in optical parametric amplification is investigated, The numerical simulation results demonstrate that when the homochromatic signal is injected into optical parametric amplifier, the fluorescence signal is first emitted in a cone and then in a ring while centering on the pump wave direction.

采用第Ⅰ类相位匹配BBO晶体的放大传递函数方法，分别对光参量放大过程中单波长信号光注入和多波长信号光注入出现的参量荧光的空间特性进行了详细的理论研究。

The studies on the developing of new media are very important to the increasing of the area density of HDD and to the implementation of hybrid recoding technology.

光磁混合记录方法，是一种可以突破超顺磁极限的限制，并进一步提高硬盘记录密度和读写速率的一种新型超高密度信息存储方式。

In io, mTOR inhibition promotes T cell anergy under conditions that would normally induce priming.

体内研究中，在正常可激发免疫反应的条件下，抑制mTOR可促使T细胞无能。